Strain identifier

BacDive ID: 186

Type strain: Yes

Species: Actinomyces graevenitzii

Strain history: CIP <- 1998, CCUG <- Res. Dev., PHLS, Göteborg, Sweden

NCBI tax ID(s): 55565 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.3 (current version):
version 9.2:
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.3 (current version)

General

@ref: 5989

BacDive-ID: 186

DSM-Number: 15540

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Actinomyces graevenitzii DSM 15540 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from human bronchus brush, 57-yr-old woman.

NCBI tax id

  • NCBI tax id: 55565
  • Matching level: species

strain history

@refhistory
5989<- CCUG <- PHLS, Sweden
119721CIP <- 1998, CCUG <- Res. Dev., PHLS, Göteborg, Sweden

doi: 10.13145/bacdive186.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Actinomyces
  • species: Actinomyces graevenitzii
  • full scientific name: Actinomyces graevenitzii Pascual Ramos et al. 1997

@ref: 5989

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Actinomyces

species: Actinomyces graevenitzii

full scientific name: Actinomyces graevenitzii Pascual Ramos et al. 1997

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
23120positiverod-shapedno
119721positiverod-shapedno

colony morphology

@refcolony sizeincubation periodmedium usedhemolysis ability
231200.2 mm1 dayblood agar
487022-3 days
1197211

pigmentation

  • @ref: 23120
  • production: no
  • name: no pigments

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5989FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)yeshttps://mediadive.dsmz.de/medium/1203Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water
5989COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
23120blood agaryes
39350MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
119721CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperature
5989positivegrowth37
39350positivegrowth37
48702positivegrowth37
119721positivegrowth25-41
119721nogrowth10
119721nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23120facultative anaerobe
48702microaerophile
119721facultative anaerobe

spore formation

  • @ref: 23120
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
119721NaClpositivegrowth0-2 %
119721NaClnogrowth4 %
119721NaClnogrowth6 %
119721NaClnogrowth8 %
119721NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2312027613amygdalin-builds acid from
2312017057cellobiose-builds acid from
2312017108D-arabinose-builds acid from
2312018333D-arabitol-builds acid from
2312028847D-fucose-builds acid from
2312065327D-xylose-builds acid from
2312016813galactitol-builds acid from
2312017113erythritol-builds acid from
2312028066gentiobiose-builds acid from
2312028087glycogen-builds acid from
2312015443inulin-builds acid from
2312018403L-arabitol-builds acid from
2312018287L-fucose-builds acid from
2312017266L-sorbose-builds acid from
2312065328L-xylose-builds acid from
2312029864mannitol-builds acid from
231206731melezitose-builds acid from
2312028053melibiose-builds acid from
2312074863methyl beta-D-xylopyranoside-builds acid from
2312037657methyl D-glucoside-builds acid from
2312026546rhamnose-builds acid from
2312017814salicin-builds acid from
2312030911sorbitol-builds acid from
2312028017starch-builds acid from
2312027082trehalose-builds acid from
2312017151xylitol-builds acid from
231204853esculin-hydrolysis
23120606565hippurate-hydrolysis
2312016199urea-hydrolysis
2312017632nitrate-reduction
23120174265-dehydro-D-gluconate+builds acid from
2312016443D-tagatose+builds acid from
2312028757fructose+builds acid from
2312028260galactose+builds acid from
2312017754glycerol+builds acid from
2312017716lactose+builds acid from
2312017306maltose+builds acid from
2312017268myo-inositol+builds acid from
2312028037N-acetylgalactosamine+builds acid from
2312033942ribose+builds acid from
2312017992sucrose+builds acid from
6837727897tryptophan-energy source
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837717306maltose+builds acid from
6837715824D-fructose+builds acid from
6837717634D-glucose+builds acid from
6837928087glycogen-fermentation
6837917716lactose+fermentation
6837917306maltose+fermentation
6837916899D-mannitol-fermentation
6837916199urea-hydrolysis
683794853esculin-hydrolysis
6837917632nitrate-reduction
11972116947citrate-carbon source
1197214853esculin-hydrolysis
119721606565hippurate-hydrolysis
11972117632nitrate-reduction
11972116301nitrite-reduction
11972117632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837717992sucrose+builds acid from
6837917992sucrose+fermentation
6837965327D-xylose-fermentation
6837917634D-glucose+fermentation
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from

antibiotic resistance

  • @ref: 119721
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
11972135581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6837735581indole-
11972115688acetoin-
11972117234glucose+

enzymes

@refvalueactivityec
23120acid phosphatase+3.1.3.2
23120alpha-fucosidase-3.2.1.51
23120alpha-galactosidase-3.2.1.22
23120alpha-glucosidase-3.2.1.20
23120alpha-mannosidase-3.2.1.24
23120beta-galactosidase+3.2.1.23
23120beta-glucosidase-3.2.1.21
23120beta-glucuronidase-3.2.1.31
23120chymotrypsin-3.4.4.5
23120leucine arylamidase+3.4.11.1
23120lipase-
23120N-acetyl-beta-glucosaminidase+3.2.1.52
23120phosphoamidase+3.9.1.1
23120pyrrolidonyl arylamidase-3.4.19.3
23120trypsin-3.4.21.4
23120valine arylamidase-
68382alpha-mannosidase-3.2.1.24
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382valine arylamidase-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
119721oxidase-
119721beta-galactosidase+3.2.1.23
119721alcohol dehydrogenase-1.1.1.1
119721gelatinase+/-
119721amylase+
119721DNase-
119721caseinase-3.4.21.50
119721catalase-1.11.1.6
119721tween esterase-
119721gamma-glutamyltransferase-2.3.2.2
119721lecithinase-
119721lipase-
119721lysine decarboxylase-4.1.1.18
119721ornithine decarboxylase-4.1.1.17
119721phenylalanine ammonia-lyase-4.3.1.24
119721protease-
119721tryptophan deaminase-
119721urease-3.5.1.5
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68379beta-glucosidase-3.2.1.21
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68382alpha-fucosidase-3.2.1.51
68382alpha-glucosidase+3.2.1.20
68382acid phosphatase-3.1.3.2
68382cystine arylamidase-3.4.11.3
68382esterase lipase (C 8)+
68377proline-arylamidase+3.4.11.5
68377beta-galactosidase+3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6
68379catalase-1.11.1.6
68379urease-3.5.1.5
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379pyrazinamidase+3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
48702-+---+-+----+---+++--
5989-+---+++--+-++/---+++-

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119721--++-+-------+-+-+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119721+---------+----------------++-+----++--+---------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
48702-++++----++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
5989human bronchus brush, 57-yr-old womanGöteborgSwedenSWEEurope
48702Human bronchus brush,57-yr-old womanGöteborgSwedenSWEEurope1990-10-03
119721Human, Bronchus brushGöteborgSwedenSWEEurope1990

isolation source categories

Cat1Cat2Cat3
#Host#Human#Female
#Host Body-Site#Oral cavity and airways#Airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_5454.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_124;96_2643;97_3240;98_4073;99_5454&stattab=map
  • Last taxonomy: Actinomyces graevenitzii subclade
  • 16S sequence: AJ540309
  • Sequence Identity:
  • Total samples: 120006
  • soil counts: 1357
  • aquatic counts: 2309
  • animal counts: 115764
  • plant counts: 576

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
59892Risk group (German classification)
1197212Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
23120A.graevenitzii sp. nov. 16S rRNA geneY095891360nuccore55565
5989Actinomyces graevenitzii 16S rRNA gene, strain CCUG 27294TAJ5403091413nuccore55565
124043Actinomyces graevenitzii CCUG 27294 gene for 16S rRNA, partial sequence.LC752337585nuccore55565

External links

@ref: 5989

culture collection no.: DSM 15540, CCUG 27294, CIP 105737, CCM 4739

straininfo link

  • @ref: 69868
  • straininfo: 13434

literature

  • topic: Phylogeny
  • Pubmed-ID: 9226924
  • title: Actinomyces graevenitzii sp. nov., isolated from human clinical specimens.
  • authors: Ramos CP, Falsen E, Alvarez N, Akervall E, Sjoden B, Collins MD
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-47-3-885
  • year: 1997
  • mesh: Actinomyces/*classification/genetics/*isolation & purification, Actinomycosis/*microbiology, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Humans, Molecular Sequence Data, Phylogeny
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5989Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15540)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15540
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23120Cristina Pascual Ramos, Enevold Falsen, Nerea Alvarez, Eva Akervall, Berit Sjödén, Matthew D. Collins10.1099/00207713-47-3-885Actinomyces graevenitzii sp. nov., Isolated from Human Clinical SpecimensIJSEM 47: 885-888 19979226924
39350Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17790
48702Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 27294)https://www.ccug.se/strain?id=27294
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69868Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13434.1StrainInfo: A central database for resolving microbial strain identifiers
119721Curators of the CIPCollection of Institut Pasteur (CIP 105737)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105737
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy