Strain identifier

BacDive ID: 188

Type strain: Yes

Species: Bowdeniella nasicola

Strain Designation: R2014

Strain history: CIP <- 2002, CCUG <- Val Hall UK, Cardiff, PHLS: strain R2014

NCBI tax ID(s): 208480 (species)

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General

@ref: 7915

BacDive-ID: 188

DSM-Number: 19116

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Bowdeniella nasicola R2014 is an anaerobe, mesophilic, Gram-positive human pathogen that was isolated from human anthral washout.

NCBI tax id

  • NCBI tax id: 208480
  • Matching level: species

strain history

@refhistory
7915<- CCUG <- V. Hall, R2014
121236CIP <- 2002, CCUG <- Val Hall UK, Cardiff, PHLS: strain R2014

doi: 10.13145/bacdive188.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Bowdeniella
  • species: Bowdeniella nasicola
  • full scientific name: Bowdeniella nasicola corrig. (Hall et al. 2003) Nouioui et al. 2018

  • synonyms

    @refsynonym
    20215Bowdenia nasicola
    20215Actinomyces nasicola

@ref: 7915

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Bowdeniella

species: Bowdeniella nasicola

full scientific name: Bowdeniella nasicola (Hall et al. 2003) Nouioui et al. 2018

strain designation: R2014

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
23122positiverod-shaped
69480no90.624
69480positive100
121236positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium usedhemolysis ability
23122white or grey2 daysfastidious anaerobe agar
1212361

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7915PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
23122fastidious anaerobe agaryes
33855MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
121236CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
7915positivegrowth37mesophilic
33855positivegrowth37mesophilic
57055positivegrowth37mesophilic
121236positivegrowth25-41
121236nogrowth10psychrophilic
121236nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7915anaerobe
23122facultative anaerobe
57055microaerophile
121236facultative anaerobe

spore formation

@refspore formationconfidence
23122no
69481no100
69480no99.995

halophily

@refsaltgrowthtested relationconcentration
121236NaClpositivegrowth0-4 %
121236NaClnogrowth6 %
121236NaClnogrowth8 %
121236NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2312227613amygdalin-builds acid from
2312222599arabinose-builds acid from
2312223456cyclodextrin-builds acid from
2312218333D-arabitol-builds acid from
2312265327D-xylose-builds acid from
2312217234glucose-builds acid from
2312228087glycogen-builds acid from
2312230849L-arabinose-builds acid from
2312217716lactose-builds acid from
2312217306maltose-builds acid from
2312229864mannitol-builds acid from
2312237684mannose-builds acid from
231226731melezitose-builds acid from
2312228053melibiose-builds acid from
23122320055methyl beta-D-glucopyranoside-builds acid from
2312227941pullulan-builds acid from
2312216634raffinose-builds acid from
2312233942ribose-builds acid from
2312217814salicin-builds acid from
2312230911sorbitol-builds acid from
2312217992sucrose-builds acid from
2312233954tagatose-builds acid from
2312227082trehalose-builds acid from
2312218222xylose-builds acid from
2312227897tryptophan-energy source
231224853esculin-hydrolysis
231225291gelatin-hydrolysis
23122606565hippurate-hydrolysis
2312228017starch-hydrolysis
2312217632nitrate-reduction
2312217057cellobiose+builds acid from
2312228757fructose+builds acid from
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose+builds acid from
6837117814salicin-builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12123616947citrate-carbon source
1212364853esculin+hydrolysis
121236606565hippurate+hydrolysis
12123617632nitrate-reduction
12123616301nitrite-reduction
12123617632nitrate+respiration
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016634raffinose-fermentation
6838029985L-glutamate+degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
2312235581indoleno
2312215688acetoinno
12123635581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
2312215688acetoin-
2312235581indole-
6838035581indole-
12123615688acetoin-
12123617234glucose-

enzymes

@refvalueactivityec
231226-phospho-beta-galactosidase+3.2.1.85
231226-phospho-beta-galactosidase-3.2.1.85
23122acid phosphatase-3.1.3.2
23122alanine arylamidase+3.4.11.2
23122alkaline phosphatase-3.1.3.1
23122alpha-arabinosidase-3.2.1.55
23122alpha-fucosidase-3.2.1.51
23122alpha-galactosidase-3.2.1.22
23122alpha-glucosidase+3.2.1.20
23122alpha-mannosidase-3.2.1.24
23122arginine dihydrolase+/-3.5.3.6
23122beta-glucosidase+/-3.2.1.21
23122beta-glucuronidase-3.2.1.31
23122beta-mannosidase-3.2.1.25
23122catalase-1.11.1.6
23122chymotrypsin-3.4.4.5
23122esterase (C 4)-
23122esterase lipase (C 8)-
23122glutamate decarboxylase-4.1.1.15
23122glutamyl-glutamate arylamidase+/-
23122glycin arylamidase+
23122glycyl tryptophan arylamidase-
23122histidine arylamidase+
23122L-arginine arylamidase+
23122lecithinase-
23122leucine arylamidase+3.4.11.1
23122lipase-
23122lipase (C 14)-
23122N-acetyl-beta-glucosaminidase+3.2.1.52
23122phosphoamidase-3.9.1.1
23122proline-arylamidase+3.4.11.5
23122pyroglutamic acid arylamidase+/-
23122pyrrolidonyl arylamidase-3.4.19.3
23122serine arylamidase+
23122tripeptide aminopeptidase+3.4.11.4
23122trypsin-3.4.21.4
23122tryptophan deaminase-4.1.99.1
23122tyrosine arylamidase+
23122urease-3.5.1.5
23122valine arylamidase+
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68380glutamyl-glutamate arylamidase-
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase+4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
121236oxidase+
121236beta-galactosidase+3.2.1.23
121236alcohol dehydrogenase-1.1.1.1
121236gelatinase-
121236amylase+
121236DNase-
121236caseinase-3.4.21.50
121236catalase-1.11.1.6
121236tween esterase+
121236gamma-glutamyltransferase-2.3.2.2
121236lecithinase-
121236lipase-
121236lysine decarboxylase-4.1.1.18
121236ornithine decarboxylase-4.1.1.17
121236phenylalanine ammonia-lyase-4.3.1.24
121236protease-
121236tryptophan deaminase-
121236urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121236--++-++---++-+-++++-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121236------------+/-----------++/--+--+/------+---+---------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
7915---+-++--++/--+----++-++-++++/--+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
7915human anthral washoutCardiff; PHLSUnited KingdomGBREurope
2312281-year-old male patient admitted for routine nasal polypectomy
57055Human antral washout,81-yr-old maleLiverpoolUnited KingdomGBREurope1985
121236Human, Antral washoutLiverpoolUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host#Human#Male

taxonmaps

  • @ref: 69479
  • File name: preview.99_6378.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_224;96_3027;97_3718;98_4713;99_6378&stattab=map
  • Last taxonomy: Bowdeniella nasicola subclade
  • 16S sequence: AJ508455
  • Sequence Identity:
  • Total samples: 2815
  • soil counts: 525
  • aquatic counts: 314
  • animal counts: 1884
  • plant counts: 92

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
7915yes, in single cases1Risk group (German classification)
1212362Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7915
  • description: Actinomyces nasicol partial 16S rRNA gene, strain CCUG 46092
  • accession: AJ508455
  • length: 1475
  • database: ena
  • NCBI tax ID: 208480

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bowdeniella nasicola DSM 19116GCA_001907295scaffoldncbi208480
66792Actinomyces nasicola strain DSM 19116208480.4wgspatric208480
66792Actinomyces nasicola DSM 191162855319728draftimg208480

GC content

@refGC-contentmethod
791566.5
2312266.5high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno97.976no
gram-positiveyes92.467yes
anaerobicno93.302no
halophileno79.05no
spore-formingno95.011yes
thermophileno94.944yes
glucose-utilyes83.714no
aerobicno82.134yes
motileno91.302no
glucose-fermentno55.672no

External links

@ref: 7915

culture collection no.: DSM 19116, CCUG 46092, CIP 107668

straininfo link

  • @ref: 69870
  • straininfo: 97622

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130031Actinomyces nasicola sp. nov., isolated from a human nose.Hall V, Collins MD, Lawson PA, Falsen E, Duerden BIInt J Syst Evol Microbiol10.1099/ijs.0.02582-02003Actinomyces/classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Humans, Molecular Sequence Data, Nose/*microbiology, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/geneticsPathogenicity
Phylogeny34431769Nanchangia anserum gen. nov., sp. nov., isolated from feces of greater white-fronted geese (Anser albifrons).Liu Q, Xue L, Wu K, Fan G, Bai X, Yang X, Cao L, Sun H, Song W, Pan Y, Chen S, Xiong Y, Chen HInt J Syst Evol Microbiol10.1099/ijsem.0.0049782021Actinomycetaceae/*classification/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, *Geese/microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7915Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19116)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19116
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23122Val Hall, Matthew D. Collins, Paul A. Lawson, Enevold Falsen, Brian I. Duerden10.1099/ijs.0.02582-0Actinomyces nasicola sp. nov., isolated from a human noseIJSEM 53: 1445-1448 200313130031
33855Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5129
57055Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46092)https://www.ccug.se/strain?id=46092
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69870Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID97622.1StrainInfo: A central database for resolving microbial strain identifiers
121236Curators of the CIPCollection of Institut Pasteur (CIP 107668)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107668