Strain identifier

BacDive ID: 1896

Type strain: Yes

Species: Brucella pituitosa

Strain history: <- H.-J. Busse, Univ. Veterinary Medicine Vienna, Austria; CCUG 50899 <- H. C. Scholz <- E. Falsen

NCBI tax ID(s): 571256 (species)

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General

@ref: 16203

BacDive-ID: 1896

DSM-Number: 22207

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Brucella pituitosa DSM 22207 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from industrial environment.

NCBI tax id

  • NCBI tax id: 571256
  • Matching level: species

strain history

  • @ref: 16203
  • history: <- H.-J. Busse, Univ. Veterinary Medicine Vienna, Austria; CCUG 50899 <- H. C. Scholz <- E. Falsen

doi: 10.13145/bacdive1896.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Brucellaceae
  • genus: Brucella
  • species: Brucella pituitosa
  • full scientific name: Brucella pituitosa (Huber et al. 2010) Hördt et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Ochrobactrum pituitosum

@ref: 16203

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Brucellaceae

genus: Brucella

species: Brucella pituitosa

full scientific name: Brucella pituitosa (Huber et al. 2010) Hördt et al. 2020

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotilityconfidence
23343negative1.5-2.5 µmrod-shapedyes
69480negative99.974

colony morphology

  • @ref: 23343
  • colony size: 1 mm
  • colony color: beige, translucent
  • colony shape: circular
  • incubation period: 1 day

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16203R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
23343Columbia agaryessheep blood
23343MacConkeyyes
23343Nutrient agar (NA)yes
23343PYE agaryes
23343Reasoner's 2A agar (R2A)yes
23343Trypticase Soy Agar (TSA)yes

culture temp

@refgrowthtypetemperaturerange
16203positivegrowth30mesophilic
23343positivegrowth25-30mesophilic
23343inconsistentgrowth20psychrophilic
23343inconsistentgrowth35mesophilic
23343nogrowth4psychrophilic
23343nogrowth>35mesophilic
59044positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23343aerobe
59044aerobe

spore formation

@refspore formationconfidence
23343no
69481no100
69480no99.985

halophily

@refsaltgrowthtested relationconcentration
23343NaClpositivegrowth6 %
23343NaClnogrowth7 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23343167632-oxobutanoate-assimilation
23343181014-hydroxyphenylacetic acid-assimilation
233438391D-gluconate-assimilation
2334332323glucuronamide-assimilation
2334317754glycerol-assimilation
2334321217L-alaninamide-assimilation
2334316857L-threonine-assimilation
23343506227N-acetylglucosamine-assimilation
2334316634raffinose-assimilation
2334341865sebacic acid-assimilation
23343143136succinamate-assimilation
2334327082trehalose-assimilation
2334315963ribitol-builds acid from
2334317057cellobiose-builds acid from
2334317268myo-inositol-builds acid from
2334317716lactose-builds acid from
2334328053melibiose-builds acid from
2334326546rhamnose-builds acid from
233433122bis-4-nitrophenyl phosphate-hydrolysis
2334316383cis-aconitate+assimilation
2334315971L-histidine+assimilation
2334315603L-leucine+assimilation
2334368428maltitol+assimilation
2334317306maltose+assimilation
2334315708trans-aconitate+assimilation
2334327082trehalose+assimilation
2334365327D-xylose+builds acid from
2334330849L-arabinose+builds acid from
23343gamma-L-glutamate-4-nitroanilide+hydrolysis
2334391050p-nitrophenyl phenyl phosphonate+hydrolysis
2334315963ribitol+assimilation
2334317057cellobiose+assimilation
2334316947citrate+assimilation
2334318333D-arabitol+assimilation
2334317784D-glucosaminic acid+assimilation
2334316899D-mannitol+assimilation
2334317924D-sorbitol+assimilation
23343167244-hydroxybutyrate+assimilation
2334328066gentiobiose+assimilation
2334318287L-fucose+assimilation
2334362345L-rhamnose+assimilation
2334317268myo-inositol+assimilation
2334328037N-acetylgalactosamine+assimilation
2334317992sucrose+assimilation
2334353426tween 80+assimilation
2334316704uridine+assimilation
2334317113erythritol+/-builds acid from
23343911224-nitrophenyl alpha-D-glucopyranoside+/-hydrolysis
23343902594-nitrophenyl beta-D-glucopyranoside+/-hydrolysis
6836925115malate+assimilation
6836917128adipate-assimilation
6836924265gluconate-assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
23343catalase+1.11.1.6
23343cytochrome oxidase+1.9.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
16203+---+---+++-++-+/--++-+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
16203industrial environmentSwedenSWEEurope
59044Industry,environmentSwedenSWEEurope2005-04-01

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Industrial

Safety information

risk assessment

  • @ref: 16203
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16203
  • description: Ochrobactrum pituitosum partial 16S rRNA gene, strain CCUG 50899
  • accession: AM490609
  • length: 1429
  • database: ena
  • NCBI tax ID: 571256

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brucella pituitosa CCUG 50899GCA_003049685contigncbi571256
66792Brucella pituitosa CCUG 50899GCA_008801705contigncbi571256
66792Ochrobactrum pituitosum strain CCUG 50899571256.25wgspatric571256
66792Ochrobactrum pituitosum strain CCUG 50899571256.19wgspatric571256

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.701yes
anaerobicno98.12yes
halophileno93.896no
spore-formingno96.601no
glucose-utilyes93.5no
thermophileno99.055no
flagellatedno82.262no
aerobicyes91.777yes
motileyes83.368yes
glucose-fermentno90.045yes

External links

@ref: 16203

culture collection no.: DSM 22207, CCUG 50899

straininfo link

  • @ref: 71540
  • straininfo: 214848

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19651740Ochrobactrum pituitosum sp. nov., isolated from an industrial environment.Huber B, Scholz HC, Kampfer P, Falsen E, Langer S, Busse HJInt J Syst Evol Microbiol10.1099/ijs.0.011668-02009Bacterial Proteins/genetics, *Environmental Microbiology, Industry, Molecular Sequence Data, Ochrobactrum/*classification/genetics/physiology, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sequence Homology, Nucleic Acid, SwedenGenetics
Phylogeny21169456Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant.Woo SG, Ten LN, Park J, Lee MInt J Syst Evol Microbiol10.1099/ijs.0.025510-02010Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Nitrates/*metabolism, Ochrobactrum/*classification/genetics/*isolation & purification/metabolism, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sewage/*microbiology, Waste Disposal, FluidMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16203Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22207)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22207
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23343Birgit Huber, Holger C. Scholz, Peter Kämpfer, Enevold Falsen, Stefan Langer, Hans-Jürgen Busse10.1099/ijs.0.011668-0Ochrobactrum pituitosum sp. nov., isolated from an industrial environmentIJSEM 60: 321-326 201019651740
59044Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 50899)https://www.ccug.se/strain?id=50899
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71540Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID214848.1StrainInfo: A central database for resolving microbial strain identifiers