Strain identifier
BacDive ID: 1889
Type strain: 
Species: Brucella lupini
Strain history: <- M. E. Trujillo; LUP21
NCBI tax ID(s): 255457 (species)
version 8.1 (current version)
General
@ref: 6650
BacDive-ID: 1889
DSM-Number: 16930
keywords: 16S sequence, Bacteria, aerobe, mesophilic, human pathogen
description: Brucella lupini CCUG 54647 is an aerobe, mesophilic human pathogen that was isolated from Lupinus honoratus root nodules.
NCBI tax id
- NCBI tax id: 255457
- Matching level: species
strain history
- @ref: 6650
- history: <- M. E. Trujillo; LUP21
doi: 10.13145/bacdive1889.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Brucellaceae
- genus: Brucella
- species: Brucella lupini
- full scientific name: Brucella lupini (Trujillo et al. 2006) Hördt et al. 2020
synonyms
- @ref: 20215
- synonym: Ochrobactrum lupini
@ref: 6650
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Brucellaceae
genus: Brucella
species: Brucella lupini
full scientific name: Brucella lupini (Trujillo et al. 2006) Hördt et al. 2020
type strain: no
Morphology
colony morphology
- @ref: 6650
- incubation period: 1-2 days
Culture and growth conditions
culture medium
- @ref: 6650
- name: NUTRIENT AGAR (DSMZ Medium 1)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1
- composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6650 | positive | growth | 28 | mesophilic |
| 60280 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 60280
- oxygen tolerance: aerobe
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | + | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | + | assimilation |
| 68369 | 30849 | L-arabinose | + | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | + | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 6650 | catalase | + | 1.11.1.6 |
| 6650 | cytochrome-c oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | + | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 6650 | + | - | - | - | + | - | - | - | + | + | + | + | + | + | + | + | - | + | + | - |
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | country | origin.country | continent | geographic location |
|---|---|---|---|---|---|---|
| 6650 | Lupinus honoratus root nodules | Lupinus honoratus | Argentina | ARG | Middle and South America | |
| 60280 | Nodules,Lupinus honoratus | Argentina | ARG | Middle and South America | Córdoba |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
| #Host Body-Site | #Plant | #Root nodule |
taxonmaps
- @ref: 69479
- File name: preview.99_2226.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_45;97_1426;98_1736;99_2226&stattab=map
- Last taxonomy: Ochrobactrum
- 16S sequence: AY457038
- Sequence Identity:
- Total samples: 2394
- soil counts: 432
- aquatic counts: 339
- animal counts: 1469
- plant counts: 154
Safety information
risk assessment
- @ref: 6650
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Ochrobactrum lupini gene for 16S rRNA, partial sequence, strain: NBRC 102587 | AB681867 | 1408 | ena | 255457 |
| 6650 | Ochrobactrum lupini 16S ribosomal RNA gene, partial sequence | AY457038 | 1477 | ena | 255457 |
GC content
- @ref: 6650
- GC-content: 56.8
External links
@ref: 6650
culture collection no.: CCUG 54647, DSM 16930, LMG 22726, NBRC 102587, Lup LUP21, Lup 21
straininfo link
- @ref: 71533
- straininfo: 133210
literature
- topic: Phylogeny
- Pubmed-ID: 26615404
- title: Ochrobactrum endophyticum sp. nov., isolated from roots of Glycyrrhiza uralensis.
- authors: Li L, Li YQ, Jiang Z, Gao R, Nimaichand S, Duan YQ, Egamberdieva D, Chen W, Li WJ
- journal: Arch Microbiol
- DOI: 10.1007/s00203-015-1170-8
- year: 2015
- mesh: Base Composition, China, Fatty Acids/analysis, Glycyrrhiza uralensis/*microbiology, Ochrobactrum/*classification/genetics/isolation & purification, Phospholipids/analysis, *Phylogeny, Plant Roots/*microbiology, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity
- topic2: Transcriptome
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 6650 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16930) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16930 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 60280 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 54647) | https://www.ccug.se/strain?id=54647 | |||
| 68369 | Automatically annotated from API 20NE | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 71533 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID133210.1 | StrainInfo: A central database for resolving microbial strain identifiers |