Strain identifier

BacDive ID: 1848

Type strain: Yes

Species: Brevibacterium mcbrellneri

Strain Designation: E2cr, 669

Strain history: CIP <- 1995, M.E. McBride, Baylor Coll. Med., Houston, TX, USA: strain E2cr

NCBI tax ID(s): 585530 (strain), 53363 (species)

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General

@ref: 3646

BacDive-ID: 1848

DSM-Number: 9583

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic

description: Brevibacterium mcbrellneri E2cr is an aerobe, mesophilic bacterium that was isolated from infected genital hair of patient with white piedra.

NCBI tax id

NCBI tax idMatching level
585530strain
53363species

strain history

@refhistory
3646<- G. Funke, 669 <- M.E. McBride, E2cr
411241995, M.E. McBride, Baylor Coll. Med., Houston, TX, USA: strain E2cr
67770CCUG 37313 <-- G. Funke DMMZ 669 <-- M. E. McBride E2cr.
120864CIP <- 1995, M.E. McBride, Baylor Coll. Med., Houston, TX, USA: strain E2cr

doi: 10.13145/bacdive1848.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Brevibacteriaceae
  • genus: Brevibacterium
  • species: Brevibacterium mcbrellneri
  • full scientific name: Brevibacterium mcbrellneri McBride et al. 1994

@ref: 3646

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Brevibacteriaceae

genus: Brevibacterium

species: Brevibacterium mcbrellneri

full scientific name: Brevibacterium mcbrellneri McBride et al. 1994 emend. Nouioui et al. 2018

strain designation: E2cr, 669

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.797
69480100positive
120864nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3646TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
41124MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
3646COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
120864CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
120864CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
3646positivegrowth37mesophilic
41124positivegrowth30mesophilic
53600positivegrowth37mesophilic
67770positivegrowth37mesophilic
120864positivegrowth30-37mesophilic
120864nogrowth5psychrophilic
120864nogrowth10psychrophilic
120864nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
53600aerobe
120864obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.996

murein

  • @ref: 3646
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
120864citrate-carbon source16947
120864nitrate+reduction17632
120864nitrite-reduction16301
120864nitrate-respiration17632
68379nitrate-reduction17632
68379esculin-hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-glucose-fermentation17634
68379D-ribose-fermentation16988
68379D-xylose-fermentation65327
68379D-mannitol-fermentation16899
68379maltose-fermentation17306
68379lactose-fermentation17716
68379sucrose-fermentation17992
68379glycogen-fermentation28087

antibiotic resistance

  • @ref: 120864
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120864
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
120864oxidase-
120864beta-galactosidase+3.2.1.23
120864alcohol dehydrogenase-1.1.1.1
120864gelatinase+
120864catalase+1.11.1.6
120864tween esterase-
120864gamma-glutamyltransferase+2.3.2.2
120864lecithinase-
120864lipase+
120864lysine decarboxylase-4.1.1.18
120864ornithine decarboxylase-4.1.1.17
120864phenylalanine ammonia-lyase-4.3.1.24
120864tryptophan deaminase+
120864urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    53600C16:0216
    53600C13:0 ANTEISO112.701
    53600C14:0 ISO113.618
    53600C15:0 ANTEISO40.814.711
    53600C15:0 ISO12.114.621
    53600C16:0 iso14.215.626
    53600C17:0 anteiso23.616.722
    53600C17:0 iso1.816.629
    53600C18:1 ω9c1.717.769
    53600C18:2 ω6,9c/C18:0 ANTE1.917.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
3646--------------------+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120864-+++------+----+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120864+---+----++++--------+/------++-+--+/------+--------+/-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationisolation date
3646infected genital hair of patient with white piedraUSAUSANorth America
53600Human genital hair nodules,patient w/white piedra
67770Genital hair nodule of a patient with white piedra
120864Human, Genital hairUnited States of AmericaUSANorth AmericaHouston, Texas1985

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Host Body-Site#Urogenital tract
#Host Body-Site#Organ#Skin, Nail, Hair

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
36462Risk group (German classification)
1208641Risk group (French classification)

Sequence information

16S sequences

  • @ref: 3646
  • description: B.mcbrellneri 16S ribosomal RNA
  • accession: X93594
  • length: 1404
  • database: ena
  • NCBI tax ID: 585530

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brevibacterium mcbrellneri ATCC 49030585530.3wgspatric585530
66792Brevibacterium mcbrellneri ATCC 49030647000216draftimg585530
67770Brevibacterium mcbrellneri ATCC 49030GCA_000178455contigncbi585530

GC content

@refGC-contentmethod
364663thermal denaturation, midpoint method (Tm)
6777063.05thermal denaturation, midpoint method (Tm)
6777058genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveyes90.754no
anaerobicno98.892no
halophileyes50.517no
spore-formingno94.14no
glucose-utilyes79.913no
thermophileno97.088no
flagellatedno98.191no
aerobicyes91.765yes
motileno94.178no
glucose-fermentno89.311yes

External links

@ref: 3646

culture collection no.: DSM 9583, ATCC 49030, CCUG 37313, JCM 11682, CIP 104342

straininfo link

  • @ref: 71493
  • straininfo: 42257

literature

  • topic: Phylogeny
  • Pubmed-ID: 8411085
  • title: A new Brevibacterium sp. isolated from infected genital hair of patients with white piedra.
  • authors: McBride ME, Ellner KM, Black HS, Clarridge JE, Wolf JE
  • journal: J Med Microbiol
  • DOI: 10.1099/00222615-39-4-255
  • year: 1993
  • mesh: Anti-Bacterial Agents/pharmacology, Base Composition, Brevibacterium/classification/cytology/*isolation & purification/physiology, Cell Wall/chemistry, DNA, Bacterial/chemistry, Fatty Acids/analysis, Genitalia, Hair/*microbiology, Humans, Oxidation-Reduction, Piedra/*microbiology, Ribose/metabolism, Tellurium/metabolism, Terminology as Topic, *Trichosporon
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3646Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9583)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9583
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41124Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16240
53600Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37313)https://www.ccug.se/strain?id=37313
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71493Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42257.1StrainInfo: A central database for resolving microbial strain identifiers
120864Curators of the CIPCollection of Institut Pasteur (CIP 104342)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104342