Strain identifier

BacDive ID: 1814

Type strain: Yes

Species: Bradyrhizobium liaoningense

Strain Designation: 2281

Strain history: 1996, L.M. Xu, Soils and Fertitlizers Inst., Beijing, China: strain ESG 2281

NCBI tax ID(s): 43992 (species)

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General

@ref: 17488

BacDive-ID: 1814

DSM-Number: 24092

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Bradyrhizobium liaoningense 2281 is a mesophilic, Gram-negative, rod-shaped bacterium that forms circular colonies.

NCBI tax id

  • NCBI tax id: 43992
  • Matching level: species

strain history

@refhistory
17488<- CECT <- E. Velázquez <- USDA, Beltsville
413461996, L.M. Xu, Soils and Fertitlizers Inst., Beijing, China: strain ESG 2281

doi: 10.13145/bacdive1814.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Nitrobacteraceae
  • genus: Bradyrhizobium
  • species: Bradyrhizobium liaoningense
  • full scientific name: Bradyrhizobium liaoningense Xu et al. 1995

@ref: 17488

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Nitrobacteraceae

genus: Bradyrhizobium

species: Bradyrhizobium liaoningense

full scientific name: Bradyrhizobium liaoningense Xu et al. 1995

strain designation: 2281

type strain: yes

Morphology

cell morphology

  • @ref: 23329
  • gram stain: negative
  • cell length: 1.5-4.5 µm
  • cell width: 0.5 µm
  • cell shape: rod-shaped

colony morphology

  • @ref: 23329
  • colony size: 0.2-1 mm
  • colony shape: circular
  • incubation period: 7-14 days
  • medium used: YMA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17488RHIZOBIUM MEDIUM (DSMZ Medium 98)yeshttps://mediadive.dsmz.de/medium/98Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
23329YMAyes
41346MEDIUM 161 - for Bradyrhizobium liaoningenseyesDistilled water make up to (1000.000 ml);Sodium chloride (0.100 g);Magnesium sulphate heptahydrate (0.200 g);Agar (15.000 g);Yeast extract (0.600 g);Potassium di-hydrogen phosphate (0.600 g);Sodium glutamate (6.000 g);Calcium carbonate (3.000 g);Arabinose

culture temp

@refgrowthtypetemperaturerange
17488positivegrowth28mesophilic
23329nogrowth35mesophilic
23329nogrowth37mesophilic
23329positiveoptimum25-30mesophilic
41346positivegrowth30mesophilic

culture pH

@refabilitytypepH
23329positiveoptimum6.5-6.8
23329nogrowth10

Physiology and metabolism

spore formation

  • @ref: 23329
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
23329NaClnoresistant0.1 M
23329NaClnoresistant0.2 M
23329NaClnoresistant0.3 M

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2332917057cellobiose-carbon source
233296731melezitose-carbon source
2332928053melibiose-carbon source
2332916551D-trehalose-carbon source
2332917113erythritol-carbon source
233294853esculin-carbon source
2332930742ethylene glycol-carbon source
2332917754glycerol-carbon source
2332917268myo-inositol-carbon source
2332915443inulin-carbon source
2332917716lactose-carbon source
2332917306maltose-carbon source
2332916634raffinose-carbon source
2332917814salicin-carbon source
2332917992sucrose-carbon source
23329132950tartrate-carbon source
2332917821thymine-nitrogen source
2332922599arabinose+carbon source
2332929987glutamate+carbon source
2332915963ribitol+/-carbon source
2332916947citrate+/-carbon source
2332918333D-arabitol+/-carbon source
2332932528turanose+/-carbon source
2332928757fructose+/-carbon source
2332928260galactose+/-carbon source
2332917234glucose+/-carbon source
2332915792malonate+/-carbon source
2332929864mannitol+/-carbon source
2332930911sorbitol+/-carbon source
2332933954tagatose+/-carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is intermediateintermediate conc.is sensitivesensitivity conc.
2332950845doxycyclineyesyes25 Unit
2332927644chlortetracyclinyesyes25 µg
2332927701oxytetracyclineyesyes25 µg
2332922907bleomycinyesyes5 µg
2332917698chloramphenicolyesyes50 µg
2332927902tetracyclineyesyes50 Unit
2332917334penicillinyesyes25 µg
2332917334penicillinyesyes50 µg

Isolation, sampling and environmental information

isolation

  • @ref: 41346
  • country: China
  • origin.country: CHN
  • continent: Asia

taxonmaps

  • @ref: 69479
  • File name: preview.99_445.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_297;97_329;98_370;99_445&stattab=map
  • Last taxonomy: Bradyrhizobium
  • 16S sequence: AB029402
  • Sequence Identity:
  • Total samples: 12887
  • soil counts: 5001
  • aquatic counts: 2827
  • animal counts: 4320
  • plant counts: 739

Safety information

risk assessment

  • @ref: 17488
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bradyrhizobium liaoningense gene for 16S ribosomal RNA, partial sequenceAB0294021425ena43992
20218Bradyrhizobium liaoningense strain 2281 16S ribosomal RNA gene, partial sequenceAF3631321336ena43992
20218Bradyrhizobium liaoningense strain LMG 18230 16S ribosomal RNA gene, partial sequence; internal transcribed spacer, complete sequence; and 23S ribosomal RNA, partial sequenceAF345256824ena43992
20218Bradyrhizobium liaoningense 16S rRNA gene, strain LMG 18230AJ2508131430ena43992
20218Bradyrhizobium liaoningense gene for 16S ribosomal RNA, partial sequence, strain: NBRC 100396AB5100001414ena43992
20218Bradyrhizobium liaoningense genes for 16S-23S rRNA internal transcribed spacer, tRNA-Ile, tRNA-Ala, partial and sequence, strain: NBRC 100396AB510001800ena43992
20218Bradyrhizobium liaoningense DNA, 16S-23S ITS1 region, tRNA-Ile, tRNA-Ala, strain: USDA 3622AB100759776ena43992
20218Bradyrhizobium liaoningense strain 2281 (USDA 3622) 16S ribosomal RNA gene, partial sequence; tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF2085132709ena43992

External links

@ref: 17488

culture collection no.: DSM 24092, CECT 4845, USDA 3622

straininfo link

  • @ref: 71460
  • straininfo: 11484

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22544787Bradyrhizobium daqingense sp. nov., isolated from soybean nodules.Wang JY, Wang R, Zhang YM, Liu HC, Chen WF, Wang ET, Sui XH, Chen WXInt J Syst Evol Microbiol10.1099/ijs.0.034280-02012Bacterial Typing Techniques, Base Composition, Bradyrhizobium/*classification/genetics/isolation & purification, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Soybeans/*microbiology, Ubiquinone/analysisGenetics
Phylogeny28214058Phylogenetic multilocus sequence analysis of indigenous slow-growing rhizobia nodulating cowpea (Vigna unguiculata L.) in Greece.Tampakaki AP, Fotiadis CT, Ntatsi G, Savvas DSyst Appl Microbiol10.1016/j.syapm.2017.01.0012017DNA, Ribosomal Spacer/genetics, Genes, Essential, Greece, *Multilocus Sequence Typing, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizobium/*classification/*genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Symbiosis/genetics, Vigna/*microbiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17488Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24092)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24092
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23329L. M. XU, C. GE, Z. CUI, J. LI, H. FAN10.1099/00207713-45-4-706Bradyrhizobium liaoningense sp. nov., Isolated from the Root Nodules of SoybeansIJSEM 45: 706-711 19957547289
41346Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16813
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71460Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID11484.1StrainInfo: A central database for resolving microbial strain identifiers