Strain identifier

BacDive ID: 1847

Type strain: Yes

Species: Bacillus halotolerans

Strain Designation: WS 1087, 6721

Strain history: CIP <- 1967, B. Delaporte, Inst. Pasteur, Paris, France

NCBI tax ID(s): 260554 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 3408

BacDive-ID: 1847

DSM-Number: 8802

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, motile

description: Bacillus halotolerans WS 1087 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 260554
  • Matching level: species

strain history

@refhistory
422121967, B. Delaporte, Inst. Pasteur, Paris, France
3408<- H. Seiler, WS 1087 <- ATCC <- B. Delaporte, 6721 <- A. Sasson
67770CIP 67.21 <-- B. Delaporte 6721.
119823CIP <- 1967, B. Delaporte, Inst. Pasteur, Paris, France

doi: 10.13145/bacdive1847.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus halotolerans
  • full scientific name: Bacillus halotolerans (Delaporte and Sasson 1967) Tindall 2017

  • synonyms

    @refsynonym
    20215Bacillus malacitensis
    20215Bacillus halotolerans
    20215Bacillus axarquiensis
    20215Brevibacterium halotolerans

@ref: 3408

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Actinomycetales

family: Bacillaceae

genus: Bacillus

species: Bacillus halotolerans

full scientific name: Bacillus halotolerans (Delaporte and Sasson 1967) Tindall 2017

strain designation: WS 1087, 6721

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes96.242
69480100positive
119823yespositiverod-shaped

colony morphology

@refcolony colorincubation periodmedium used
20210Ivory (1014)10-14 daysISP 2
20210Light ivory (1015)10-14 daysISP 3
20210Rapeseed yellow (1021)10-14 daysISP 4
20210Traffic yellow (1023)10-14 daysISP 6

multicellular morphology

@refforms multicellular complexmedium name
20210noISP 2
20210noISP 3
20210noISP 4
20210noISP 6

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
20210ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20210ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20210ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20210ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
42212MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
3408TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yesName: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/92
119823CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
119823CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
20210positiveoptimum28mesophilic
42212positivegrowth30mesophilic
57815positivegrowth30mesophilic
3408positivegrowth30mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57815aerobe
119823obligate aerobe

spore formation

@refspore formationconfidence
69481yes94
69480yes100

halophily

  • @ref: 20210
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

murein

  • @ref: 3408
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2021017234glucose-
2021022599arabinose-
2021017992sucrose-
2021018222xylose-
2021017268myo-inositol-
2021029864mannitol-
2021028757fructose-
2021026546rhamnose-
2021016634raffinose-
2021062968cellulose-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
11982317632nitrate+reduction
11982316301nitrite-reduction
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol+fermentation
6837917306maltose+fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
11982335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
119823oxidase-
119823catalase+1.11.1.6
119823urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
20210--+------++---++---

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20210--+------++---++---
119823++-------++-+-++----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
20210+--------++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3408soilMoroccoMARAfrica
57815SoilMoroccoMARAfrica
67770Arid soilMoroccoMARAfrica
119823Environment, SoilMoroccoMARAfrica

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_21.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_17;97_17;98_20;99_21&stattab=map
  • Last taxonomy: Bacillus
  • 16S sequence: AM747812
  • Sequence Identity:
  • Total samples: 18201
  • soil counts: 3768
  • aquatic counts: 2854
  • animal counts: 9674
  • plant counts: 1905

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
202101
34081Risk group (German classification)
1198231Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218[Brevibacterium] halotolerans 16S rRNA gene, strain DSM 8802AM7478121545ena260554
67770Brevibacterium halotolerans partial 16S rRNA gene, type strain LMG 21660TAJ6203681513ena260554

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792[Brevibacterium] halotolerans strain ATCC 25096260554.3wgspatric260554
66792Bacillus halotolerans ATCC 250962716884207draftimg260554
67770Bacillus halotolerans ATCC 25096GCA_001517105contigncbi260554

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes94no
motileyes90.157no
gram-positiveyes92.79no
anaerobicno99.252no
aerobicyes90.169yes
halophileyes87.582no
spore-formingyes96.686no
glucose-utilyes88.999no
flagellatedyes89.378no
thermophileno97.62yes
glucose-fermentno91.545yes

External links

@ref: 3408

culture collection no.: CCUG 47676, CIP 67.21, DSM 8802, ATCC 25096, JCM 12400, BCRC 14861

straininfo link

  • @ref: 71492
  • straininfo: 389308

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27717990Reclassification of Brevibacterium halotolerans DSM8802 as Bacillus halotolerans comb. nov. Based on Microbial and Biochemical Characterization and Multiple Gene Sequence.Ben-Gad D, Gerchman YCurr Microbiol10.1007/s00284-016-1143-42016Bacillus/*classification/genetics/growth & development/isolation & purification, Bacterial Proteins/genetics, Bacterial Typing Techniques, Brevibacterium/*classification/genetics/growth & development/isolation & purification, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Environmental Microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Spores, Bacterial/classification/genetics/growth & development/isolation & purificationEnzymology
Metabolism32747997Polyhydroxyalkanoate synthesis by bacteria isolated from landfill and ETP with pomegranate peels as carbon source.Rayasam V, Chavan P, Kumar TArch Microbiol10.1007/s00203-020-01995-92020Bacillus/isolation & purification/*metabolism, Carbon, Cupriavidus necator/*metabolism, Polyhydroxyalkanoates/*biosynthesis, Pomegranate/*microbiology, *Waste Disposal FacilitiesEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3408Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8802)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8802
20210Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM8802.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42212Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10302
57815Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47676)https://www.ccug.se/strain?id=47676
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71492Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389308.1StrainInfo: A central database for resolving microbial strain identifiers
119823Curators of the CIPCollection of Institut Pasteur (CIP 67.21)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2067.21