Strain identifier

BacDive ID: 1851

Type strain: Yes

Species: Brevibacterium paucivorans

Strain Designation: CF62, CF 62

Strain history: CIP <- 2001, G. Wauters, Brussels, Belgium: strain CF 62

NCBI tax ID(s): 170994 (species)

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General

@ref: 5031

BacDive-ID: 1851

DSM-Number: 13657

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic

description: Brevibacterium paucivorans CF62 is an aerobe, mesophilic bacterium that was isolated from human blood.

NCBI tax id

  • NCBI tax id: 170994
  • Matching level: species

strain history

@refhistory
5031<- G. Wauters; CF62 <- M. Mayer
407052001, G. Wauters, Brussels, Belgium: strain CF 62
67770LMG 19814 <-- G. Wauters CF62 <-- M. Mayer.
121634CIP <- 2001, G. Wauters, Brussels, Belgium: strain CF 62

doi: 10.13145/bacdive1851.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Brevibacteriaceae
  • genus: Brevibacterium
  • species: Brevibacterium paucivorans
  • full scientific name: Brevibacterium paucivorans Wauters et al. 2001

@ref: 5031

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Brevibacteriaceae

genus: Brevibacterium

species: Brevibacterium paucivorans

full scientific name: Brevibacterium paucivorans Wauters et al. 2001

strain designation: CF62, CF 62

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.677
69480100positive
121634nopositiverod-shaped

colony morphology

  • @ref: 121634
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5031TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
40705MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121634CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5031positivegrowth37mesophilic
40705positivegrowth37mesophilic
56879positivegrowth37mesophilic
67770positivegrowth37mesophilic
121634positivegrowth25-41
121634nogrowth10psychrophilic
121634nogrowth15psychrophilic
121634nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
56879aerobe
121634obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.982

halophily

@refsaltgrowthtested relationconcentration
121634NaClpositivegrowth0-4 %
121634NaClnogrowth6 %
121634NaClnogrowth8 %
121634NaClnogrowth10 %

murein

  • @ref: 5031
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
121634citrate-carbon source16947
121634esculin-hydrolysis4853
121634hippurate+hydrolysis606565
121634nitrate-reduction17632
121634nitrite-reduction16301
121634nitrate-respiration17632
68380urea-hydrolysis16199
68380arginine+hydrolysis29016
68380D-mannose-fermentation16024
68380raffinose-fermentation16634
68380L-glutamate-degradation29985
68380nitrate-reduction17632
68380tryptophan-energy source27897

antibiotic resistance

  • @ref: 121634
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
12163435581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6838035581indole-
12163415688acetoin-
12163417234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5
121634oxidase+
121634beta-galactosidase-3.2.1.23
121634alcohol dehydrogenase-1.1.1.1
121634gelatinase-
121634amylase-
121634DNase-
121634caseinase-3.4.21.50
121634catalase+1.11.1.6
121634tween esterase-
121634gamma-glutamyltransferase-2.3.2.2
121634lecithinase-
121634lipase-
121634lysine decarboxylase-4.1.1.18
121634ornithine decarboxylase-4.1.1.17
121634phenylalanine ammonia-lyase-4.3.1.24
121634tryptophan deaminase-
121634urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121634--++-+--+--+---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121634-------------------------------------------------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
56879-+---------------+++-+--+++-+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121634--------------------------------------------------------------+--+--------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
5031human bloodBelgiumBELEurope
56879Human blood
67770Human bloodBelgiumBELEurope
121634Human, BloodBelgiumBELEurope1998

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Fluids#Blood

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
50312Risk group (German classification)
1216341Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5031
  • description: Brevibacterium paucivorans 16S rRNA gene, strain CF 62
  • accession: AJ251463
  • length: 1412
  • database: ena
  • NCBI tax ID: 170994

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brevibacterium paucivorans DSM 13657GCA_016907735contigncbi170994
66792Brevibacterium paucivorans strain DSM 13657170994.6wgspatric170994
66792Brevibacterium paucivorans DSM 136572893675053draftimg170994

GC content

@refGC-contentmethod
503155.8high performance liquid chromatography (HPLC)
6777055.8

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.203no
gram-positiveyes90.398no
anaerobicno98.44no
aerobicyes85.995yes
halophileno53.314no
spore-formingno94.404no
thermophileno96.048yes
glucose-utilyes76.686no
flagellatedno98.433no
glucose-fermentno89.905no

External links

@ref: 5031

culture collection no.: CCUG 45688, DSM 13657, LMG 19814, JCM 11567, ATCC BAA 616, IFM 10521, CIP 107270

straininfo link

  • @ref: 71496
  • straininfo: 14981

literature

  • topic: Phylogeny
  • Pubmed-ID: 11594599
  • title: Brevibacterium paucivorans sp. nov., from human clinical specimens.
  • authors: Wauters G, Charlier J, Janssens M, Delmee M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-5-1703
  • year: 2001
  • mesh: Brevibacterium/chemistry/*classification/genetics/isolation & purification, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis/genetics, Genes, rRNA, Gram-Positive Bacterial Infections/*microbiology, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitle
5031Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13657)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13657
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40705Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4687
56879Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45688)https://www.ccug.se/strain?id=45688
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71496Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14981.1StrainInfo: A central database for resolving microbial strain identifiers
121634Curators of the CIPCollection of Institut Pasteur (CIP 107270)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107270