Strain identifier

BacDive ID: 18073

Type strain: Yes

Species: Blautia hydrogenotrophica

Strain Designation: S5a33

Strain history: DSM 10507 <-- A. Bernalier S5a33.

NCBI tax ID(s): 476272 (strain), 53443 (species)

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General

@ref: 3952

BacDive-ID: 18073

DSM-Number: 10507

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive

description: Blautia hydrogenotrophica S5a33 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from human feces.

NCBI tax id

NCBI tax idMatching level
53443species
476272strain

strain history

@refhistory
3952<- A. Bernalier, S5a33
67770DSM 10507 <-- A. Bernalier S5a33.

doi: 10.13145/bacdive18073.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Blautia
  • species: Blautia hydrogenotrophica
  • full scientific name: Blautia hydrogenotrophica (Bernalier et al. 1997) Liu et al. 2008

  • synonyms

    • @ref: 20215
    • synonym: Ruminococcus hydrogenotrophicus

@ref: 3952

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Blautia

species: Blautia hydrogenotrophica

full scientific name: Blautia hydrogenotrophica (Bernalier et al. 1997) Liu et al. 2008

strain designation: S5a33

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

Culture and growth conditions

culture medium

  • @ref: 3952
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
3952positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
3952anaerobe
69480anaerobe99.999

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.453

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
3952-----++----------------------
3952-----+-----------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3952human fecesFranceFRAEurope
67770Human feces

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_3985.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_2024;97_2439;98_3017;99_3985&stattab=map
  • Last taxonomy: Blautia hydrogenotrophica subclade
  • 16S sequence: X95624
  • Sequence Identity:
  • Total samples: 31330
  • soil counts: 83
  • aquatic counts: 791
  • animal counts: 30351
  • plant counts: 105

Safety information

risk assessment

  • @ref: 3952
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
3952Blautia hydrogenotrophica 16S rRNA gene, strain S5a36X956241458ena53443
67770Blautia hydrogenotrophica gene for 16S ribosomal RNA, partial sequenceAB9107511495ena53443

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Blautia hydrogenotrophica DSM 10507476272.21wgspatric476272
66792Blautia hydrogenotrophica DSM 10507643886199draftimg476272
67770Blautia hydrogenotrophica DSM 10507GCA_000157975scaffoldncbi476272

GC content

@refGC-contentmethod
395245high performance liquid chromatography (HPLC)
6777045.2high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno52no
gram-positiveyes84.292no
anaerobicyes99.545no
halophileno78.692no
spore-formingno54.064no
glucose-utilyes89.433no
aerobicno98.788no
thermophileno98.618yes
motileno89.503no
flagellatedno94.175no
glucose-fermentyes78.934no

External links

@ref: 3952

culture collection no.: DSM 10507, JCM 14656

straininfo link

  • @ref: 87061
  • straininfo: 48838

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8703194Ruminococcus hydrogenotrophicus sp. nov., a new H2/CO2-utilizing acetogenic bacterium isolated from human feces.Bernalier A, Willems A, Leclerc M, Rochet V, Collins MDArch Microbiol10.1007/s0020300503731996Bacteria/growth & development/*isolation & purification/metabolism, Base Sequence, Carbon Dioxide/*metabolism, Feces/*microbiology, Fermentation, Humans, Hydrogen/*metabolism, Molecular Sequence Data, PhylogenyMetabolism
Metabolism30322445Integrated culturing, modeling and transcriptomics uncovers complex interactions and emergent behavior in a three-species synthetic gut community.D'hoe K, Vet S, Faust K, Moens F, Falony G, Gonze D, Llorens-Rico V, Gelens L, Danckaert J, De Vuyst L, Raes JElife10.7554/eLife.370902018Bacteria/metabolism, Cells, Cultured, Computer Simulation, Fermentation, Formates/metabolism, Fructose/metabolism, Gastrointestinal Microbiome/*genetics, Gene Expression Regulation, Bacterial, Humans, Kinetics, Metabolome/genetics, Models, Biological, Prokaryotic Cells/metabolism, RNA, Ribosomal, 16S/genetics, Species Specificity, Transcriptome/*geneticsPhylogeny
Phylogeny32100703Blautia faecicola sp. nov., isolated from faeces from a healthy human.Kim JS, Park JE, Lee KC, Choi SH, Oh BS, Yu SY, Eom MK, Kang SW, Han KI, Suh MK, Lee DH, Yoon H, Kim BY, Yang SJ, Lee JH, Lee JS, Park SHInt J Syst Evol Microbiol10.1099/ijsem.0.0040152020Acetic Acid/metabolism, Bacterial Typing Techniques, Base Composition, Clostridiales/*classification/isolation & purification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Feces/*microbiology, Fermentation, Humans, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNAPathogenicity

Reference

@idauthorscataloguedoi/urltitle
3952Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10507)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10507
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
87061Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48838.1StrainInfo: A central database for resolving microbial strain identifiers