Strain identifier

BacDive ID: 1891

Type strain: Yes

Species: Brucella pseudintermedia

Strain history: 2006, C. Teyssier, Montpellier Univ., Montpellier, France: strain ADV31

NCBI tax ID(s): 370111 (species)

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General

@ref: 7018

BacDive-ID: 1891

DSM-Number: 17490

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, human pathogen

description: Brucella pseudintermedia CCUG 55422 is an aerobe, mesophilic, Gram-negative human pathogen that was isolated from human axillary swab.

NCBI tax id

  • NCBI tax id: 370111
  • Matching level: species

strain history

@refhistory
7018<- C. Teyssier; ADV31
383822006, C. Teyssier, Montpellier Univ., Montpellier, France: strain ADV31

doi: 10.13145/bacdive1891.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Brucellaceae
  • genus: Brucella
  • species: Brucella pseudintermedia
  • full scientific name: Brucella pseudintermedia (Teyssier et al. 2007) Hördt et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Ochrobactrum pseudintermedium

@ref: 7018

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Brucellaceae

genus: Brucella

species: Brucella pseudintermedia

full scientific name: Brucella pseudintermedia (Teyssier et al. 2007) Hördt et al. 2020

type strain: yes

Morphology

cell morphology

  • @ref: 23340
  • gram stain: negative
  • cell length: 1.5-1.7 µm
  • cell width: 0.65-0.75 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: monotrichous, polar

colony morphology

  • @ref: 23340
  • colony color: opaque

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7018TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
23340MacConkeyyes
23340Reasoner's 2A agar (R2A)yes
38382MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@refgrowthtypetemperaturerange
7018positivegrowth37mesophilic
23340positivegrowth25-45
38382positivegrowth37mesophilic
60582positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23340aerobe
60582aerobe

spore formation

  • @ref: 23340
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2334017128adipate-assimilation
2334015971L-histidine-assimilation
2334024996lactate-assimilation
2334018401phenylacetate-assimilation
23340174265-dehydro-D-gluconate-builds acid from
2334023399coumarate-builds acid from
2334017057cellobiose-builds acid from
2334017634D-glucose-builds acid from
2334016899D-mannitol-builds acid from
2334016024D-mannose-builds acid from
2334016551D-trehalose-builds acid from
2334018403L-arabitol-builds acid from
2334015792malonate-builds acid from
233405291gelatin-hydrolysis
2334022599arabinose+assimilation
2334027689decanoate+assimilation
2334017306maltose+assimilation
2334016899D-mannitol+assimilation
2334017234glucose+assimilation
2334025115malate+assimilation
2334037684mannose+assimilation
23340506227N-acetylglucosamine+assimilation
2334032032potassium gluconate+assimilation
2334015963ribitol+builds acid from
2334016443D-tagatose+builds acid from
2334024996lactate+builds base from
2334030031succinate+builds base from
2334017632nitrate+reduction
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
2334017698chloramphenicolyesyes
2334037943colistinyesyes
2334028915fosfomycinyesyes
2334027902tetracyclineyesyes
233402637amikacinyesyes
23340100241ciprofloxacinyesyes
233403770co-trimoxazoleyesyes
2334017833gentamicinyesyes
23340471744imipenemyesyes
2334037951isepamicinyesyes
23340100147nalidixic acidyesyes
233407528netilmycinyesyes
233407731ofloxacinyesyes
2334050199pefloxacinyesyes
2334028077rifampicinyesyes
2334028864tobramycinyesyes

metabolite production

@refChebi-IDmetaboliteproduction
2334035581indoleno
2334016136hydrogen sulfideno
2334015688acetoinno
6836935581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
2334015688acetoin-
6836935581indole-

enzymes

@refvalueactivityec
23340alanine arylamidase-3.4.11.2
23340alpha-galactosidase-3.2.1.22
23340arginine dihydrolase-3.5.3.6
23340beta-galactosidase-3.2.1.23
23340beta-glucosidase-3.2.1.21
23340beta-glucuronidase-3.2.1.31
23340catalase+1.11.1.6
23340cytochrome oxidase+1.9.3.1
23340DNase-
23340leucyl glycin arylamidase+3.4.11.1
23340lipase-
23340lysine decarboxylase-4.1.1.18
23340N-acetyl-beta-glucosaminidase-3.2.1.52
23340ornithine decarboxylase-4.1.1.17
23340phenylalanine deaminase-4.3.1.5
23340phosphatase-
23340proline-arylamidase+3.4.11.5
23340pyrrolidonyl arylamidase+3.4.19.3
23340tryptophan deaminase-4.1.99.1
23340tyrosine arylamidase+
23340urease-3.5.1.5
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
7018+----+--++++++++-+--+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
7018human axillary swabMontpellierFranceFRAEurope
23340human clinical sample (axillary swab) from a 20 year old man
60582Human axillary swab,20-yr-old manMontpellierFranceFRAEurope2002

isolation source categories

Cat1Cat2Cat3
#Infection#Medical environment#Clinic
#Infection#Patient#Swab
#Host#Human#Male

Safety information

risk assessment

  • @ref: 7018
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
23340Ochrobactrum pseudintermedium ADV31 16S ribosomal RNA gene, partial sequenceDQ3659211396nuccore370111
7018Ochrobactrum pseudintermedium ADV43 16S ribosomal RNA gene, partial sequenceDQ3659231379ena370111

GC content

@refGC-contentmethod
701854.5
2334054.5high performance liquid chromatography (HPLC)

External links

@ref: 7018

culture collection no.: CCUG 55422, DSM 17490, CIP 109116, KCTC 22760, ADV 31

straininfo link

  • @ref: 71535
  • straininfo: 308621

literature

  • topic: Phylogeny
  • Pubmed-ID: 17473249
  • title: Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples.
  • authors: Teyssier C, Marchandin H, Jean-Pierre H, Masnou A, Dusart G, Jumas-Bilak E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64416-0
  • year: 2007
  • mesh: Bacterial Proteins/genetics, Bacterial Typing Techniques, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA-Directed RNA Polymerases/metabolism, Fatty Acids/analysis, Genes, rRNA/genetics, Gram-Negative Bacterial Infections/*microbiology, Humans, Membrane Lipids/analysis, Molecular Sequence Data, Ochrobactrum/chemistry/*classification/*isolation & purification/physiology, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7018Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17490)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17490
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23340Corinne Teyssier, Hélène Marchandin, Hélène Jean-Pierre, Agnès Masnou, Ghislaine Dusart, Estelle Jumas-Bilak10.1099/ijs.0.64416-0Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samplesIJSEM 57: 1007-1013 200717473249
38382Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6822
60582Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 55422)https://www.ccug.se/strain?id=55422
68369Automatically annotated from API 20NE
71535Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID308621.1StrainInfo: A central database for resolving microbial strain identifiers