Strain identifier
BacDive ID: 1893
Type strain:
Species: Brucella haematophila
Strain Designation: Falu B 57617/97
Strain history: CIP <- 2007, CCUG <- 1997 O.Nyberg, Falun, Sweden: strain Falu B 57617/97
NCBI tax ID(s): 419474 (species)
General
@ref: 16179
BacDive-ID: 1893
DSM-Number: 22355
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming, human pathogen
description: Brucella haematophila Falu B 57617/97 is an obligate aerobe, mesophilic, Gram-negative human pathogen that forms circular colonies and was isolated from blood of a 79-year-old man.
NCBI tax id
- NCBI tax id: 419474
- Matching level: species
strain history
@ref | history |
---|---|
16179 | <- CIP <- CCUG |
37561 | 2007, CCUG |
119762 | CIP <- 2007, CCUG <- 1997 O.Nyberg, Falun, Sweden: strain Falu B 57617/97 |
doi: 10.13145/bacdive1893.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Brucellaceae
- genus: Brucella
- species: Brucella haematophila
- full scientific name: Brucella haematophila (Kämpfer et al. 2007) Hördt et al. 2020
synonyms
- @ref: 20215
- synonym: Ochrobactrum haematophilum
@ref: 16179
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Brucellaceae
genus: Brucella
species: Brucella haematophila
full scientific name: Brucella haematophila (Kämpfer et al. 2007) Hördt et al. 2020
strain designation: Falu B 57617/97
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell shape | motility | confidence |
---|---|---|---|---|---|
23341 | negative | 2 µm | rod-shaped | no | |
69480 | negative | 99.989 | |||
119762 | negative | rod-shaped | no |
colony morphology
- @ref: 23341
- colony size: 2 mm
- colony color: beige, translucent
- colony shape: circular
- incubation period: 1 day
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
16179 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | https://mediadive.dsmz.de/medium/220 | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water |
23341 | MacConkey | yes | ||
23341 | Nutrient agar (NA) | yes | ||
23341 | PYE agar | yes | ||
23341 | Reasoner's 2A agar (R2A) | yes | ||
23341 | Trypticase Soy Agar (TSA) | yes | ||
37561 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
119762 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
16179 | positive | growth | 30 | mesophilic |
23341 | positive | growth | 25-30 | mesophilic |
23341 | no | growth | 4 | psychrophilic |
23341 | no | growth | 42 | thermophilic |
37561 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 119762
- oxygen tolerance: obligate aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
23341 | no | |
69481 | no | 100 |
69480 | no | 99.991 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
23341 | NaCl | positive | growth | 5 % |
23341 | NaCl | no | growth | 7 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23341 | 17057 | cellobiose | - | assimilation |
23341 | 28037 | N-acetylgalactosamine | - | assimilation |
23341 | 15708 | trans-aconitate | - | assimilation |
23341 | gamma-L-glutamate-4-nitroanilide | - | hydrolysis | |
23341 | 91050 | p-nitrophenyl phenyl phosphonate | - | hydrolysis |
23341 | 16865 | gamma-aminobutyric acid | + | assimilation |
23341 | 17879 | 4-hydroxybenzoate | + | assimilation |
23341 | 15963 | ribitol | + | assimilation |
23341 | 16958 | beta-alanine | + | assimilation |
23341 | 16383 | cis-aconitate | + | assimilation |
23341 | 16947 | citrate | + | assimilation |
23341 | 15824 | D-fructose | + | assimilation |
23341 | 17306 | maltose | + | assimilation |
23341 | 17924 | D-sorbitol | + | assimilation |
23341 | 37054 | 3-hydroxybutyrate | + | assimilation |
23341 | 29991 | L-aspartate | + | assimilation |
23341 | 15971 | L-histidine | + | assimilation |
23341 | 15603 | L-leucine | + | assimilation |
23341 | 62345 | L-rhamnose | + | assimilation |
23341 | 68428 | maltitol | + | assimilation |
23341 | 17268 | myo-inositol | + | assimilation |
23341 | 506227 | N-acetylglucosamine | + | assimilation |
23341 | 9300 | suberic acid | + | assimilation |
23341 | 17992 | sucrose | + | assimilation |
23341 | 27082 | trehalose | + | assimilation |
23341 | L-proline-4-nitroanilide | + | hydrolysis | |
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | 24265 | gluconate | - | builds acid from |
68371 | 18403 | L-arabitol | - | builds acid from |
68371 | 18333 | D-arabitol | - | builds acid from |
68371 | 62318 | D-lyxose | - | builds acid from |
68371 | 32528 | turanose | - | builds acid from |
68371 | 28066 | gentiobiose | - | builds acid from |
68371 | 17151 | xylitol | - | builds acid from |
68371 | 28087 | glycogen | - | builds acid from |
68371 | 28017 | starch | - | builds acid from |
68371 | 16634 | raffinose | - | builds acid from |
68371 | 6731 | melezitose | - | builds acid from |
68371 | 15443 | inulin | - | builds acid from |
68371 | 27082 | trehalose | - | builds acid from |
68371 | 17992 | sucrose | - | builds acid from |
68371 | 28053 | melibiose | - | builds acid from |
68371 | 17716 | lactose | - | builds acid from |
68371 | 17306 | maltose | - | builds acid from |
68371 | 17057 | cellobiose | - | builds acid from |
68371 | 17814 | salicin | - | builds acid from |
68371 | 4853 | esculin | - | builds acid from |
68371 | 18305 | arbutin | - | builds acid from |
68371 | 27613 | amygdalin | - | builds acid from |
68371 | 59640 | N-acetylglucosamine | - | builds acid from |
68371 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
68371 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
68371 | 17924 | D-sorbitol | - | builds acid from |
68371 | 16899 | D-mannitol | - | builds acid from |
68371 | 17268 | myo-inositol | - | builds acid from |
68371 | 16813 | galactitol | - | builds acid from |
68371 | 62345 | L-rhamnose | - | builds acid from |
68371 | 17266 | L-sorbose | - | builds acid from |
68371 | 16024 | D-mannose | - | builds acid from |
68371 | 15824 | D-fructose | - | builds acid from |
68371 | 17634 | D-glucose | - | builds acid from |
68371 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
68371 | 15963 | ribitol | - | builds acid from |
68371 | 65328 | L-xylose | - | builds acid from |
68371 | 65327 | D-xylose | - | builds acid from |
68371 | 16988 | D-ribose | - | builds acid from |
68371 | 30849 | L-arabinose | - | builds acid from |
68371 | 17108 | D-arabinose | - | builds acid from |
68371 | 17113 | erythritol | - | builds acid from |
68371 | 17754 | glycerol | - | builds acid from |
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 27689 | decanoate | - | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | + | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | + | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
119762 | 17632 | nitrate | - | reduction |
119762 | 16301 | nitrite | - | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
119762 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
16179 | cytochrome-c oxidase | + | 1.9.3.1 |
23341 | cytochrome oxidase | + | 1.9.3.1 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | + | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68369 | cytochrome oxidase | + | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
119762 | oxidase | + | |
119762 | catalase | + | 1.11.1.6 |
119762 | urease | + | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119762 | - | + | + | - | - | + | - | - | + | - | + | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
16179 | - | - | - | - | - | - | - | - | + | + | + | +/- | + | + | + | - | - | + | + | - | + |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119762 | - | - | - | - | - | - | - | - | - | +/- | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | +/- | +/- | +/- | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date |
---|---|---|---|---|---|---|
16179 | blood of a 79-year-old man | Falun | Sweden | SWE | Europe | |
54314 | Human blood,79-yr-old man | Falun | Sweden | SWE | Europe | 1997-09-20 |
119762 | Human, Blood | Falun | Sweden | SWE | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Patient | |
#Host | #Human | #Male |
#Host Body Product | #Fluids | #Blood |
taxonmaps
- @ref: 69479
- File name: preview.99_2618.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_45;97_1426;98_2027;99_2618&stattab=map
- Last taxonomy: Ochrobactrum
- 16S sequence: AM422370
- Sequence Identity:
- Total samples: 6752
- soil counts: 1466
- aquatic counts: 912
- animal counts: 3231
- plant counts: 1143
Safety information
risk assessment
@ref | pathogenicity human | biosafety level | biosafety level comment |
---|---|---|---|
16179 | yes, in single cases | 1 | Risk group (German classification) |
119762 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Ochrobactrum anthropi partial 16S rRNA gene, strain CCUG 38531 | AM114405 | 1388 | ena | 529 |
16179 | Ochrobactrum haematophilum partial 16S rRNA gene, type strain CCUG 38531T | AM422370 | 1388 | ena | 419474 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Brucella haematophila CCUG 38531 | GCA_005938105 | contig | ncbi | 419474 |
66792 | Brucella haematophila CIP 109452 | GCA_012103105 | contig | ncbi | 419474 |
66792 | Ochrobactrum haematophilum strain CCUG 38531 | 419474.10 | wgs | patric | 419474 |
66792 | Ochrobactrum haematophilum strain CIP 109452 | 419474.11 | wgs | patric | 419474 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
gram-positive | no | 98.275 | yes |
anaerobic | no | 96.72 | no |
halophile | no | 92.407 | no |
spore-forming | no | 95.745 | no |
glucose-util | yes | 94.854 | yes |
aerobic | yes | 92.013 | no |
flagellated | no | 78.62 | yes |
motile | yes | 81.397 | no |
thermophile | no | 99.084 | no |
glucose-ferment | no | 90.282 | yes |
External links
@ref: 16179
culture collection no.: DSM 22355, CCUG 38531, CIP 109452
straininfo link
- @ref: 71537
- straininfo: 56007
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 17978211 | Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens. | Kampfer P, Scholz HC, Huber B, Falsen E, Busse HJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.65066-0 | 2007 | Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Genotype, Gram-Negative Bacterial Infections/microbiology, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Ochrobactrum/chemistry/*classification/genetics/physiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA | Genetics |
Phylogeny | 32100690 | Ochrobactrum teleogrylli sp. nov., a pesticide-degrading bacterium isolated from the insect Teleogryllus occipitalis living in deserted cropland. | Hu M, Li X, Li Z, Liu B, Yang Z, Tian Y | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003964 | 2020 | Agriculture, Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gryllidae/*microbiology, Nucleic Acid Hybridization, Ochrobactrum/*classification/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
16179 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22355) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22355 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23341 | Peter Kämpfer, Holger C. Scholz, Birgit Huber, Enevold Falsen, Hans-Jürgen Busse | 10.1099/ijs.0.65066-0 | Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens | IJSEM 57: 2513-2518 2007 | 17978211 | |
37561 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7197 | ||||
54314 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 38531) | https://www.ccug.se/strain?id=38531 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68369 | Automatically annotated from API 20NE | |||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
71537 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID56007.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119762 | Curators of the CIP | Collection of Institut Pasteur (CIP 109452) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109452 |