Strain identifier

BacDive ID: 18105

Type strain: Yes

Species: Exiguobacterium aurantiacum

Strain Designation: BL 77/1

Strain history: CIP <- 1989, NCIB <- B.M. Lund: strain BL 77/1

NCBI tax ID(s): 1397694 (strain), 33987 (species)

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General

@ref: 2534

BacDive-ID: 18105

DSM-Number: 6208

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Exiguobacterium aurantiacum BL 77/1 is an aerobe, mesophilic, motile bacterium that was isolated from alkaline effluent from potato washing.

NCBI tax id

NCBI tax idMatching level
33987species
1397694strain

strain history

@refhistory
2534<- NCDO <- M.D. Collins <- B.M. Lund, BL 77/1
116557CIP <- 1989, NCIB <- B.M. Lund: strain BL 77/1

doi: 10.13145/bacdive18105.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Exiguobacterium
  • species: Exiguobacterium aurantiacum
  • full scientific name: Exiguobacterium aurantiacum Collins et al. 1984

@ref: 2534

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacilli, not assigned to family

genus: Exiguobacterium

species: Exiguobacterium aurantiacum

full scientific name: Exiguobacterium aurantiacum Collins et al. 1984

strain designation: BL 77/1

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes93.643
69480100positive

colony morphology

@refincubation periodmedium usedcolony color
1972210-14 daysISP 2
1972210-14 daysISP 5
1972210-14 daysISP 6Honey yellow

multicellular morphology

@refforms multicellular complexmedium name
19722noISP 2
19722noISP 5
19722noISP 6

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2534EXIGUOBACTERIUM MEDIUM (DSMZ Medium 468)yeshttps://mediadive.dsmz.de/medium/468Name: EXIGUOBACTERIUM MEDIUM (DSMZ Medium 468) Composition: Nutrient broth No. 2 25.0 g/l Glucose 5.0 g/l Yeast extract 3.0 g/l Distilled water
19722ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19722ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19722ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
40617MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116557CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
2534positivegrowth28mesophilic
19722positiveoptimum28mesophilic
40617positivegrowth30mesophilic
56539positivegrowth25mesophilic
56539positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 56539
  • oxygen tolerance: aerobe

halophily

  • @ref: 19722
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

murein

  • @ref: 2534
  • murein short key: A11.01
  • type: A3alpha L-Lys-Gly

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1972217234glucose+
1972222599arabinose+
1972217992sucrose+
1972218222xylose+
1972217268myo-inositol-
1972229864mannitol-
1972228757fructose+
1972226546rhamnose+/-
1972216634raffinose-
1972262968cellulose-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19722-++-------+------+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19722+++-------+---+----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19722---------++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
2534alkaline effluent from potato washingUnited KingdomGBREurope
56539Alkaline effluent from potato washing
116557Other, Effluent of a potato processing factory

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Wastewater
#Host#Plants#Herbaceous plants (Grass,Crops)
#Condition#Alkaline

taxonmaps

  • @ref: 69479
  • File name: preview.99_1230.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_130;96_715;97_837;98_973;99_1230&stattab=map
  • Last taxonomy: Exiguobacterium aurantiacum
  • 16S sequence: DQ019166
  • Sequence Identity:
  • Total samples: 4579
  • soil counts: 374
  • aquatic counts: 1593
  • animal counts: 2195
  • plant counts: 417

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
25341Risk group (German classification)
197221Risk group (German classification)
1165571Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Exiguobacterium aurantiacum strain DSM 6208 16S ribosomal RNA gene, partial sequenceDQ0191661552ena33987
20218Exiguobacterium aurantiacum gene for 16S rRNA, partial sequence, strain: NBRC 14763AB6806571487ena33987
20218E.aurantiacum (NCDO 2321) 16S rRNAX703161527ena33987

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Exiguobacterium aurantiacum DSM 6208GCA_000702585contigncbi1397694
66792Exiguobacterium aurantiacum DSM 62081397694.4wgspatric1397694
66792Exiguobacterium aurantiacum DSM 62082562617001draftimg1397694

GC content

  • @ref: 2534
  • GC-content: 53.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes80no
motileyes91.208no
gram-positiveyes90.635no
anaerobicno98.947no
halophileyes73.182yes
spore-formingno68.632no
glucose-utilyes88.485yes
thermophileno99.331no
aerobicyes71.062no
flagellatedyes69.665no
glucose-fermentno81.421yes

External links

@ref: 2534

culture collection no.: DSM 6208, ATCC 35652, NCDO 2321, NCIB 11798, CCUG 44910, NCIMB 11798, IFO 14763, CIP 103353

straininfo link

  • @ref: 87093
  • straininfo: 368693

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology9804993Molecular characterization of guanosine kinase gene from a facultative alkalophile, Exiguobacterium aurantiacum ATCC 35652.Usuda Y, Kawasaki H, Shimaoka M, Utagawa TBiochim Biophys Acta10.1016/s0167-4781(98)00166-31998Amino Acid Sequence, Base Sequence, Cloning, Molecular, Escherichia coli, Gram-Positive Bacteria/*enzymology/*genetics, Hydrogen-Ion Concentration, Molecular Sequence Data, Molecular Weight, Phosphotransferases (Alcohol Group Acceptor)/biosynthesis/chemistry/*genetics, Recombinant Proteins/biosynthesis/chemistry, Sequence Alignment, Sequence Homology, Amino Acid, Sequence Homology, Nucleic AcidGenetics
Phylogeny12148624Exiguobacterium undae sp. nov. and Exiguobacterium antarcticum sp. nov.Fruhling A, Schumann P, Hippe H, Straubler B, Stackebrandt EInt J Syst Evol Microbiol10.1099/00207713-52-4-11712002Bacillaceae/chemistry/*classification/genetics/physiology, Bacterial Typing Techniques, DNA, Ribosomal, Fresh Water/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Pigments, Biological/metabolism, RNA, Ribosomal, 16S, Ribotyping, Sequence Analysis, DNA, Spectroscopy, Fourier Transform InfraredGenetics
Phylogeny16564954Exiguobacterium mexicanum sp. nov. and Exiguobacterium artemiae sp. nov., isolated from the brine shrimp Artemia franciscana.Lopez-Cortes A, Schumann P, Pukall R, Stackebrandt ESyst Appl Microbiol10.1016/j.syapm.2005.09.0072005Animals, Artemia/*microbiology, Bacillaceae/classification/genetics/*isolation & purification, Phenotype, RNA, Ribosomal, 16S/chemistryPhenotype
Phylogeny23229437Exiguobacterium himgiriensis sp. nov. a novel member of the genus Exiguobacterium, isolated from the Indian Himalayas.Singh NK, Raichand R, Kaur I, Kaur C, Pareek S, Mayilraj SAntonie Van Leeuwenhoek10.1007/s10482-012-9861-52012Bacillales/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, India, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Pigments, Biological/metabolism, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny23838447Exiguobacterium alkaliphilum sp. nov. isolated from alkaline wastewater drained sludge of a beverage factory.Mohan Kulshreshtha N, Kumar R, Begum Z, Shivaji S, Kumar AInt J Syst Evol Microbiol10.1099/ijs.0.039123-02013Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, *Beverages, DNA, Bacterial/genetics, Fatty Acids/chemistry, Food Industry, India, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sewage/microbiology, Vitamin K 2/analogs & derivatives/chemistry, Waste Water/*microbiologyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2534Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6208)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6208
19722Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM6208.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40617Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15141
56539Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44910)https://www.ccug.se/strain?id=44910
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
87093Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID368693.1StrainInfo: A central database for resolving microbial strain identifiers
116557Curators of the CIPCollection of Institut Pasteur (CIP 103353)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103353