Strain identifier

BacDive ID: 18027

Type strain: Yes

Species: Caldicellulosiruptor owensensis

Strain Designation: OL

Strain history: <- ATCC <- C.-Y. Huang, University of California, School of Public Health, Los Angeles, USA; OL

NCBI tax ID(s): 632518 (strain), 55205 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 4904

BacDive-ID: 18027

DSM-Number: 13100

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, chemoorganotroph, thermophilic, colony-forming

description: Caldicellulosiruptor owensensis OL is an anaerobe, chemoorganotroph, thermophilic bacterium that forms circular colonies and was isolated from freshwater sediment.

NCBI tax id

NCBI tax idMatching level
55205species
632518strain

strain history

  • @ref: 4904
  • history: <- ATCC <- C.-Y. Huang, University of California, School of Public Health, Los Angeles, USA; OL

doi: 10.13145/bacdive18027.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Syntrophomonadaceae
  • genus: Caldicellulosiruptor
  • species: Caldicellulosiruptor owensensis
  • full scientific name: Caldicellulosiruptor owensensis Huang et al. 1998

@ref: 4904

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridia, not assigned to family

genus: Caldicellulosiruptor

species: Caldicellulosiruptor owensensis

full scientific name: Caldicellulosiruptor owensensis Huang et al. 1998

strain designation: OL

type strain: yes

Morphology

colony morphology

  • @ref: 43370
  • colony size: 1-2 mm
  • colony color: yellowish opaque
  • colony shape: circular
  • medium used: CBM medium

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4904CALDICELLULOSIRUPTOR OWENSIS MEDIUM (DSMZ Medium 144b)yeshttps://mediadive.dsmz.de/medium/144bName: CALDICELLULOSIRUPTOR OWENSIS MEDIUM (DSMZ Medium 144b) Composition: Trypticase peptone 10.0 g/l D-Glucose 5.0 g/l Yeast extract 3.0 g/l Na2CO3 2.0 g/l Na2S x 9 H2O 1.0 g/l NaCl 0.9 g/l NH4Cl 0.9 g/l K2HPO4 0.4 g/l MgCl2 x 6 H2O 0.1 g/l CaCl2 x 2 H2O 0.05 g/l Na2-EDTA x 2 H2O 0.005 g/l CoCl2 x 6 H2O 0.0015 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l ZnCl2 0.001 g/l Sodium resazurin 0.0005 g/l AlCl3 x 6 H2O 0.0004 g/l Na2WO4 x 2 H2O 0.0004 g/l Na2SeO3 x 5 H2O 0.0003 g/l NiCl2 x 6 H2O 0.0002 g/l CuCl2 x 2 H2O 0.0002 g/l Na2MoO4 x 2 H2O 0.0001 g/l H3BO3 0.0001 g/l Pyridoxine hydrochloride 5e-05 g/l Riboflavin 2.5e-05 g/l (DL)-alpha-Lipoic acid 2.5e-05 g/l p-Aminobenzoic acid 2.5e-05 g/l Calcium D-(+)-pantothenate 2.5e-05 g/l Thiamine HCl 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l Biotin 1e-05 g/l Folic acid 1e-05 g/l Vitamin B12 5e-07 g/l Distilled water
43370CBM mediumyes1g/L Yeast extract, 5g/L xylan, 1g/L NH4Cl, 0.1g/L NaCl, 0.1 g/L MgCl*6 H2O, 0.05g/L CaCl2*2 H2O, 0.4 g/L K2HPO4, 0.0005g/L resazurin, 1mL/L vitamin solution and 10 mL/l trace mineral solution. 3g NaHCO3 was added wafter boiling. The vitamin solution consists of 2 mg/L biotin, 2mg/L folic acid, 10 mg/L pyridoxine hydrochloride, 5 mg/L thiamine hydrochloride, 5 mg/L riboflavine, 5 mg/L nicotinic acid, 5 mg/L DL- calcium panthothenate, 0.1 mg/L Vitamin B12, 5 mg/L p-amino-benzoic acid and 5 mg/L lipoic acid. the trace mineral solution consisted of 0.01g/L H2SeO3, 0.01g MnCl2*4 H2O, 0.1g/L FeSO4*7 H2O, 0.15g/L CoCl2*6 H2O, 0.1g/L ZnCl2, 0.01g/L H3BO3, 0.01g/L Na2MoO4*2 H2O, 0.02g/L CuCl2*2 H2O, 0.02g/L NiSO4*6 H2O, 0.04g/L AlCl3*6 H2O, 0.03g/L NaWO4, and 0.5g/L disodium EDTA.

culture temp

@refgrowthtypetemperaturerange
4904positivegrowth70thermophilic
43370positiveoptimum75thermophilic
43370positivegrowth50-80hyperthermophilic

culture pH

@refabilitytypepHPH range
43370positivegrowth5.5-9alkaliphile
43370positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4904anaerobe
43370obligate anaerobe

nutrition type

  • @ref: 43370
  • type: chemoorganotroph

spore formation

  • @ref: 43370
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4337030089acetate-growth
4337027613amygdalin-growth
4337022599arabinose+growth
4337018305arbutin-growth
4337017057cellobiose+growth
4337062968cellulose+degradation
4337062968cellulose+growth
4337023652dextrin+growth
4337017113erythritol-growth
4337028757fructose+growth
4337028260galactose+growth
4337017234glucose+growth
4337017754glycerol-growth
4337028087glycogen+growth
4337017268myo-inositol+growth
4337024996lactate-growth
4337017716lactose+growth
4337017306maltose+growth
4337029864mannitol+growth
4337037684mannose+growth
433706731melezitose-growth
4337028053melibiose-growth
4337017790methanol-growth
4337017632nitrate-reduction
4337017309pectin+growth
43370peptone-growth
4337015361pyruvate-growth
4337016634raffinose+growth
4337026546rhamnose+growth
4337033942ribose+growth
4337030911sorbitol-growth
4337028017starch+growth
4337017992sucrose+growth
4337033954tagatose+growth
4337027082trehalose-growth
4337037166xylan+growth
4337018222xylose+growth
43370yeast extract (0.01 %, w/v)+growth

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4337028971ampicillinyesyes100 µg/mL
4337017698chloramphenicolyesyes100 µg/mL
4337018208penicillin gyesyes100 µg/mL
4337017076streptomycinyesyes100 µg/mL
4337048923erythromycinyesyes200 µg/mL
4337027902tetracyclineyesyes100 µg/mL

metabolite production

@refChebi-IDmetaboliteproduction
4337030089acetateyes
4337016526carbon dioxideyes
4337018276dihydrogenyes
4337016236ethanolyes
4337024996lactateyes
4337033403elemental sulfurno

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4904freshwater sedimentCA, Owens LakeUSAUSANorth America
43370sediments from Owens LakeCaliforniaUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Aquatic#Freshwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_1174.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_153;96_509;97_797;98_924;99_1174&stattab=map
  • Last taxonomy: Caldicellulosiruptor owensensis subclade
  • 16S sequence: U80596
  • Sequence Identity:
  • Total samples: 21
  • aquatic counts: 11
  • animal counts: 10

Safety information

risk assessment

  • @ref: 4904
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4904
  • description: Caldicellulosiruptor owensense 16S ribosomal RNA gene, partial sequence
  • accession: U80596
  • length: 1539
  • database: ena
  • NCBI tax ID: 632518

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Caldicellulosiruptor owensensis OLGCA_000166335completencbi632518
66792Caldicellulosiruptor owensensis OL632518.3completepatric632518
66792Caldicellulosiruptor owensensis OL649633025completeimg632518

GC content

  • @ref: 4904
  • GC-content: 36.6
  • method: Buoyant density centrifugation (BD)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes82.327no
flagellatedno87.907no
gram-positiveyes83.766no
anaerobicyes99.422yes
aerobicno98.678no
halophileno96.572no
spore-formingno74.145yes
thermophileyes100yes
glucose-utilyes90.652yes
glucose-fermentyes52.132no

External links

@ref: 4904

culture collection no.: DSM 13100, ATCC 700167

straininfo link

  • @ref: 87016
  • straininfo: 43803

literature

  • topic: Phylogeny
  • Pubmed-ID: 9542080
  • title: Caldicellulosiruptor owensensis sp. nov., an anaerobic, extremely thermophilic, xylanolytic bacterium.
  • authors: Huang CY, Patel BK, Mah RA, Baresi L
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-48-1-91
  • year: 1998
  • mesh: Bacteria, Anaerobic/*classification/genetics/*metabolism, Bacteriological Techniques, DNA, Bacterial/analysis, Flagella/ultrastructure, Hot Temperature, Microscopy, Electron, Molecular Sequence Data, RNA, Bacterial/analysis, RNA, Ribosomal, 16S/analysis, *Soil Microbiology, Xylans/*metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4904Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13100)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13100
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
43370Chi-Yu Huang, Bharat K. Patel, Robert A. Mah, Larry Baresi10.1099/00207713-48-1-91Caldicellulosiruptor owensensis sp. nov., an anaerobic, extremely thermophilic, xylanolytic bacteriumIJSEM 48: 91-97 19989542080
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87016Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43803.1StrainInfo: A central database for resolving microbial strain identifiers