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Strain identifier

BacDive ID: 18089

Type strain: Yes

Species: Acetatifactor muris

Strain Designation: CT-m2

Strain history: <- T. Clavel, BFLM, TU Munich, Freising-Weihenstephan, Germany; CT-m2 <- T. Clavel {2009}

NCBI tax ID(s): 879566 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 17334

BacDive-ID: 18089

DSM-Number: 23669

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Acetatifactor muris CT-m2 is an anaerobe, mesophilic bacterium that was isolated from cecal content of obese mouse.

NCBI tax id

  • NCBI tax id: 879566
  • Matching level: species

strain history: <- T. Clavel, BFLM, TU Munich, Freising-Weihenstephan, Germany; CT-m2 <- T. Clavel {2009}

doi: 10.13145/bacdive18089.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Acetatifactor
  • species: Acetatifactor muris
  • full scientific name: Acetatifactor muris Pfeiffer et al. 2013

@ref: 17334

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Lachnospiraceae

genus: Acetatifactor

species: Acetatifactor muris

full scientific name: Acetatifactor muris Pfeiffer et al. 2013

strain designation: CT-m2

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 94.096

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17334WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) (DSMZ Medium 339a)yeshttps://mediadive.dsmz.de/medium/339aName: WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) (DSMZ Medium 339a) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l D-Glucose 5.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
17334PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104; with strain-specific modifications) Composition: Yeast extract 10.0 g/l Trypticase peptone 5.0 g/l Peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Vitamin K1 NaOH Tween 80 Clarified rumen fluid Distilled water

culture temp

  • @ref: 17334
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
17334anaerobe
69480anaerobe99.998

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 61.141

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 99.779

Isolation, sampling and environmental information

isolation

  • @ref: 17334
  • sample type: cecal content of obese mouse
  • geographic location: Freising-Weihenstephan
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body Product#Gastrointestinal tract#Caecal content

taxonmaps

  • @ref: 69479
  • File name: preview.99_15629.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1091;96_7982;97_9602;98_11794;99_15629&stattab=map
  • Last taxonomy: Acetatifactor muris subclade
  • 16S sequence: HM989805
  • Sequence Identity:
  • Total samples: 13512
  • soil counts: 62
  • aquatic counts: 44
  • animal counts: 13400
  • plant counts: 6

Safety information

risk assessment

  • @ref: 17334
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17334
  • description: Acetatifactor muris strain CT-m2 16S ribosomal RNA gene, partial sequence
  • accession: HM989805
  • length: 1376
  • database: ena
  • NCBI tax ID: 879566

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acetatifactor muris DSM 23669GCA_024623325contigncbi879566
66792Flavobacteria bacterium BBFL7 BBFL7GCA_000153385scaffoldpatric156586

GC content

  • @ref: 17334
  • GC-content: 48.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 17334

culture collection no.: DSM 23669, ATCC BAA 2170

straininfo link

@refpassport
20218http://www.straininfo.net/strains/884033
20218http://www.straininfo.net/strains/884032

literature

  • topic: Phylogeny
  • Pubmed-ID: 22659832
  • title: Acetatifactor muris gen. nov., sp. nov., a novel bacterium isolated from the intestine of an obese mouse.
  • authors: Pfeiffer N, Desmarchelier C, Blaut M, Daniel H, Haller D, Clavel T
  • journal: Arch Microbiol
  • DOI: 10.1007/s00203-012-0822-1
  • year: 2012
  • mesh: Animals, Genotype, Gram-Positive Bacteria/*classification/genetics/isolation & purification, Intestines/*microbiology, Male, Mice, Mice, Inbred C57BL, Mice, Obese, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17334Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23669)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23669
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)