Strain identifier

BacDive ID: 1856

Type strain: Yes

Species: Brevibacterium luteolum

Strain Designation: CF87

Strain history: CIP <- 2003, CCUG <- G. Wauters, UCL, Brussels, Belgium: strain CF87

NCBI tax ID(s): 199591 (species)

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General

@ref: 5673

BacDive-ID: 1856

DSM-Number: 15022

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, flask-shaped, human pathogen

description: Brevibacterium luteolum CF87 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from human peritoneal fluid.

NCBI tax id

  • NCBI tax id: 199591
  • Matching level: species

strain history

@refhistory
5673<- G. Wauters; CF87 (Brevibacterium lutescens) <- B. van Bosterhaut
67770CCUG 46604 <-- G. Wauters CF87 <-- B. Van Bosterhaut.
120691CIP <- 2003, CCUG <- G. Wauters, UCL, Brussels, Belgium: strain CF87

doi: 10.13145/bacdive1856.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Brevibacteriaceae
  • genus: Brevibacterium
  • species: Brevibacterium luteolum
  • full scientific name: Brevibacterium luteolum corrig. Wauters et al. 2003

  • synonyms

    • @ref: 20215
    • synonym: Brevibacterium lutescens

@ref: 5673

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Brevibacteriaceae

genus: Brevibacterium

species: Brevibacterium luteolum

full scientific name: Brevibacterium luteolum Wauters et al. 2003

strain designation: CF87

type strain: yes

Morphology

cell morphology

  • @ref: 120691
  • gram stain: positive
  • cell shape: flask-shaped
  • motility: no

colony morphology

@refincubation periodhemolysis ability
572821 day
1206911

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5673TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
5673COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
32888MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
120691CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
120691CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5673positivegrowth37mesophilic
32888positivegrowth37mesophilic
57282positivegrowth37-42
67770positivegrowth37mesophilic
120691positivegrowth25-41
120691nogrowth10psychrophilic
120691nogrowth15psychrophilic
120691nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57282aerobe
120691obligate aerobe

halophily

  • @ref: 120691
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12069116947citrate-carbon source
1206914853esculin-hydrolysis
120691606565hippurate-hydrolysis
12069117632nitrate-reduction
12069116301nitrite-reduction
12069117632nitrate-respiration

antibiotic resistance

  • @ref: 120691
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120691
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12069115688acetoin-
12069117234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
120691oxidase-
120691beta-galactosidase-3.2.1.23
120691alcohol dehydrogenase-1.1.1.1
120691gelatinase+
120691amylase-
120691DNase+
120691caseinase+3.4.21.50
120691catalase+1.11.1.6
120691tween esterase-
120691gamma-glutamyltransferase-2.3.2.2
120691lysine decarboxylase-4.1.1.18
120691ornithine decarboxylase-4.1.1.17
120691phenylalanine ammonia-lyase-4.3.1.24
120691tryptophan deaminase-
120691urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120691--++-++---++--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
5673human peritoneal fluidBelgiumBELEurope
57282Human peritoneal fluid,patient undergoing dialysisBelgiumBELEurope1999
67770Peritoneal fluid of a patient undergoing dialysis
120691Human, Peritoneal fluidBelgiumBELEurope

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host#Human
#Host Body Product#Fluids
#Host Body-Site#Other#Abdomen

taxonmaps

  • @ref: 69479
  • File name: preview.99_13178.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15817;96_6795;97_8135;98_9977;99_13178&stattab=map
  • Last taxonomy: Brevibacterium luteolum
  • 16S sequence: AJ488509
  • Sequence Identity:
  • Total samples: 24120
  • soil counts: 2899
  • aquatic counts: 3023
  • animal counts: 16149
  • plant counts: 2049

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
5673yes, in single cases1Risk group (German classification)
1206911Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Brevibacterium lutescens partial 16S rRNA gene, type strain CF87T
  • accession: AJ488509
  • length: 1498
  • database: ena
  • NCBI tax ID: 199591

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brevibacterium luteolum DSM 15022GCA_016907415contigncbi199591
66792Brevibacterium luteolum strain CCUG 46604199591.9wgspatric199591
66792Brevibacterium luteolum strain DSM 15022199591.12wgspatric199591
66792Brevibacterium luteolum DSM 150222893558195draftimg199591
67770Brevibacterium luteolum CCUG 46604GCA_013004595contigncbi199591

GC content

  • @ref: 5673
  • GC-content: 68.8

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno91.329no
gram-positiveyes91.124no
anaerobicno98.567yes
aerobicyes90.832yes
halophileyes59.513no
spore-formingno92.764no
glucose-utilyes80.996no
flagellatedno98.401no
thermophileno98.327no
glucose-fermentno94.182no

External links

@ref: 5673

culture collection no.: DSM 15022, CCUG 46604, CIP 108129, JCM 12367, CCM 7149

straininfo link

  • @ref: 71501
  • straininfo: 126463

literature

  • topic: Phylogeny
  • Pubmed-ID: 13130013
  • title: Brevibacterium lutescens sp. nov., from human and environmental samples.
  • authors: Wauters G, Avesani V, Laffineur K, Charlier J, Janssens M, Van Bosterhaut B, Delmee M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02513-0
  • year: 2003
  • mesh: Brevibacterium/classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Environmental Microbiology, Fatty Acids/analysis, Humans, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5673Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15022)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15022
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32888Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5717
57282Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46604)https://www.ccug.se/strain?id=46604
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71501Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID126463.1StrainInfo: A central database for resolving microbial strain identifiers
120691Curators of the CIPCollection of Institut Pasteur (CIP 108129)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108129