Strain identifier

BacDive ID: 18023

Type strain: Yes

Species: Caldicellulosiruptor acetigenus

Strain Designation: X6B

Strain history: <- B. Ahring, X6B

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2957

BacDive-ID: 18023

DSM-Number: 7040

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-positive

description: Caldicellulosiruptor acetigenus X6B is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from hot spring.

NCBI tax id

NCBI tax idMatching level
1121259strain
301953species

strain history

  • @ref: 2957
  • history: <- B. Ahring, X6B

doi: 10.13145/bacdive18023.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Syntrophomonadaceae
  • genus: Caldicellulosiruptor
  • species: Caldicellulosiruptor acetigenus
  • full scientific name: Caldicellulosiruptor acetigenus (Nielsen et al. 1994) Onyenwoke et al. 2006
  • synonyms

    @refsynonym
    20215Caldicellulosiruptor kristjanssonii
    20215Caldicellulosiruptor lactoaceticus
    20215Thermoanaerobium acetigenum

@ref: 2957

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridia, not assigned to family

genus: Caldicellulosiruptor

species: Caldicellulosiruptor acetigenus

full scientific name: Caldicellulosiruptor acetigenus (Nielsen et al. 1994) Onyenwoke et al. 2006

strain designation: X6B

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 99.999

Culture and growth conditions

culture medium

  • @ref: 2957
  • name: CALDICELLULOSIRUPTOR ACETIGENUS MEDIUM (DSMZ Medium 144a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/144a
  • composition: Name: CALDICELLULOSIRUPTOR ACETIGENUS MEDIUM (DSMZ Medium 144a) Composition: D-Glucose 5.0 g/l K2HPO4 1.5 g/l Na2S x 9 H2O 1.0 g/l Yeast extract 1.0 g/l NH4Cl 0.9 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l Nitrilotriacetic acid 0.1152 g/l NaCl 0.009 g/l FeSO4 x 7 H2O 0.003 g/l FeCl2 x 4 H2O 0.0018 g/l CoCl2 x 6 H2O 0.00153 g/l MnCl2 x 4 H2O 0.0009 g/l CaCl2 x 2 H2O 0.0009 g/l ZnCl2 0.0009 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.00027 g/l Na2SeO3 x 5 H2O 0.00027 g/l CuCl2 0.00018 g/l H3BO3 9e-05 g/l Na2MoO4 x 2 H2O 9e-05 g/l Pyridoxine hydrochloride 5e-05 g/l p-Aminobenzoic acid 2.5e-05 g/l (DL)-alpha-Lipoic acid 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l Riboflavin 2.5e-05 g/l Thiamine HCl 2.5e-05 g/l Calcium D-(+)-pantothenate 2.5e-05 g/l Biotin 1e-05 g/l Folic acid 1e-05 g/l Vitamin B12 5e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerangeconfidence
2957positivegrowth70thermophilic
69480thermophilic100

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
2957anaerobe
69480anaerobe99.999

Isolation, sampling and environmental information

isolation

  • @ref: 2957
  • sample type: hot spring
  • country: Iceland
  • origin.country: ISL
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

  • @ref: 2957
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
2957Caldicellulosiruptor acetigenus clone 2 16S ribosomal RNA gene, partial sequenceAY7724771413ena301953
2957Caldicellulosiruptor acetigenus clone 1 16S ribosomal RNA gene, partial sequenceAY7724761421ena301953

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Caldicellulosiruptor acetigenus DSM 7040GCA_000421725contigncbi1121259
66792Caldicellulosiruptor acetigenus DSM 70401121259.3wgspatric1121259
66792Caldicellulosiruptor acetigenus DSM 70402524614853draftimg1121259

GC content

  • @ref: 2957
  • GC-content: 35.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes68no
motileyes84.79no
flagellatedno83.989no
gram-positiveyes81.938no
anaerobicyes99.209no
aerobicno98.547yes
halophileno98.117no
spore-formingno59.694no
thermophileyes100no
glucose-utilyes89.508no
glucose-fermentno55.743no

External links

@ref: 2957

culture collection no.: DSM 7040, ATCC BAA 1149

straininfo link

  • @ref: 87012
  • straininfo: 48349

literature

  • topic: Phylogeny
  • Pubmed-ID: 16738119
  • title: Reclassification of Thermoanaerobium acetigenum as Caldicellulosiruptor acetigenus comb. nov. and emendation of the genus description.
  • authors: Onyenwoke RU, Lee YJ, Dabrowski S, Ahring BK, Wiegel J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63723-0
  • year: 2006
  • mesh: Clostridium/*classification/genetics/growth & development, Molecular Sequence Data, *Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Reproducibility of Results, Terminology as Topic, Thermoanaerobacterium/*classification/genetics/growth & development
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
2957Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7040)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7040
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
87012Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48349.1StrainInfo: A central database for resolving microbial strain identifiers