Strain identifier
BacDive ID: 1860
Type strain:
Species: Brevibacterium picturae
Strain Designation: R-5391
Strain history: CIP <- 2005, DSMZ <- J. Heyrman, Ghent Univ., Ghent, Belgium: strain R-5391
NCBI tax ID(s): 260553 (species)
General
@ref: 6306
BacDive-ID: 1860
DSM-Number: 16132
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped
description: Brevibacterium picturae R-5391 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from biofilm covering mural painting.
NCBI tax id
- NCBI tax id: 260553
- Matching level: species
strain history
@ref | history |
---|---|
6306 | <- J. Heyrman; R-5391 |
67770 | LMG 22061 <-- J. Heyrman R-5391. |
121822 | CIP <- 2005, DSMZ <- J. Heyrman, Ghent Univ., Ghent, Belgium: strain R-5391 |
doi: 10.13145/bacdive1860.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Brevibacteriaceae
- genus: Brevibacterium
- species: Brevibacterium picturae
- full scientific name: Brevibacterium picturae Heyrman et al. 2004
@ref: 6306
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Brevibacteriaceae
genus: Brevibacterium
species: Brevibacterium picturae
full scientific name: Brevibacterium picturae Heyrman et al. 2004
strain designation: R-5391
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
31255 | positive | 2.75 µm | 1 µm | coccus-shaped | no |
121822 | positive | rod-shaped | no |
colony morphology
- @ref: 121822
pigmentation
- @ref: 31255
- production: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
6306 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
37575 | Marine agar (MA) | yes | Distilled water make up to (1000.000 ml);Marine agar (55.100 g) | |
121822 | CIP Medium 13 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13 | |
121822 | CIP Medium 338 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=338 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
6306 | positive | growth | 28 | mesophilic |
31255 | positive | growth | 15-37 | |
31255 | positive | optimum | 25 | mesophilic |
37575 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
121822 | positive | growth | 10-30 | |
121822 | no | growth | 37 | mesophilic |
121822 | no | growth | 41 | thermophilic |
121822 | no | growth | 45 | thermophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
31255 | positive | growth | 06-09 | alkaliphile |
31255 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
31255 | aerobe |
121822 | obligate aerobe |
spore formation
- @ref: 31255
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
31255 | NaCl | positive | growth | 05-15 % |
31255 | NaCl | positive | optimum | 10 % |
121822 | NaCl | positive | growth | 2-10 % |
121822 | NaCl | no | growth | 0 % |
observation
- @ref: 67770
- observation: quinones: MK-8(H2)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31255 | 17234 | glucose | + | carbon source |
121822 | 16947 | citrate | + | carbon source |
121822 | 4853 | esculin | - | hydrolysis |
121822 | 606565 | hippurate | - | hydrolysis |
121822 | 17632 | nitrate | - | reduction |
121822 | 16301 | nitrite | - | reduction |
121822 | 17632 | nitrate | - | respiration |
metabolite production
- @ref: 121822
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
121822 | 15688 | acetoin | - | |
121822 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
31255 | acid phosphatase | + | 3.1.3.2 |
31255 | alkaline phosphatase | + | 3.1.3.1 |
31255 | catalase | + | 1.11.1.6 |
31255 | gelatinase | + | |
31255 | pyrazinamidase | + | 3.5.1.B15 |
31255 | urease | + | 3.5.1.5 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
121822 | oxidase | - | |
121822 | beta-galactosidase | - | 3.2.1.23 |
121822 | alcohol dehydrogenase | - | 1.1.1.1 |
121822 | gelatinase | + | |
121822 | amylase | - | |
121822 | DNase | - | |
121822 | caseinase | + | 3.4.21.50 |
121822 | catalase | + | 1.11.1.6 |
121822 | tween esterase | - | |
121822 | gamma-glutamyltransferase | + | 2.3.2.2 |
121822 | lecithinase | - | |
121822 | lipase | - | |
121822 | lysine decarboxylase | - | 4.1.1.18 |
121822 | ornithine decarboxylase | - | 4.1.1.17 |
121822 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
121822 | tryptophan deaminase | - | |
121822 | urease | + | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121822 | - | + | + | + | - | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121822 | + | - | + | - | + | + | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | + | - | - | - | + | - | - | - | - | - | - | + | + | + | + | + | - | - | - | - | + | + | + | + | + | - | + | - | - | + | + | + | + | + | + | - | - | + | + | + | + | + | + | + | + | + | + | + | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
6306 | biofilm covering mural painting | Styria | Austria | AUT | Europe |
67770 | Damaged mural painting at the Saint-Catherine chapel of Castle Herberstein | Austria | AUT | Europe | |
121822 | Covering mural painting | Austria | AUT | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Other | #Painting |
#Environmental | #Biofilm |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
6306 | 1 | Risk group (German classification) |
121822 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 6306
- description: Brevibacterium picturae partial 16S rRNA gene, type strain LMG 22061T
- accession: AJ620364
- length: 1493
- database: ena
- NCBI tax ID: 260553
GC content
@ref | GC-content | method |
---|---|---|
6306 | 63.3 | |
67770 | 63.3 | high performance liquid chromatography (HPLC) |
External links
@ref: 6306
culture collection no.: DSM 16132, CIP 108823, LMG 22061, JCM 13319
straininfo link
- @ref: 71505
- straininfo: 128654
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 15388707 | Brevibacterium picturae sp. nov., isolated from a damaged mural painting at the Saint-Catherine chapel (Castle Herberstein, Austria). | Heyrman J, Verbeeren J, Schumann P, Devos J, Swings J, De Vos P | Int J Syst Evol Microbiol | 10.1099/ijs.0.63144-0 | 2004 | Austria, Base Composition, Brevibacterium/chemistry/*classification/genetics/*isolation & purification, Cardiolipins/analysis, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Diaminopimelic Acid/analysis, Enzymes/analysis, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Mycolic Acids/analysis, *Paintings, Peptidoglycan/analysis, Phosphatidylglycerols/analysis, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analysis | Enzymology |
Phylogeny | 18218957 | Brevibacterium marinum sp. nov., isolated from seawater. | Lee SD | Int J Syst Evol Microbiol | 10.1099/ijs.0.65099-0 | 2008 | Bacterial Typing Techniques, Base Composition, Brevibacterium/*classification/genetics/*isolation & purification/physiology, DNA, Bacterial/analysis, Fatty Acids/analysis, Genes, rRNA, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Species Specificity | Genetics |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
6306 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16132) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16132 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
31255 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27578 | 28776041 | |
37575 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/6494 | ||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68382 | Automatically annotated from API zym | |||||
71505 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID128654.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121822 | Curators of the CIP | Collection of Institut Pasteur (CIP 108823) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108823 |