Strain identifier
BacDive ID: 18037
Type strain: 
Species: Sulfobacillus thermotolerans
Strain Designation: Kr1
Strain history: <- G. I. Karavaiko; Kr1 <- V. S. Melamud and T. I. Bogdanova
NCBI tax ID(s): 338644 (species)
version 8.1 (current version)
General
@ref: 6832
BacDive-ID: 18037
DSM-Number: 17362
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive, rod-shaped
description: Sulfobacillus thermotolerans Kr1 is an aerobe, spore-forming, thermophilic bacterium that was isolated from sulphide gold-containing concentrat.
NCBI tax id
- NCBI tax id: 338644
- Matching level: species
strain history
- @ref: 6832
- history: <- G. I. Karavaiko; Kr1 <- V. S. Melamud and T. I. Bogdanova
doi: 10.13145/bacdive18037.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Sulfobacillaceae
- genus: Sulfobacillus
- species: Sulfobacillus thermotolerans
- full scientific name: Sulfobacillus thermotolerans Bogdanova et al. 2006
@ref: 6832
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Clostridiales
family: Alicyclobacillaceae
genus: Sulfobacillus
species: Sulfobacillus thermotolerans
full scientific name: Sulfobacillus thermotolerans Bogdanova et al. 2006
strain designation: Kr1
type strain: yes
Morphology
cell morphology
- @ref: 31715
- gram stain: positive
- cell length: 1.5-4.5 µm
- cell width: 0.8-1.2 µm
- cell shape: rod-shaped
- motility: no
pigmentation
- @ref: 31715
- production: yes
Culture and growth conditions
culture medium
- @ref: 6832
- name: SULFOBACILLUS MEDIUM (DSMZ Medium 665)
- growth: yes
- link: https://mediadive.dsmz.de/medium/665
- composition: Name: SULFOBACILLUS MEDIUM (DSMZ Medium 665) Composition: FeSO4 x 7 H2O 43.2909 g/l (NH4)2SO4 2.93829 g/l MgSO4 x 7 H2O 0.489716 g/l K2HPO4 0.489716 g/l KCl 0.0979431 g/l Ca(NO3)2 0.00979431 g/l Yeast extract 0.00391773 g/l H2SO4 Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6832 | positive | growth | 40 | thermophilic |
| 31715 | positive | growth | 20-60 | |
| 31715 | positive | optimum | 40 | thermophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 31715 | positive | growth | 1.2-2.4 | acidophile |
| 31715 | positive | optimum | 2 |
Physiology and metabolism
oxygen tolerance
- @ref: 31715
- oxygen tolerance: aerobe
spore formation
- @ref: 31715
- spore formation: yes
observation
- @ref: 31715
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 31715 | 28757 | fructose | + | carbon source |
| 31715 | 17234 | glucose | + | carbon source |
| 31715 | 25115 | malate | + | carbon source |
| 31715 | 17992 | sucrose | + | carbon source |
Isolation, sampling and environmental information
isolation
- @ref: 6832
- sample type: sulphide gold-containing concentrat
- geographic location: Eastern Siberia
- country: Russia
- origin.country: RUS
- continent: Asia
isolation source categories
- Cat1: #Condition
- Cat2: #Sulfuric
taxonmaps
- @ref: 69479
- File name: preview.99_61564.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15916;96_9389;97_11290;98_17404;99_61564&stattab=map
- Last taxonomy: Sulfobacillus thermotolerans subclade
- 16S sequence: DQ124681
- Sequence Identity:
- Total samples: 89
- soil counts: 15
- aquatic counts: 72
- animal counts: 2
Safety information
risk assessment
- @ref: 6832
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6832
- description: Sulfobacillus thermotolerans strain Kr1 16S ribosomal RNA gene, partial sequence
- accession: DQ124681
- length: 1433
- database: ena
- NCBI tax ID: 338644
Genome sequences
- @ref: 66792
- description: Sulfobacillus thermotolerans Kr1
- accession: GCA_002951815
- assembly level: complete
- database: ncbi
- NCBI tax ID: 338644
GC content
- @ref: 6832
- GC-content: 48.2
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| flagellated | no | 88.271 | no |
| gram-positive | yes | 77.123 | yes |
| anaerobic | no | 94.973 | yes |
| aerobic | yes | 58.361 | yes |
| halophile | no | 95.626 | no |
| spore-forming | yes | 82.434 | yes |
| glucose-util | yes | 80.233 | no |
| thermophile | yes | 98.179 | no |
| motile | yes | 72.092 | no |
| glucose-ferment | no | 84.406 | no |
External links
@ref: 6832
culture collection no.: DSM 17362, VKM B-2339
straininfo link
- @ref: 87026
- straininfo: 291013
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 16627651 | Sulfobacillus thermotolerans sp. nov., a thermotolerant, chemolithotrophic bacterium. | Bogdanova TI, Tsaplina IA, Kondrat'eva TF, Duda VI, Suzina NE, Melamud VS, Tourova TP, Karavaiko GI | Int J Syst Evol Microbiol | 10.1099/ijs.0.64106-0 | 2006 | Base Composition, Carbohydrate Metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Environmental Microbiology, Ferrous Compounds/metabolism, Genes, rRNA, Glutamic Acid/metabolism, Glutathione/metabolism, Gold, Gram-Positive Endospore-Forming Rods/*classification/cytology/*isolation & purification/physiology, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfides/metabolism, Sulfur/metabolism, Temperature, Tetrathionic Acid/metabolism | Metabolism |
| Phylogeny | 34236956 | Sulfobacillus harzensis sp. nov., an acidophilic bacterium inhabiting mine tailings from a polymetallic mine. | Zhang R, Hedrich S, Jin D, Breuker A, Schippers A | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004871 | 2021 | Bacterial Typing Techniques, Base Composition, Clostridiales/*classification/isolation & purification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Germany, *Mining, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 6832 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17362) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17362 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 31715 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27991 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 87026 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID291013.1 | StrainInfo: A central database for resolving microbial strain identifiers |