Strain identifier
BacDive ID: 1878
Type strain:
Species: Brucella anthropi
Strain history: IAM 14119 <-- LMG 3331 <-- M. Popoff ALC 037 <-- CIP 149.70.
NCBI tax ID(s): 529 (species)
General
@ref: 2871
BacDive-ID: 1878
DSM-Number: 6882
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, chemoorganotroph, mesophilic, Gram-negative, motile, rod-shaped, human pathogen
description: Brucella anthropi DSM 6882 is an obligate aerobe, chemoorganotroph, mesophilic human pathogen that was isolated from human clinical specimen.
NCBI tax id
- NCBI tax id: 529
- Matching level: species
strain history
@ref | history |
---|---|
2871 | <- LMG; LMG 3331 <- M. Popoff, IP; CIP 82.115 (Alcaligenes denitrificans) <- CIP; CIP 149-70 |
39032 | 1982, M. Popoff, Inst. Pasteur, Paris, France: strain ALC 037, Achromobacter sp., group Vd |
67770 | IAM 14119 <-- LMG 3331 <-- M. Popoff ALC 037 <-- CIP 149.70. |
doi: 10.13145/bacdive1878.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Brucellaceae
- genus: Brucella
- species: Brucella anthropi
- full scientific name: Brucella anthropi (Holmes et al. 1988) Hördt et al. 2020
synonyms
- @ref: 20215
- synonym: Ochrobactrum anthropi
@ref: 2871
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Brucellaceae
genus: Ochrobactrum
species: Ochrobactrum anthropi
full scientific name: Ochrobactrum anthropi Holmes et al. 1988
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell shape | motility | flagellum arrangement | confidence |
---|---|---|---|---|---|
23334 | negative | rod-shaped | yes | peritrichous | |
69480 | yes | 92.826 | |||
69480 | negative | 99.993 |
colony morphology
@ref | incubation period | colony size | colony shape | medium used |
---|---|---|---|---|
2871 | 1-2 days | |||
23334 | 1 day | 1 mm | circular | nutrient agar |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
2871 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
2871 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | https://mediadive.dsmz.de/medium/220 | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water |
23334 | MacConkey | yes | ||
23334 | Nutrient agar (NA) | yes | ||
39032 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
2871 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
2871 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
2871 | positive | growth | 25 | mesophilic |
23334 | no | growth | 42 | thermophilic |
23334 | no | growth | 5 | psychrophilic |
23334 | positive | optimum | 20-37 | |
39032 | positive | growth | 30 | mesophilic |
2871 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 23334
- oxygen tolerance: obligate aerobe
nutrition type
- @ref: 23334
- type: chemoorganotroph
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.998 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23334 | 32800 | (S)-mandelic acid | - | assimilation |
23334 | 16567 | 2-aminobenzoate | - | assimilation |
23334 | 28340 | 2-aminobutyrate | - | assimilation |
23334 | 16193 | 3-hydroxybenzoate | - | assimilation |
23334 | 22210 | aconitate | - | assimilation |
23334 | 17128 | adipate | - | assimilation |
23334 | 18305 | arbutin | - | assimilation |
23334 | 78208 | azelaate | - | assimilation |
23334 | 16150 | benzoate | - | assimilation |
23334 | 27689 | decanoate | - | assimilation |
23334 | 30719 | citraconate | - | assimilation |
23334 | 16919 | creatine | - | assimilation |
23334 | 6731 | melezitose | - | assimilation |
23334 | 28053 | melibiose | - | assimilation |
23334 | 16634 | raffinose | - | assimilation |
23334 | 30927 | D-tartrate | - | assimilation |
23334 | 4853 | esculin | - | assimilation |
23334 | 16028 | L-2,4-diaminobutyrate | - | assimilation |
23334 | 18403 | L-arabitol | - | assimilation |
23334 | 17561 | L-cysteine | - | assimilation |
23334 | 16643 | L-methionine | - | assimilation |
23334 | 17295 | L-phenylalanine | - | assimilation |
23334 | 17266 | L-sorbose | - | assimilation |
23334 | 16828 | L-tryptophan | - | assimilation |
23334 | 17895 | L-tyrosine | - | assimilation |
23334 | 65328 | L-xylose | - | assimilation |
23334 | 17716 | lactose | - | assimilation |
23334 | 18300 | maleic acid | - | assimilation |
23334 | 15792 | malonate | - | assimilation |
23334 | 30928 | meso-tartrate | - | assimilation |
23334 | 43943 | methyl alpha-D-mannoside | - | assimilation |
23334 | 32361 | nonanoate | - | assimilation |
23334 | 30623 | oxalate | - | assimilation |
23334 | 18401 | phenylacetate | - | assimilation |
23334 | 17814 | salicin | - | assimilation |
23334 | 41865 | sebacic acid | - | assimilation |
23334 | 28017 | starch | - | assimilation |
23334 | 9300 | suberic acid | - | assimilation |
23334 | 18123 | trigonelline | - | assimilation |
23334 | 17151 | xylitol | - | assimilation |
23334 | 17716 | lactose | - | builds acid from |
23334 | 16634 | raffinose | - | builds acid from |
23334 | 17814 | salicin | - | builds acid from |
23334 | 16947 | citrate | - | carbon source |
23334 | casein | - | degradation | |
23334 | 28044 | phenylalanine | - | degradation |
23334 | 27442 | beta-D-fucose | - | hydrolysis |
23334 | 16991 | dna | - | hydrolysis |
23334 | 4853 | esculin | - | hydrolysis |
23334 | 5291 | gelatin | - | hydrolysis |
23334 | 320061 | methyl alpha-D-glucopyranoside | - | hydrolysis |
23334 | 17540 | methyl beta-D-galactoside | - | hydrolysis |
23334 | 28017 | starch | - | hydrolysis |
23334 | 53424 | tween 20 | - | hydrolysis |
23334 | 53426 | tween 80 | - | hydrolysis |
23334 | 18186 | tyrosine | - | hydrolysis |
23334 | 19475 | 2-aminopentanoic acid | + | assimilation |
23334 | 2-oxogluconate | + | assimilation | |
23334 | 30916 | 2-oxoglutarate | + | assimilation |
23334 | 87997 | 3-aminobutyrate | + | assimilation |
23334 | 37054 | 3-hydroxybutyrate | + | assimilation |
23334 | 17879 | 4-hydroxybenzoate | + | assimilation |
23334 | 15887 | 5-aminovaleric acid | + | assimilation |
23334 | 17426 | 5-dehydro-D-gluconate | + | assimilation |
23334 | 15963 | ribitol | + | assimilation |
23334 | 27613 | amygdalin | + | assimilation |
23334 | 16958 | beta-alanine | + | assimilation |
23334 | 17750 | betaine | + | assimilation |
23334 | 17968 | butyrate | + | assimilation |
23334 | 27689 | decanoate | + | assimilation |
23334 | 16947 | citrate | + | assimilation |
23334 | 15570 | D-alanine | + | assimilation |
23334 | 17108 | D-arabinose | + | assimilation |
23334 | 18333 | D-arabitol | + | assimilation |
23334 | 17057 | cellobiose | + | assimilation |
23334 | 28847 | D-fucose | + | assimilation |
23334 | 62318 | D-lyxose | + | assimilation |
23334 | 15588 | D-malate | + | assimilation |
23334 | 16443 | D-tagatose | + | assimilation |
23334 | 32528 | turanose | + | assimilation |
23334 | 65327 | D-xylose | + | assimilation |
23334 | 16813 | galactitol | + | assimilation |
23334 | 17113 | erythritol | + | assimilation |
23334 | 16000 | ethanolamine | + | assimilation |
23334 | 29806 | fumarate | + | assimilation |
23334 | 28260 | galactose | + | assimilation |
23334 | 28066 | gentiobiose | + | assimilation |
23334 | 24265 | gluconate | + | assimilation |
23334 | 17859 | glutaric acid | + | assimilation |
23334 | 17754 | glycerol | + | assimilation |
23334 | 15428 | glycine | + | assimilation |
23334 | 29805 | glycolate | + | assimilation |
23334 | 17268 | myo-inositol | + | assimilation |
23334 | 48944 | isobutyrate | + | assimilation |
23334 | 48942 | isovalerate | + | assimilation |
23334 | 30849 | L-arabinose | + | assimilation |
23334 | 16467 | L-arginine | + | assimilation |
23334 | 29991 | L-aspartate | + | assimilation |
23334 | 16349 | L-citrulline | + | assimilation |
23334 | 17191 | L-isoleucine | + | assimilation |
23334 | 15603 | L-leucine | + | assimilation |
23334 | 18019 | L-lysine | + | assimilation |
23334 | 15589 | L-malate | + | assimilation |
23334 | 18347 | L-norleucine | + | assimilation |
23334 | 15729 | L-ornithine | + | assimilation |
23334 | 17115 | L-serine | + | assimilation |
23334 | 16414 | L-valine | + | assimilation |
23334 | 17306 | maltose | + | assimilation |
23334 | 29864 | mannitol | + | assimilation |
23334 | 506227 | N-acetylglucosamine | + | assimilation |
23334 | 17272 | propionate | + | assimilation |
23334 | 15361 | pyruvate | + | assimilation |
23334 | 26546 | rhamnose | + | assimilation |
23334 | 33942 | ribose | + | assimilation |
23334 | 15611 | sarcosine | + | assimilation |
23334 | 30911 | sorbitol | + | assimilation |
23334 | 30031 | succinate | + | assimilation |
23334 | 17992 | sucrose | + | assimilation |
23334 | 27082 | trehalose | + | assimilation |
23334 | 31011 | valerate | + | assimilation |
23334 | 15963 | ribitol | + | builds acid from |
23334 | 22599 | arabinose | + | builds acid from |
23334 | 17057 | cellobiose | + | builds acid from |
23334 | 16236 | ethanol | + | builds acid from |
23334 | 28757 | fructose | + | builds acid from |
23334 | 17234 | glucose | + | builds acid from |
23334 | 17754 | glycerol | + | builds acid from |
23334 | 17268 | myo-inositol | + | builds acid from |
23334 | 17306 | maltose | + | builds acid from |
23334 | 29864 | mannitol | + | builds acid from |
23334 | 26546 | rhamnose | + | builds acid from |
23334 | 30911 | sorbitol | + | builds acid from |
23334 | 17992 | sucrose | + | builds acid from |
23334 | 27082 | trehalose | + | builds acid from |
23334 | 18222 | xylose | + | builds acid from |
23334 | 35020 | tributyrin | + | hydrolysis |
23334 | 17632 | nitrate | + | reduction |
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 17306 | maltose | + | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 30849 | L-arabinose | + | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | + | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | + | reduction |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive |
---|---|---|---|---|
23334 | 3393 | carbenicillin | yes | yes |
23334 | 3770 | co-trimoxazole | yes | yes |
23334 | 100147 | nalidixic acid | yes | yes |
23334 | 27902 | tetracycline | yes | yes |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
23334 | 53388 | polyhydroxybutyrate | no |
23334 | 35581 | indole | no |
23334 | 16136 | hydrogen sulfide | no |
68369 | 35581 | indole | no |
metabolite tests
@ref | Chebi-ID | metabolite | citrate test | indole test |
---|---|---|---|---|
23334 | 16947 | citrate | - | |
68369 | 35581 | indole | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
23334 | alpha-chymotrypsin | - | 3.4.21.1 |
23334 | alpha-fucosidase | - | 3.2.1.51 |
23334 | alpha-galactosidase | - | 3.2.1.22 |
23334 | alpha-glucosidase | - | 3.2.1.20 |
23334 | alpha-mannosidase | - | 3.2.1.24 |
23334 | arginine dihydrolase | - | 3.5.3.6 |
23334 | beta-galactosidase | - | 3.2.1.23 |
23334 | beta-glucosidase | - | 3.2.1.21 |
23334 | beta-glucuronidase | - | 3.2.1.31 |
23334 | catalase | + | 1.11.1.6 |
23334 | cystine arylamidase | - | 3.4.11.3 |
23334 | cytochrome oxidase | + | 1.9.3.1 |
23334 | esterase lipase (C 8) | - | |
23334 | gelatinase | - | |
23334 | lecithinase | - | |
23334 | lipase (C 14) | - | |
23334 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
23334 | naphthol-AS-BI-phosphohydrolase | - | |
23334 | phosphatase | - | |
23334 | urease | + | 3.5.1.5 |
23334 | valine arylamidase | - | |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | + | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2871 | + | - | - | - | + | - | - | - | + | + | + | + | + | + | + | + | - | + | + | - | + |
2871 | + | - | - | - | + | - | - | - | + | + | - | - | + | + | - | - | - | + | +/- | - | +/- |
2871 | + | - | - | - | + | - | - | - | + | + | + | +/- | + | + | +/- | + | - | + | +/- | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
23334 | human clinical specimen |
2871 | human clinical specimen |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Human | |
#Infection | #Patient | |
#Infection | #Medical environment | #Clinic |
#Infection | #Patient | #Specimen |
taxonmaps
- @ref: 69479
- File name: preview.99_2226.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_45;97_1426;98_1736;99_2226&stattab=map
- Last taxonomy: Ochrobactrum
- 16S sequence: AM114398
- Sequence Identity:
- Total samples: 2394
- soil counts: 432
- aquatic counts: 339
- animal counts: 1469
- plant counts: 154
Safety information
risk assessment
- @ref: 2871
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Ochrobactrum anthropi 16S rRNA gene (partial), 23S rRNA gene (partial), tRNA-Ile gene, tRNA-Ala gene and ITS1, type strain LMG 3331 | AJ867295 | 880 | ena | 439375 |
20218 | Ochrobactrum anthropi strain STM 2148 16S ribosomal RNA gene, partial sequence | AY785314 | 1401 | ena | 529 |
20218 | Ochrobactrum anthropi ATCC:49188 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | FJ410394 | 3617 | ena | 439375 |
20218 | O.anthrois internal transcribed spacer region between 16S and 23S rRNA | X95892 | 706 | ena | 529 |
2871 | Ochrobactrum anthropi partial 16S rRNA gene, type strain LMG 3331T | AM114398 | 1388 | ena | 439375 |
67770 | Ochrobactrum anthropi gene for 16S rRNA, strain: IAM 14119 | D12794 | 1436 | ena | 529 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Brucella anthropi ATCC 49188 | GCA_000017405 | complete | ncbi | 439375 |
66792 | Ochrobactrum anthropi ATCC 49188 | 439375.63 | wgs | patric | 439375 |
66792 | Ochrobactrum anthropi ATCC 49188 | 439375.59 | plasmid | patric | 439375 |
66792 | Ochrobactrum anthropi ATCC 49188 | 439375.57 | plasmid | patric | 439375 |
66792 | Ochrobactrum anthropi ATCC 49188 | 439375.58 | plasmid | patric | 439375 |
66792 | Ochrobactrum anthropi ATCC 49188 | 439375.60 | plasmid | patric | 439375 |
66792 | Ochrobactrum anthropi ATCC 49188 | 439375.7 | complete | patric | 439375 |
66792 | Ochrobactrum anthropi strain NCTC12168 | 529.64 | wgs | patric | 529 |
66792 | Brucella anthropi ATCC 49188 | 640753038 | complete | img | 439375 |
67770 | Brucella anthropi ATCC 49188 | GCA_012103185 | contig | ncbi | 439375 |
67770 | Brucella anthropi NCTC12168 | GCA_900454235 | contig | ncbi | 529 |
GC content
@ref | GC-content | method |
---|---|---|
2871 | 57.2 | |
23334 | 57.00 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | yes | 84.486 | no |
flagellated | no | 83.499 | no |
gram-positive | no | 98.453 | yes |
anaerobic | no | 97.707 | no |
halophile | no | 93.156 | no |
spore-forming | no | 96.139 | no |
thermophile | no | 98.769 | no |
glucose-util | yes | 93.881 | no |
aerobic | yes | 89.496 | yes |
glucose-ferment | no | 90.612 | yes |
External links
@ref: 2871
culture collection no.: DSM 6882, Alc 37, ATCC 49188, CIP 149-70, CIP 82.115, LMG 3331, JCM 21032, CCUG 24695, CIP 149.70, IAM 14119, IFO 15819, NBRC 15819, NCTC 12168
straininfo link
- @ref: 71523
- straininfo: 88044
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
2871 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6882) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-6882 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
23334 | B. HOLMES, M. POPOFF, M. KIREDJIAN, K. KERSTERS | 10.1099/00207713-38-4-406 | Ochrobactrum anthropi gen. nov., sp. nov. from Human Clinical Specimens and Previously Known as Group Vd | IJSEM 38: 406-416 1988 | ||
39032 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/11393 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68369 | Automatically annotated from API 20NE | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
71523 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID88044.1 | StrainInfo: A central database for resolving microbial strain identifiers |