General

@ref: 5462

BacDive-ID: 18147

DSM-Number: 14648

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanocalculus taiwanensis P2F9705 is an anaerobe, mesophilic archaeon that was isolated from water sample.

NCBI tax id

• NCBI tax id: 106207
• Matching level: species

strain history

• @ref: 5462
• history: <- M.-C. Lai, National Chung Hsing Univ., Taiwan; P2F9705 <- M.-C. Lai {1996}

doi: 10.13145/bacdive18147.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanomicrobiales
• family: Methanocalculaceae
• genus: Methanocalculus
• species: Methanocalculus taiwanensis
• full scientific name: Methanocalculus taiwanensis Lai et al. 2002

@ref: 5462

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanocalculaceae

genus: Methanocalculus

species: Methanocalculus taiwanensis

full scientific name: Methanocalculus taiwanensis Lai et al. 2002

strain designation: P2F9705

type strain: no

Culture and growth conditions

culture medium

• @ref: 5462
• name: METHANOCALCULUS MB MEDIUM (DSMZ Medium 924)
• growth: yes
• link: https://mediadive.dsmz.de/medium/924
• composition: Name: METHANOCALCULUS MB MEDIUM (DSMZ Medium 924) Composition: Na-formate 6.72601 g/l NaHCO3 3.95648 g/l Trypticase peptone 1.97824 g/l Yeast extract 1.97824 g/l Na-acetate 1.58259 g/l MgCl2 x 6 H2O 0.98912 g/l NH4Cl 0.98912 g/l KCl 0.49456 g/l K2HPO4 0.395648 g/l Na2S x 9 H2O 0.24728 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

• @ref: 5462
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 5462
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 5462
• sample type: water sample
• geographic location: Taiwan, Eriln Shi estuary environment
• country: China
• origin.country: CHN
• continent: Asia

isolation source categories

• Cat1: #Environmental
• Cat2: #Aquatic

Safety information

risk assessment

• @ref: 5462
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 5462
• description: Methanocalculus taiwanensis strain P2F9705 16S ribosomal RNA gene, partial sequence
• accession: AF411470
• length: 1260
• database: ena
• NCBI tax ID: 106207

External links

@ref: 5462

culture collection no.: DSM 14648, OCM 672

literature

• topic: Phylogeny
• Pubmed-ID: 12361289
• title: Methanocalculus taiwanensis sp. nov., isolated from an estuarine environment.
• authors: Lai MC, Chen SC, Shu CM, Chiou MS, Wang CC, Chuang MJ, Hong TY, Liu CC, Lai LJ, Hua JJ
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/00207713-52-5-1799
• year: 2002
• mesh: Archaeal Proteins/analysis, Base Sequence, DNA, Archaeal/genetics, DNA, Ribosomal/genetics, Drug Resistance, Multiple, Genes, Archaeal, Methanomicrobiales/*classification/genetics/*isolation & purification/metabolism, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Archaeal/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Taiwan, Terminology as Topic, Water Microbiology
• topic2: Genetics

Reference