Strain identifier

BacDive ID: 18068

Type strain: Yes

Species: Blautia producta

Strain Designation: 2396

Strain history: <- DSM <- ATCC <- WEC Moore, VPI 4299 <- A Prevot, 2396

NCBI tax ID(s): 1121114 (strain), 33035 (species)

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General

@ref: 1258

BacDive-ID: 18068

DSM-Number: 2950

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive

description: Blautia producta 2396 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from human septicemia.

NCBI tax id

NCBI tax idMatching level
1121114strain
33035species

strain history

@refhistory
1258<- ATCC <- W.E.C. Moore, VPI 4299 <- A. Prévot, 2396
67770T. Mitsuoka S10-45.
67771<- DSM <- ATCC <- WEC Moore, VPI 4299 <- A Prevot, 2396

doi: 10.13145/bacdive18068.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Blautia
  • species: Blautia producta
  • full scientific name: Blautia producta (Prévot 1941) Liu et al. 2008

  • synonyms

    @refsynonym
    20215Streptococcus productus
    20215Ruminococcus productus
    20215Peptostreptococcus productus

@ref: 1258

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Blautia

species: Blautia producta

full scientific name: Blautia producta (Prévot 1941) Liu et al. 2008

strain designation: 2396

type strain: yes

Morphology

cell morphology

@refgram stainconfidence
67771positive
69480positive100

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1258PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
1258CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

culture temp

@refgrowthtypetemperaturerange
1258positivegrowth37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
1258anaerobe
67771anaerobe
69480anaerobe99.531

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 98.696

murein

  • @ref: 1258
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837516199urea-hydrolysis
6837529016arginine-hydrolysis
6837518257ornithine+degradation
683754853esculin+hydrolysis
6837517634D-glucose-fermentation
6837515824D-fructose+fermentation
6837516024D-mannose+fermentation
6837517306maltose+fermentation
6837517716lactose-fermentation
6837527082trehalose-fermentation
6837516899D-mannitol-fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate-reduction
6837517992sucrose-fermentation
6837559640N-acetylglucosamine-fermentation
6837532528turanose-fermentation
6837522599arabinose-fermentation
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is sensitive: yes
  • sensitivity conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6838035581indole-
6837515688acetoin-

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380alpha-arabinosidase+3.2.1.55
68380beta-glucosidase+3.2.1.21
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase-3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase+3.2.1.21
68375ornithine decarboxylase+4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
1258--++-+++------------------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
1258--++--+++--------------------
1258--++-+++---+/------------------
1258--++-+++---------------------

Isolation, sampling and environmental information

isolation

@refsample type
1258human septicemia
67770Human feces
67771From human septicemia

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Human
#Host Body Product#Fluids#Blood

taxonmaps

  • @ref: 69479
  • File name: preview.99_27878.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_1360;97_1614;98_1963;99_27878&stattab=map
  • Last taxonomy: Blautia
  • 16S sequence: X94966
  • Sequence Identity:
  • Total samples: 5135
  • soil counts: 27
  • aquatic counts: 130
  • animal counts: 4973
  • plant counts: 5

Safety information

risk assessment

  • @ref: 1258
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Blautia producta gene for 16S ribosomal RNA, partial sequenceAB6009981493ena1121114
20218Ruminococcus productus small subunit ribosomal RNA (16S rDNA) geneL765951427ena1121114
1258Ruminococcus productus gene for 16S ribosomal RNA, partial sequenceD141441381ena1121114
1258P.productus 16S ribosomal RNAX949661482ena1121114

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Blautia producta DSM 2950GCA_014131715completencbi33035
66792Blautia producta ATCC 27340 = DSM 2950 JCM 1471GCA_010669205completencbi1121114
66792Blautia producta ATCC 273401121114.4wgspatric1121114
66792Blautia producta ATCC 273401121114.7wgspatric1121114
66792Blautia producta ATCC 27340 = DSM 2950 strain JCM 14711121114.32completepatric1121114
66792Blautia producta strain DSM 295033035.113completepatric33035
66792Blautia producta DSM 29502524023161draftimg1121114
66792Blautia producta ATCC 273402515154176draftimg1121114
67770Blautia producta ATCC 27340 = DSM 2950GCA_000439125scaffoldncbi1121114
67771Blautia producta ATCC 27340 = DSM 2950GCA_000373885scaffoldncbi1121114

GC content

  • @ref: 1258
  • GC-content: 45.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes61no
flagellatedno93.517no
gram-positiveyes87.612yes
anaerobicyes97.75no
aerobicno96.373no
halophileno88.58no
spore-formingyes71.321no
thermophileno98.469no
glucose-utilyes92.645no
motileno85.194no
glucose-fermentyes69.952no

External links

@ref: 1258

culture collection no.: DSM 2950, ATCC 27340, CCUG 10976, CCUG 9990, JCM 1471, KCTC 15607, VPI 4299, NCTC 11829, KCTC 3242, KCTC 3695

straininfo link

  • @ref: 87056
  • straininfo: 38945

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism1521774Comparative evaluation of the metabolic potentials of different strains of Peptostreptococcus productus: utilization and transformation of aromatic compounds.Parekh M, Keith ES, Daniel SL, Drake HLFEMS Microbiol Lett10.1016/0378-1097(92)90585-c1992Acrylates/metabolism, Benzoates/metabolism, Benzyl Alcohols/metabolism, Biotransformation, Peptostreptococcus/growth & development/*metabolism, Phenols/metabolism
Pathogenicity2685068Serological studies of peptostreptococci using an indirect fluorescent antibody test.Collins ML, Falkler WA Jr, Hall ER, Graham MBJ Dent Res10.1177/002203458906801108011989Fluorescent Antibody Technique, Humans, Mouth Diseases/microbiology, Peptostreptococcus/*isolation & purificationEnzymology
Phylogeny812355616S ribosomal DNA sequences of anaerobic cocci and proposal of Ruminococcus hansenii comb. nov. and Ruminococcus productus comb. nov.Ezaki T, Li N, Hashimoto Y, Miura H, Yamamoto HInt J Syst Bacteriol10.1099/00207713-44-1-1301994Anaerobiosis, Base Sequence, DNA, Bacterial/*chemistry, DNA, Ribosomal/*chemistry, Molecular Sequence Data, Peptococcaceae/*classification/*genetics, RNA, Ribosomal, 16S/*geneticsGenetics
Pathogenicity16233782Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota.Minamida K, Shiga K, Sujaya IN, Sone T, Yokota A, Hara H, Asano K, Tomita FJ Biosci Bioeng10.1263/jbb.99.2302005Administration, Oral, Animals, Bacteroides/*drug effects/isolation & purification, Cecum/*drug effects/*microbiology, Clostridium/*drug effects, Dietary Supplements/microbiology, Disaccharides/*administration & dosage, Gastrointestinal Contents/*microbiology, Male, Rats, Rats, Sprague-Dawley, Ruminococcus/*drug effects/isolation & purificationEnzymology
Phylogeny21571940Blautia stercoris sp. nov., isolated from human faeces.Park SK, Kim MS, Roh SW, Bae JWInt J Syst Evol Microbiol10.1099/ijs.0.031625-02011Acetic Acid/chemistry, Adult, Bacteria, Anaerobic/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Fermentation, Genotype, Gram-Positive Bacteria/*classification/genetics/isolation & purification, Humans, Male, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNAGenetics
Phylogeny30407903Blautia argi sp. nov., a new anaerobic bacterium isolated from dog faeces.Paek J, Shin Y, Kook JK, Chang YHInt J Syst Evol Microbiol10.1099/ijsem.0.0029812018Animals, Bacterial Typing Techniques, Base Composition, Clostridiales/*classification/isolation & purification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Dogs/*microbiology, Fatty Acids/chemistry, Feces/*microbiology, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Metabolism31177942The 'in vivo lifestyle' of bile acid 7alpha-dehydroxylating bacteria: comparative genomics, metatranscriptomic, and bile acid metabolomics analysis of a defined microbial community in gnotobiotic mice.Ridlon JM, Devendran S, Alves JM, Doden H, Wolf PG, Pereira GV, Ly L, Volland A, Takei H, Nittono H, Murai T, Kurosawa T, Chlipala GE, Green SJ, Hernandez AG, Fields CJ, Wright CL, Kakiyama G, Cann I, Kashyap P, McCracken V, Gaskins HRGut Microbes10.1080/19490976.2019.16181732019Animals, Bacteria, Anaerobic/*genetics/*metabolism, Bacteroides/genetics/metabolism, Bile Acids and Salts/*metabolism, Bilophila/genetics/metabolism, Cecum/*microbiology, Cholic Acids/metabolism, Clostridium/genetics/metabolism, Gene Expression Regulation, Bacterial, Genome, Bacterial, Genomics, Germ-Free Life, Humans, *Metabolome, Mice, Mice, Inbred C57BL, Microbiota, Operon, RNA-Seq, *Transcriptome, Up-RegulationGenetics
Phylogeny32100703Blautia faecicola sp. nov., isolated from faeces from a healthy human.Kim JS, Park JE, Lee KC, Choi SH, Oh BS, Yu SY, Eom MK, Kang SW, Han KI, Suh MK, Lee DH, Yoon H, Kim BY, Yang SJ, Lee JH, Lee JS, Park SHInt J Syst Evol Microbiol10.1099/ijsem.0.0040152020Acetic Acid/metabolism, Bacterial Typing Techniques, Base Composition, Clostridiales/*classification/isolation & purification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Feces/*microbiology, Fermentation, Humans, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNAPathogenicity
Genetics32327523Complete Genome Sequence of Blautia producta JCM 1471(T).Tourlousse DM, Sakamoto M, Miura T, Narita K, Ohashi A, Uchino Y, Yamazoe A, Kameyama K, Terauchi J, Ohkuma M, Kawasaki H, Sekiguchi YMicrobiol Resour Announc10.1128/MRA.00141-202020
33922843A Comprehensive Assessment of the Safety of Blautia producta DSM 2950.Liu X, Guo W, Cui S, Tang X, Zhao J, Zhang H, Mao B, Chen WMicroorganisms10.3390/microorganisms90509082021
Metabolism35328413Biochemical Basis of Xylooligosaccharide Utilisation by Gut Bacteria.Singh RP, Bhaiyya R, Thakur R, Niharika J, Singh C, Latousakis D, Saalbach G, Nepogodiev SA, Singh P, Sharma SC, Sengupta S, Juge N, Field RAInt J Mol Sci10.3390/ijms230629922022Bacteria/genetics/metabolism, Glucuronates, Humans, Oligosaccharides, Phylogeny, Substrate Specificity, *Xylans/metabolism, *Xylosidases/metabolismPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1258Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2950)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2950
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68375Automatically annotated from API ID32STA
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
87056Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38945.1StrainInfo: A central database for resolving microbial strain identifiers