Strain identifier

BacDive ID: 1842

Type strain: Yes

Species: Brevibacterium linens

Strain Designation: 56b, 56-B

Strain history: CIP <- 1984, ATCC <- R.S. Breed: strain 56-B <- C. Kelly <- H. Weigmann

NCBI tax ID(s): 1255617 (strain), 1703 (species)

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General

@ref: 8804

BacDive-ID: 1842

DSM-Number: 20425

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped

description: Brevibacterium linens 56b is an aerobe, mesophilic, Gram-positive bacterium that was isolated from harzer cheese.

NCBI tax id

NCBI tax idMatching level
1703species
1255617strain

strain history

@refhistory
8804<- H. Seiler <- ATCC <- R.S. Breed, 56b <- C. Kelly <- H. Weigmann
67770ATCC 9172 <-- R. S. Breed 56b <-- C. Kelly <-- H. Weigmann.
120614CIP <- 1984, ATCC <- R.S. Breed: strain 56-B <- C. Kelly <- H. Weigmann

doi: 10.13145/bacdive1842.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Brevibacteriaceae
  • genus: Brevibacterium
  • species: Brevibacterium linens
  • full scientific name: Brevibacterium linens (Wolff 1910) Breed 1953 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium linens

@ref: 8804

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Brevibacteriaceae

genus: Brevibacterium

species: Brevibacterium linens

full scientific name: Brevibacterium linens (Wolff 1910) Breed 1953

strain designation: 56b, 56-B

type strain: yes

Morphology

cell morphology

  • @ref: 120614
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 120614

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8804CORYNEBACTERIUM AGAR (DSMZ Medium 53)yeshttps://mediadive.dsmz.de/medium/53Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
35240MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
8804TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
120614CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8804positivegrowth30mesophilic
35240positivegrowth30mesophilic
57816positivegrowth30mesophilic
67770positivegrowth30mesophilic
120614positivegrowth15-30
120614nogrowth10psychrophilic
120614nogrowth37mesophilic
120614nogrowth41thermophilic
120614nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57816aerobe
120614obligate aerobe

halophily

  • @ref: 120614
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

murein

  • @ref: 8804
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12061416947citrate+carbon source
1206144853esculin-hydrolysis
120614606565hippurate+hydrolysis
12061417632nitrate+reduction
12061416301nitrite-reduction
12061417632nitrate-respiration

antibiotic resistance

  • @ref: 120614
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120614
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12061415688acetoin-
12061417234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120614oxidase-
120614beta-galactosidase-3.2.1.23
120614alcohol dehydrogenase-1.1.1.1
120614gelatinase-
120614amylase-
120614DNase+
120614caseinase+3.4.21.50
120614catalase+1.11.1.6
120614tween esterase-
120614gamma-glutamyltransferase-2.3.2.2
120614lecithinase-
120614lipase-
120614lysine decarboxylase-4.1.1.18
120614ornithine decarboxylase-4.1.1.17
120614phenylalanine ammonia-lyase-4.3.1.24
120614protease+
120614tryptophan deaminase-
120614urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120614-+++-+-----+--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120614--+----------------------------+----------------------+-+-----++--------+----------------------+-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
8804harzer cheese
57816Cheese,HarzerkäseKielGermanyDEUEurope
67770Dairy productKielGermanyDEUEurope
120614Food, Cheese, HarzerkaseKielGermanyDEUEurope

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_91932.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15817;96_81;97_619;98_63319;99_91932&stattab=map
  • Last taxonomy: Brevibacterium
  • 16S sequence: X77451
  • Sequence Identity:
  • Total samples: 42
  • soil counts: 2
  • aquatic counts: 5
  • animal counts: 33
  • plant counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
88041Risk group (German classification)
1206141Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Brevibacterium linens strain ATCC 9172 16S-23S ribosomal intergenic spacer sequenceU59267441ena1703
20218Brevibacterium linens DSM20425T 16S ribosomal RNA gene, partial sequenceAF426135631ena1703
8804B.linens (DSM 20425) 16S rRNA geneX774511473ena1703

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brevibacterium linens ATCC 91721255617.4wgspatric1255617
66792Brevibacterium linens ATCC 91721255617.3wgspatric1255617
66792Brevibacterium linens ATCC 91722600255378draftimg1255617
67770Brevibacterium linens ATCC 9172GCA_900169165contigncbi1255617
67770Brevibacterium linens ATCC 9172GCA_008868375contigncbi1255617

GC content

@refGC-contentmethod
880463.4
6777063.4thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno93.423no
gram-positiveyes90.946no
anaerobicno99.532no
aerobicyes95.32no
halophileyes63.513no
spore-formingno88.727no
thermophileno99.329no
glucose-utilyes87.053no
flagellatedno97.204no
glucose-fermentno93.3no

External links

@ref: 8804

culture collection no.: DSM 20425, ATCC 9172, NCIB 9909, CCUG 47677, CIP 101125, IMET 11075, JCM 1327, NCIMB 9909, BCRC 10631, CECT 69, CGMCC 1.1890, HAMBI 2038, IAM 12437, IFO 12142, IMSNU 12001, KCTC 9099, LMG 21451, MTCC 268, NBRC 12142, NRRL B-4210, VKM Ac-2112, VTT E-94529

straininfo link

  • @ref: 71487
  • straininfo: 8181

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity11849329Characterization of an antibacterial peptide produced by Brevibacterium linens.Motta AS, Brandelli AJ Appl Microbiol10.1046/j.1365-2672.2002.01490.x2002Anti-Bacterial Agents/*metabolism/*pharmacology, Bacteria/*drug effects, Bacteriocins/*biosynthesis/*pharmacology, Brevibacterium/drug effects/growth & development/*metabolism, Cheese/microbiology, Corynebacterium/drug effects, Culture Media, Endopeptidases/metabolism, Food Preservation/methods, Hot Temperature, Hydrogen-Ion Concentration, Listeria monocytogenes/drug effects, Microbial Sensitivity Tests, *PeptidesMetabolism
Phylogeny12882170High cell density cultivation of Brevibacterium linens and formation of proteinases and lipase.Adamitsch BF, Karner F, Hampel WABiotechnol Lett10.1023/a:10234025298822003Ammonium Sulfate/pharmacology, Brevibacterium/classification/drug effects/*enzymology/*growth & development, Cell Count, Cell Culture Techniques/*methods, Cells, Cultured, Culture Media/pharmacology, Endopeptidases/biosynthesis, Enzyme Activation, Lactic Acid/pharmacology, Leucyl Aminopeptidase/biosynthesis, Lipase/*biosynthesis, Peptones/pharmacology, Quality Control, Soybean Proteins/pharmacology, Species SpecificityCultivation
Phylogeny27654846Brevibacterium sediminis sp. nov., isolated from deep-sea sediments from the Carlsberg and Southwest Indian Ridges.Chen P, Zhang L, Wang J, Ruan J, Han X, Huang YInt J Syst Evol Microbiol10.1099/ijsem.0.0015062016Bacterial Typing Techniques, Base Composition, Brevibacterium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/microbiology, Indian Ocean, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8804Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20425)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20425
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35240Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/12666
57816Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47677)https://www.ccug.se/strain?id=47677
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71487Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8181.1StrainInfo: A central database for resolving microbial strain identifiers
120614Curators of the CIPCollection of Institut Pasteur (CIP 101125)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20101125