Strain identifier

BacDive ID: 18145

Type strain: No

Species: Methanocalculus chunghsingensis

Strain Designation: K1F9705c

Strain history: <- M.-C. Lai, National Chung Hsing Univ., Taiwan; K1F9705c <- M.-C. Lai {1997}

NCBI tax ID(s): 156457 (species)

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General

@ref: 5431

BacDive-ID: 18145

DSM-Number: 14646

keywords: 16S sequence, Archaea, anaerobe, mesophilic

description: Methanocalculus chunghsingensis K1F9705c is an anaerobe, mesophilic archaeon that was isolated from sediment sample of a marine water aquaculture fishpond.

NCBI tax id

  • NCBI tax id: 156457
  • Matching level: species

strain history

  • @ref: 5431
  • history: <- M.-C. Lai, National Chung Hsing Univ., Taiwan; K1F9705c <- M.-C. Lai {1997}

doi: 10.13145/bacdive18145.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanomicrobiales
  • family: Methanocalculaceae
  • genus: Methanocalculus
  • species: Methanocalculus chunghsingensis
  • full scientific name: Methanocalculus chunghsingensis Lai et al. 2004

@ref: 5431

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanomicrobiales

family: Methanocalculaceae

genus: Methanocalculus

species: Methanocalculus chunghsingensis

full scientific name: Methanocalculus chunghsingensis Lai et al. 2004

strain designation: K1F9705c

type strain: no

Culture and growth conditions

culture medium

  • @ref: 5431
  • name: METHANOCALCULUS MB MEDIUM (DSMZ Medium 924)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/924
  • composition: Name: METHANOCALCULUS MB MEDIUM (DSMZ Medium 924) Composition: Na-formate 6.72601 g/l NaHCO3 3.95648 g/l Trypticase peptone 1.97824 g/l Yeast extract 1.97824 g/l Na-acetate 1.58259 g/l MgCl2 x 6 H2O 0.98912 g/l NH4Cl 0.98912 g/l KCl 0.49456 g/l K2HPO4 0.395648 g/l Na2S x 9 H2O 0.24728 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

  • @ref: 5431
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 5431
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 5431
  • sample type: sediment sample of a marine water aquaculture fishpond
  • geographic location: Taiwan, Wong-kong
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_193368.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17845;96_69215;97_89913;98_122246;99_193368&stattab=map
  • Last taxonomy: Methanocalculus chunghsingensis subclade
  • 16S sequence: AF321115
  • Sequence Identity:
  • Total samples: 1
  • aquatic counts: 1

Safety information

risk assessment

  • @ref: 5431
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5431
  • description: Methanocalculus sp. K1F9705c 16S ribosomal RNA, partial sequence
  • accession: AF321115
  • length: 1251
  • database: ena
  • NCBI tax ID: 156457

External links

@ref: 5431

culture collection no.: DSM 14646

straininfo link

  • @ref: 87131
  • straininfo: 143377

literature

  • topic: Phylogeny
  • Pubmed-ID: 14742478
  • title: Methanocalculus chunghsingensis sp. nov., isolated from an estuary and a marine fishpond in Taiwan.
  • authors: Lai MC, Lin CC, Yu PH, Huang YF, Chen SC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02761-0
  • year: 2004
  • mesh: Base Sequence, DNA Primers, DNA, Ribosomal/genetics, Fisheries, Formates/metabolism, Methanomicrobiales/*classification/genetics/isolation & purification, Microbial Sensitivity Tests, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Taiwan
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
5431Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14646)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14646
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87131Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID143377.1StrainInfo: A central database for resolving microbial strain identifiers