Strain identifier

BacDive ID: 181

Type strain: Yes

Species: Schaalia funkei

Strain Designation: Hudd B9912678

Strain history: CIP <- 2000, CCUG <- 1999, Jakalas/Vincent, PHLS, Huddinge, Sweden

NCBI tax ID(s): 132933 (species)

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General

@ref: 5986

BacDive-ID: 181

DSM-Number: 15537

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped

description: Schaalia funkei Hudd B9912678 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from human blood, endocarditis.

NCBI tax id

  • NCBI tax id: 132933
  • Matching level: species

strain history

@refhistory
5986<- CCUG <- V. Jalakas, PHLS, Sweden
67771<- CCUG <- V Jalakas, PHLS, Huddinge, Sweden
119495CIP <- 2000, CCUG <- 1999, Jakalas/Vincent, PHLS, Huddinge, Sweden

doi: 10.13145/bacdive181.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Schaalia
  • species: Schaalia funkei
  • full scientific name: Schaalia funkei (Lawson et al. 2001) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces funkei

@ref: 5986

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Schaalia

species: Schaalia funkei

full scientific name: Schaalia funkei (Lawson et al. 2001) Nouioui et al. 2018

strain designation: Hudd B9912678

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
23117positiverod-shaped
67771rod-shaped
67771curved-shaped
67771positive
119495positiverod-shapedno

colony morphology

@reftype of hemolysiscolony sizecolony colorincubation periodmedium used
23117gamma<1.0 mmgrey1 dayColumbia blood agar
119495

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5986FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)yeshttps://mediadive.dsmz.de/medium/1203Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water
5986COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
23117Columbia blood agaryes
36419MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
119495CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5986positivegrowth37mesophilic
36419positivegrowth37mesophilic
55553positivegrowth37mesophilic
67771positivegrowth37mesophilic
119495positivegrowth30-41
119495nogrowth10psychrophilic
119495nogrowth25mesophilic
119495nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5986microaerophile
23117facultative anaerobe
55553anaerobe
55553microaerophile
67771microaerophile
119495facultative anaerobe

spore formation

@refspore formation
23117no
67771no

halophily

@refsaltgrowthtested relationconcentration
119495NaClpositivegrowth0-6 %
119495NaClnogrowth8 %
119495NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2311723456cyclodextrin-builds acid from
2311718333D-arabitol-builds acid from
2311728087glycogen-builds acid from
2311730849L-arabinose-builds acid from
2311729864mannitol-builds acid from
231176731melezitose-builds acid from
2311728053melibiose-builds acid from
23117320055methyl beta-D-glucopyranoside-builds acid from
2311727941pullulan-builds acid from
2311716634raffinose-builds acid from
2311730911sorbitol-builds acid from
2311733954tagatose-builds acid from
2311727082trehalose-builds acid from
231174853esculin-hydrolysis
231175291gelatin-hydrolysis
2311728017starch-hydrolysis
2311715688acetoin-other
2311765327D-xylose+builds acid from
2311717234glucose+builds acid from
2311717992sucrose+builds acid from
23117606565hippurate+hydrolysis
2311717716lactose+/-builds acid from
2311717306maltose+/-builds acid from
2311733942ribose+/-builds acid from
2311717632nitrate+/-reduction
11949516947citrate-carbon source
1194954853esculin-hydrolysis
119495606565hippurate+hydrolysis
11949517632nitrate+reduction
11949516301nitrite-reduction
11949517632nitrate+respiration
6837917632nitrate+reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose+fermentation
6837965327D-xylose+fermentation
6837916899D-mannitol-fermentation
6837917306maltose+fermentation
6837917716lactose+fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source
6838129016arginine-hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose-builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate+hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose-builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

antibiotic resistance

  • @ref: 119495
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
11949535581indoleno
6838035581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6838115688acetoin-
6838035581indole-
11949515688acetoin-
11949517234glucose-

enzymes

@refvalueactivityec
23117acid phosphatase+3.1.3.2
23117alkaline phosphatase+3.1.3.1
23117alpha-fucosidase-3.2.1.51
23117alpha-galactosidase-3.2.1.22
23117alpha-glucosidase+3.2.1.20
23117alpha-mannosidase-3.2.1.24
23117arginine dihydrolase-3.5.3.6
23117beta-galactosidase+/-3.2.1.23
23117beta-glucosidase-3.2.1.21
23117beta-glucuronidase-3.2.1.31
23117beta-mannosidase-3.2.1.25
23117catalase-1.11.1.6
23117chymotrypsin-3.4.4.5
23117cystine arylamidase+3.4.11.3
23117esterase (C 4)-
23117esterase lipase (C 8)-
23117glycyl tryptophan arylamidase-
23117leucine arylamidase+3.4.11.1
23117lipase (C 14)-
23117N-acetyl-beta-glucosaminidase+/-3.2.1.52
23117pyrazinamidase+/-3.5.1.B15
23117pyroglutamic acid arylamidase-
23117pyrrolidonyl arylamidase-3.4.19.3
23117tripeptide aminopeptidase+3.4.11.4
23117trypsin-3.4.21.4
23117urease-3.5.1.5
23117valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382alkaline phosphatase+3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5
68379catalase-1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
119495oxidase-
119495beta-galactosidase+3.2.1.23
119495alcohol dehydrogenase-1.1.1.1
119495gelatinase-
119495caseinase-3.4.21.50
119495catalase-1.11.1.6
119495gamma-glutamyltransferase+2.3.2.2
119495lysine decarboxylase-4.1.1.18
119495ornithine decarboxylase-4.1.1.17
119495phenylalanine ammonia-lyase-4.3.1.24
119495tryptophan deaminase-
119495urease-3.5.1.5

fatty acid profile

  • @reffatty acidpercentageECL
    55553C14:02.114
    55553C16:026.116
    55553C18:015.818
    55553C16:1 ω7c2.415.819
    55553C18:1 ω9c2217.769
    55553C18:2 ω6,9c/C18:0 ANTE31.717.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG
  • @reffatty acidpercentageECL
    55553C14:0114
    55553C16:02316
    55553C17:0117
    55553C18:024.518
    55553C16:1 ω7c1.315.819
    55553C18:1 ω9c20.517.769
    55553C18:2 ω6,9c/C18:0 ANTE28.817.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
55553++-+-+++----+++-+++--

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
55553-+---+-+--+----+-+--
119495-+++-+-+--++-+-+-+--

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
55553-+-+-+---++-----+++-++-++++-+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
55553--+--+------+----++---+---------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
5986human blood, endocarditisHuddingeSwedenSWEEurope
2311740-year-old female intravenous drug user with Staphylococcus aureus endocarditis
55553Human blood,endocarditis,clinically documentedHuddingeSwedenSWEEurope1999-09-21
67771From human blood, endocarditis, clinically documentedHuddingeSwedenSWEEurope
119495Human, BloodHuddingeSwedenSWEEurope1999

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Host#Human#Female
#Infection#Inflammation
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_46871.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_224;96_15710;97_26614;98_33846;99_46871&stattab=map
  • Last taxonomy: Actinomyces
  • 16S sequence: NR_028960
  • Sequence Identity:
  • Total samples: 1276
  • soil counts: 9
  • aquatic counts: 62
  • animal counts: 1196
  • plant counts: 9

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
59862Risk group (German classification)
1194952Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5986Actinomyces funkeii partial 16S rRNA gene, strain CCUG 42773AJ4048891395ena132933
67771Schaalia funkei strain CCUG 42773 16S ribosomal RNA, partial sequenceNR_0289601366nuccore132933

External links

@ref: 5986

culture collection no.: DSM 15537, CCUG 42773, CIP 106713, KCTC 15233

straininfo link

  • @ref: 69863
  • straininfo: 87791

literature

  • topic: Phylogeny
  • Pubmed-ID: 11411706
  • title: Actinomyces funkei sp. nov., isolated from human clinical specimens.
  • authors: Lawson PA, Nikolaitchouk N, Falsen E, Westling K, Collins MD
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-3-853
  • year: 2001
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Adult, Cefuroxime/therapeutic use, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Drug Therapy, Combination/therapeutic use, Endocarditis, Bacterial/complications/drug therapy, Female, Humans, Molecular Sequence Data, Phenotype, *Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Staphylococcal Infections/complications/drug therapy, Substance Abuse, Intravenous/complications
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5986Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15537)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15537
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23117P A Lawson, N Nikolaitchouk, E Falsen, K Westling, M D Collins10.1099/00207713-51-3-853Actinomyces funkei sp. nov., isolated from human clinical specimens.IJSEM 51: 853-855 200111411706
36419Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18874
55553Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 42773)https://www.ccug.se/strain?id=42773
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68379Automatically annotated from API Coryne
68380Automatically annotated from API rID32A
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69863Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID87791.1StrainInfo: A central database for resolving microbial strain identifiers
119495Curators of the CIPCollection of Institut Pasteur (CIP 106713)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106713