Strain identifier
BacDive ID: 1812
Type strain: 
Species: Bradyrhizobium canariense
Strain Designation: BTA-1
Strain history: <- P. Vinuesa <- M. León-Barrios; BTA-1
NCBI tax ID(s): 255045 (species)
version 8.1 (current version)
General
@ref: 6530
BacDive-ID: 1812
DSM-Number: 16623
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile
description: Bradyrhizobium canariense BTA-1 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodules of Chamaecytisus proliferus subsp. proliferus var. palmensis .
NCBI tax id
- NCBI tax id: 255045
- Matching level: species
strain history
- @ref: 6530
- history: <- P. Vinuesa <- M. León-Barrios; BTA-1
doi: 10.13145/bacdive1812.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Nitrobacteraceae
- genus: Bradyrhizobium
- species: Bradyrhizobium canariense
- full scientific name: Bradyrhizobium canariense Vinuesa et al. 2005
@ref: 6530
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Nitrobacteraceae
genus: Bradyrhizobium
species: Bradyrhizobium canariense
full scientific name: Bradyrhizobium canariense Vinuesa et al. 2005
strain designation: BTA-1
type strain: yes
Morphology
cell morphology
- @ref: 23327
- gram stain: negative
- motility: yes
- flagellum arrangement: monotrichous, polar
colony morphology
- @ref: 23327
- colony size: 1-1.5 mm
- colony color: white, creamy
- incubation period: 7 days
- medium used: YMA (pH 6.8)
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 6530 | RHIZOBIUM MEDIUM (DSMZ Medium 98) | yes | https://mediadive.dsmz.de/medium/98 | Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water |
| 23326 | YMA (pH 6.8) | yes |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6530 | positive | growth | 30 | mesophilic |
| 23327 | no | growth | 37 | mesophilic |
| 23327 | positive | optimum | 28-30 | mesophilic |
culture pH
- @ref: 23327
- ability: positive
- type: growth
- pH: 9
- PH range: alkaliphile
Physiology and metabolism
oxygen tolerance
- @ref: 23327
- oxygen tolerance: aerobe
spore formation
- @ref: 23327
- spore formation: no
halophily
- @ref: 23327
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 1 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 23327 | 18135 | catechol | - | carbon source |
| 23327 | 16551 | D-trehalose | - | carbon source |
| 23327 | 15443 | inulin | - | carbon source |
| 23327 | 17266 | L-sorbose | - | carbon source |
| 23327 | 17716 | lactose | - | carbon source |
| 23327 | 28053 | melibiose | - | carbon source |
| 23327 | 28017 | starch | - | carbon source |
| 23327 | 17992 | sucrose | - | carbon source |
| 23327 | 15428 | glycine | - | nitrogen source |
| 23327 | 16947 | citrate | + | carbon source |
| 23327 | 17108 | D-arabinose | + | carbon source |
| 23327 | 15824 | D-fructose | + | carbon source |
| 23327 | 12936 | D-galactose | + | carbon source |
| 23327 | 17634 | D-glucose | + | carbon source |
| 23327 | 16024 | D-mannose | + | carbon source |
| 23327 | 16988 | D-ribose | + | carbon source |
| 23327 | 65327 | D-xylose | + | carbon source |
| 23327 | 29806 | fumarate | + | carbon source |
| 23327 | 17754 | glycerol | + | carbon source |
| 23327 | 62345 | L-rhamnose | + | carbon source |
| 23327 | 29864 | mannitol | + | carbon source |
| 23327 | 30911 | sorbitol | + | carbon source |
| 23327 | 30031 | succinate | + | carbon source |
| 23327 | 18050 | L-glutamine | + | nitrogen source |
Isolation, sampling and environmental information
isolation
- @ref: 6530
- sample type: root nodules of Chamaecytisus proliferus subsp. proliferus var. palmensis (Papilionoideae: Genisteae)
- host species: Chamaecytisus proliferus subsp. proliferus
- geographic location: Canary Islands, Tenerife, La Laguna
- country: Spain
- origin.country: ESP
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Plants | #Shrub (Scrub) |
| #Host Body-Site | #Plant | #Root nodule |
taxonmaps
- @ref: 69479
- File name: preview.99_445.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_297;97_329;98_370;99_445&stattab=map
- Last taxonomy: Bradyrhizobium
- 16S sequence: AJ558025
- Sequence Identity:
- Total samples: 12887
- soil counts: 5001
- aquatic counts: 2827
- animal counts: 4320
- plant counts: 739
Safety information
risk assessment
- @ref: 6530
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 23327 | Bradyrhizobium canariense 16S ribosomal RNA gene, partial sequence | AY577427 | 1481 | nuccore | 255045 |
| 6530 | Bradyrhizobium sp. BTA-1 partial 16S rRNA gene, strain BTA-1 | AJ558025 | 1481 | ena | 255045 |
Genome sequences
- @ref: 66792
- description: Bradyrhizobium canariense BTA-1
- accession: GCA_019402665
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 255045
GC content
- @ref: 6530
- GC-content: 63.8
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| flagellated | no | 68.963 | no |
| gram-positive | no | 98.183 | yes |
| anaerobic | no | 96.971 | yes |
| aerobic | yes | 91.093 | no |
| halophile | no | 95.618 | yes |
| spore-forming | no | 93.663 | yes |
| motile | yes | 86.804 | no |
| glucose-util | yes | 89.875 | yes |
| glucose-ferment | no | 90.626 | no |
| thermophile | no | 99.481 | yes |
External links
@ref: 6530
culture collection no.: DSM 16623, ATCC BAA 1002, CFNE 1008, LMG 22265
straininfo link
- @ref: 71458
- straininfo: 136005
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 15774626 | Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta. | Vinuesa P, Leon-Barrios M, Silva C, Willems A, Jarabo-Lorenzo A, Perez-Galdona R, Werner D, Martinez-Romero E | Int J Syst Evol Microbiol | 10.1099/ijs.0.63292-0 | 2005 | Bacterial Proteins, Bradyrhizobium/*classification/drug effects/growth & development, DNA, Ribosomal Spacer/analysis, Evolution, Molecular, Fabaceae/classification/*microbiology, Hydrogen-Ion Concentration, Molecular Sequence Data, N-Acetylglucosaminyltransferases/genetics, Nucleic Acid Hybridization, Oxidoreductases/genetics, Phenotype, Phylogeny, Sequence Analysis, DNA, Spain, *Symbiosis | Genetics |
| Phylogeny | 21257682 | Bradyrhizobium cytisi sp. nov., isolated from effective nodules of Cytisus villosus. | Chahboune R, Carro L, Peix A, Barrijal S, Velazquez E, Bedmar EJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.027649-0 | 2011 | Bacterial Proteins/genetics/metabolism, Bradyrhizobium/*classification/genetics/*isolation & purification/metabolism, Cytisus/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology | Metabolism |
| Phylogeny | 25609676 | Revision of the taxonomic status of the species Rhizobium lupini and reclassification as Bradyrhizobium lupini comb. nov. | Peix A, Ramirez-Bahena MH, Flores-Felix JD, Alonso de la Vega P, Rivas R, Mateos PF, Igual JM, Martinez-Molina E, Trujillo ME, Velazquez E | Int J Syst Evol Microbiol | 10.1099/ijs.0.000082 | 2015 | Bacterial Typing Techniques, Bradyrhizobium/*classification, DNA, Bacterial/genetics, Lupinus/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizobium/*classification, Sequence Analysis, DNA, Symbiosis | Genetics |
| Phylogeny | 31334752 | New chromosomal lineages within Microvirga and Bradyrhizobium genera nodulate Lupinus angustifolius growing on different Tunisian soils. | Rejili M, Msaddak A, Filali I, Benabderrahim MA, Mars M, Marin M | FEMS Microbiol Ecol | 10.1093/femsec/fiz118 | 2019 | Bacterial Proteins/genetics/metabolism, Bacterial Typing Techniques, Bradyrhizobium/classification/*genetics/isolation & purification/physiology, Chromosomes, Bacterial/genetics, DNA, Bacterial/genetics, Lupinus/*microbiology, Methylobacteriaceae/classification/*genetics/isolation & purification/physiology, Multilocus Sequence Typing, Phylogeny, *Plant Root Nodulation, Root Nodules, Plant/microbiology, Soil Microbiology, Symbiosis, Tunisia | Enzymology |
| Phylogeny | 32897848 | Bradyrhizobium hipponense sp. nov., isolated from Lupinus angustifolius growing in the northern region of Tunisia. | Rejili M, Off K, Brachmann A, Marin M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004445 | 2020 | Bacterial Typing Techniques, Base Composition, Bradyrhizobium/*classification, DNA, Bacterial/genetics, Genes, Bacterial, Lupinus/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Symbiosis, Tunisia | Transcriptome |
| 34625829 | The Fodder Legume Chamaecytisus albidus Establishes Functional Symbiosis with Different Bradyrhizobial Symbiovars in Morocco. | Bouhnik O, Alami S, Lamin H, Lamrabet M, Bennis M, Ouajdi M, Bellaka M, Antri SE, Abbas Y, Abdelmoumen H, Bedmar EJ, Idrissi MME | Microb Ecol | 10.1007/s00248-021-01888-4 | 2021 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 6530 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16623) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16623 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 23326 | Irina S. Kulichevskaya, Vladimir S. Guzev, Vladimir M. Gorlenko, Werner Liesack, Svetlana N. Dedysh | 10.1099/ijs.0.63962-0 | Rhodoblastus sphagnicola sp. nov., a novel acidophilic purple non-sulfur bacterium from Sphagnum peat bog | IJSEM 56: 1397-1402 2006 | 16738120 | |
| 23327 | Pablo Vinuesa, Milagros León-Barrios, Claudia Silva, Anne Willems, Adriana Jarabo-Lorenzo, Ricardo Pérez-Galdona, Dietrich Werner, Esperanza Martínez-Romero | 10.1099/ijs.0.63292-0 | Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies alpha and Bradyrhizobium genospecies beta | IJSEM 55: 569-575 2005 | 15774626 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 71458 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID136005.1 | StrainInfo: A central database for resolving microbial strain identifiers |