Strain identifier

BacDive ID: 18049

Type strain: Yes

Species: Thermoanaerobacterium thermosulfurigenes

Strain Designation: 4B

Strain history: <- J. G. Zeikus, University of Wisconsin, Department of Bacteriology, Madison, Wisconsin, USA; 4B (Clostridium thermosulfurigenes)

NCBI tax ID(s): 33950 (species)

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General

@ref: 998

BacDive-ID: 18049

DSM-Number: 2229

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, alcohol production

description: Thermoanaerobacterium thermosulfurigenes 4B is an anaerobe, thermophilic bacterium that produces alcohol and was isolated from thermal spring.

NCBI tax id

  • NCBI tax id: 33950
  • Matching level: species

strain history

  • @ref: 998
  • history: <- J. G. Zeikus, University of Wisconsin, Department of Bacteriology, Madison, Wisconsin, USA; 4B (Clostridium thermosulfurigenes)

doi: 10.13145/bacdive18049.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermoanaerobacterales
  • family: Thermoanaerobacteraceae
  • genus: Thermoanaerobacterium
  • species: Thermoanaerobacterium thermosulfurigenes
  • full scientific name: Thermoanaerobacterium thermosulfurigenes (Schink and Zeikus 1983) Lee et al. 1993
  • synonyms

    @refsynonym
    20215Clostridium thermosulfurogenes
    20215Clostridium thermosulfurigenes

@ref: 998

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Thermoanaerobacterium

species: Thermoanaerobacterium thermosulfurigenes

full scientific name: Thermoanaerobacterium thermosulfurigenes (Schink and Zeikus 1983) Lee et al. 1993

strain designation: 4B

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 998
  • name: THERMOANAEROBACTERIUM MEDIUM (DSMZ Medium 211)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/211
  • composition: Name: THERMOANAEROBACTERIUM MEDIUM (DSMZ Medium 211) Composition: Na2HPO4 x 12 H2O 5.21141 g/l D-Glucose 4.91642 g/l NH4Cl 0.983284 g/l Yeast extract 0.983284 g/l Na2S x 9 H2O 0.491642 g/l KH2PO4 0.294985 g/l MgCl2 x 6 H2O 0.196657 g/l Nitrilotriacetic acid 0.12586 g/l NaCl 0.00983284 g/l FeCl2 x 4 H2O 0.00196657 g/l CoCl2 x 6 H2O 0.00167158 g/l FeSO4 x 7 H2O 0.00147493 g/l MnCl2 x 4 H2O 0.000983284 g/l ZnCl2 0.000983284 g/l CaCl2 x 2 H2O 0.000983284 g/l Sodium resazurin 0.000491642 g/l Na2SeO3 x 5 H2O 0.000294985 g/l NiCl2 x 6 H2O 0.000294985 g/l CuCl2 0.000196657 g/l Na2MoO4 x 2 H2O 9.83284e-05 g/l H3BO3 9.83284e-05 g/l Pyridoxine hydrochloride 4.91642e-05 g/l Calcium D-(+)-pantothenate 2.45821e-05 g/l p-Aminobenzoic acid 2.45821e-05 g/l (DL)-alpha-Lipoic acid 2.45821e-05 g/l Nicotinic acid 2.45821e-05 g/l Riboflavin 2.45821e-05 g/l Thiamine HCl 2.45821e-05 g/l Folic acid 9.83284e-06 g/l Biotin 9.83284e-06 g/l Vitamin B12 4.91642e-07 g/l Distilled water

culture temp

  • @ref: 998
  • growth: positive
  • type: growth
  • temperature: 60
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 998
  • oxygen tolerance: anaerobe

compound production

@refcompound
998sulfur from thiosulfate
998pectin methylesterase
998polygalacturonate hydrolase
998ethanol
998ß amylase, thermostable

Isolation, sampling and environmental information

isolation

  • @ref: 998
  • sample type: thermal spring
  • geographic location: Wyoming, Yellowstone Natl. Park, Octopus Spring
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_1467.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15961;96_838;97_980;98_1157;99_1467&stattab=map
  • Last taxonomy: Thermoanaerobacterium xylanolyticum
  • 16S sequence: L09171
  • Sequence Identity:
  • Total samples: 1780
  • soil counts: 45
  • aquatic counts: 83
  • animal counts: 1638
  • plant counts: 14

Safety information

risk assessment

  • @ref: 998
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 998
  • description: Thermoanaerobacterium thermosulfurigenes strain 4BT 16S ribosomal RNA gene, partial sequence
  • accession: L09171
  • length: 1574
  • database: ena
  • NCBI tax ID: 33950

Genome sequences

  • @ref: 66792
  • description: Thermoanaerobacterium thermosulfurigenes DSM 2229
  • accession: 2861644854
  • assembly level: draft
  • database: img
  • NCBI tax ID: 33950

GC content

  • @ref: 998
  • GC-content: 32.6

External links

@ref: 998

culture collection no.: DSM 2229, ATCC 33743

straininfo link

  • @ref: 87038
  • straininfo: 47552

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1854207alpha-Amylase of Clostridium thermosulfurogenes EM1: nucleotide sequence of the gene, processing of the enzyme, and comparison of other alpha-amylases.Bahl H, Burchhardt G, Spreinat A, Haeckel K, Wienecke A, Schmidt B, Antranikian GAppl Environ Microbiol10.1128/aem.57.5.1554-1559.19911991Amino Acid Sequence, Base Sequence, Binding Sites, Calcium/metabolism, Clostridium/enzymology/*genetics, Consensus Sequence, *Genes, Bacterial, Molecular Sequence Data, Molecular Weight, *Protein Processing, Post-Translational, RNA, Messenger/metabolism, Sequence Homology, Nucleic Acid, alpha-Amylases/*geneticsMetabolism
Enzymology32922127Simultaneous production of alpha and beta amylase enzymes using separate gene bearing recombinant vectors in the same Escherichia coli cells.Ozcan D, SIpahIoGlu HMTurk J Biol10.3906/biy-2001-712020Biotechnology

Reference

@idauthorscataloguedoi/urltitle
998Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2229)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2229
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87038Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47552.1StrainInfo: A central database for resolving microbial strain identifiers