Strain identifier
BacDive ID: 18049
Type strain:
Species: Thermoanaerobacterium thermosulfurigenes
Strain Designation: 4B
Strain history: <- J. G. Zeikus, University of Wisconsin, Department of Bacteriology, Madison, Wisconsin, USA; 4B (Clostridium thermosulfurigenes)
NCBI tax ID(s): 33950 (species)
General
@ref: 998
BacDive-ID: 18049
DSM-Number: 2229
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, alcohol production
description: Thermoanaerobacterium thermosulfurigenes 4B is an anaerobe, thermophilic bacterium that produces alcohol and was isolated from thermal spring.
NCBI tax id
- NCBI tax id: 33950
- Matching level: species
strain history
- @ref: 998
- history: <- J. G. Zeikus, University of Wisconsin, Department of Bacteriology, Madison, Wisconsin, USA; 4B (Clostridium thermosulfurigenes)
doi: 10.13145/bacdive18049.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Thermoanaerobacterium
- species: Thermoanaerobacterium thermosulfurigenes
- full scientific name: Thermoanaerobacterium thermosulfurigenes (Schink and Zeikus 1983) Lee et al. 1993
synonyms
@ref synonym 20215 Clostridium thermosulfurogenes 20215 Clostridium thermosulfurigenes
@ref: 998
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Thermoanaerobacterium
species: Thermoanaerobacterium thermosulfurigenes
full scientific name: Thermoanaerobacterium thermosulfurigenes (Schink and Zeikus 1983) Lee et al. 1993
strain designation: 4B
type strain: yes
Culture and growth conditions
culture medium
- @ref: 998
- name: THERMOANAEROBACTERIUM MEDIUM (DSMZ Medium 211)
- growth: yes
- link: https://mediadive.dsmz.de/medium/211
- composition: Name: THERMOANAEROBACTERIUM MEDIUM (DSMZ Medium 211) Composition: Na2HPO4 x 12 H2O 5.21141 g/l D-Glucose 4.91642 g/l NH4Cl 0.983284 g/l Yeast extract 0.983284 g/l Na2S x 9 H2O 0.491642 g/l KH2PO4 0.294985 g/l MgCl2 x 6 H2O 0.196657 g/l Nitrilotriacetic acid 0.12586 g/l NaCl 0.00983284 g/l FeCl2 x 4 H2O 0.00196657 g/l CoCl2 x 6 H2O 0.00167158 g/l FeSO4 x 7 H2O 0.00147493 g/l MnCl2 x 4 H2O 0.000983284 g/l ZnCl2 0.000983284 g/l CaCl2 x 2 H2O 0.000983284 g/l Sodium resazurin 0.000491642 g/l Na2SeO3 x 5 H2O 0.000294985 g/l NiCl2 x 6 H2O 0.000294985 g/l CuCl2 0.000196657 g/l Na2MoO4 x 2 H2O 9.83284e-05 g/l H3BO3 9.83284e-05 g/l Pyridoxine hydrochloride 4.91642e-05 g/l Calcium D-(+)-pantothenate 2.45821e-05 g/l p-Aminobenzoic acid 2.45821e-05 g/l (DL)-alpha-Lipoic acid 2.45821e-05 g/l Nicotinic acid 2.45821e-05 g/l Riboflavin 2.45821e-05 g/l Thiamine HCl 2.45821e-05 g/l Folic acid 9.83284e-06 g/l Biotin 9.83284e-06 g/l Vitamin B12 4.91642e-07 g/l Distilled water
culture temp
- @ref: 998
- growth: positive
- type: growth
- temperature: 60
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 998
- oxygen tolerance: anaerobe
compound production
@ref | compound |
---|---|
998 | sulfur from thiosulfate |
998 | pectin methylesterase |
998 | polygalacturonate hydrolase |
998 | ethanol |
998 | ß amylase, thermostable |
Isolation, sampling and environmental information
isolation
- @ref: 998
- sample type: thermal spring
- geographic location: Wyoming, Yellowstone Natl. Park, Octopus Spring
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_1467.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15961;96_838;97_980;98_1157;99_1467&stattab=map
- Last taxonomy: Thermoanaerobacterium xylanolyticum
- 16S sequence: L09171
- Sequence Identity:
- Total samples: 1780
- soil counts: 45
- aquatic counts: 83
- animal counts: 1638
- plant counts: 14
Safety information
risk assessment
- @ref: 998
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 998
- description: Thermoanaerobacterium thermosulfurigenes strain 4BT 16S ribosomal RNA gene, partial sequence
- accession: L09171
- length: 1574
- database: ena
- NCBI tax ID: 33950
Genome sequences
- @ref: 66792
- description: Thermoanaerobacterium thermosulfurigenes DSM 2229
- accession: 2861644854
- assembly level: draft
- database: img
- NCBI tax ID: 33950
GC content
- @ref: 998
- GC-content: 32.6
External links
@ref: 998
culture collection no.: DSM 2229, ATCC 33743
straininfo link
- @ref: 87038
- straininfo: 47552
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Enzymology | 1854207 | alpha-Amylase of Clostridium thermosulfurogenes EM1: nucleotide sequence of the gene, processing of the enzyme, and comparison of other alpha-amylases. | Bahl H, Burchhardt G, Spreinat A, Haeckel K, Wienecke A, Schmidt B, Antranikian G | Appl Environ Microbiol | 10.1128/aem.57.5.1554-1559.1991 | 1991 | Amino Acid Sequence, Base Sequence, Binding Sites, Calcium/metabolism, Clostridium/enzymology/*genetics, Consensus Sequence, *Genes, Bacterial, Molecular Sequence Data, Molecular Weight, *Protein Processing, Post-Translational, RNA, Messenger/metabolism, Sequence Homology, Nucleic Acid, alpha-Amylases/*genetics | Metabolism |
Enzymology | 32922127 | Simultaneous production of alpha and beta amylase enzymes using separate gene bearing recombinant vectors in the same Escherichia coli cells. | Ozcan D, SIpahIoGlu HM | Turk J Biol | 10.3906/biy-2001-71 | 2020 | Biotechnology |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
998 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2229) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2229 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
87038 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47552.1 | StrainInfo: A central database for resolving microbial strain identifiers |