Strain identifier

BacDive ID: 180

Type strain: Yes

Species: Schaalia canis

Strain Designation: IFR M2289/98/2, M2289/98/2

Strain history: CIP <- 1999, CCUG <- MD Collins, IFR, Reading, UK: strain M2289/98/2 <- L. Hoyles, IFR, Reading, UK <- G. Foster, Inverness, UK

NCBI tax ID(s): 100469 (species)

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General

@ref: 5985

BacDive-ID: 180

DSM-Number: 15536

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped, animal pathogen

description: Schaalia canis IFR M2289/98/2 is a microaerophile, mesophilic, Gram-positive animal pathogen that was isolated from dog vagina.

NCBI tax id

  • NCBI tax id: 100469
  • Matching level: species

strain history

@refhistory
5985<- CCUG <- M. D. Collins <- G. Foster
117142CIP <- 1999, CCUG <- MD Collins, IFR, Reading, UK: strain M2289/98/2 <- L. Hoyles, IFR, Reading, UK <- G. Foster, Inverness, UK

doi: 10.13145/bacdive180.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Schaalia
  • species: Schaalia canis
  • full scientific name: Schaalia canis (Hoyles et al. 2000) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces canis

@ref: 5985

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Schaalia

species: Schaalia canis

full scientific name: Schaalia canis (Hoyles et al. 2000) Nouioui et al. 2018

strain designation: IFR M2289/98/2, M2289/98/2

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
23115positiverod-shapedno
117142positiverod-shapedno

colony morphology

  • @ref: 117142

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5985FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)yeshttps://mediadive.dsmz.de/medium/1203Name: FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203) Composition: Horse blood 100.0 g/l Fastidious Anaerobe Agar 45.7 g/l Distilled water
5985COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
39501MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
117142CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5985positivegrowth37mesophilic
39501positivegrowth37mesophilic
117142positivegrowth30-45
117142nogrowth10psychrophilic
117142nogrowth25mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5985microaerophile
23115facultative anaerobe
117142facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
117142NaClpositivegrowth0-2 %
117142NaClnogrowth4 %
117142NaClnogrowth6 %
117142NaClnogrowth8 %
117142NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2311523456cyclodextrin-builds acid from
2311518333D-arabitol-builds acid from
2311530849L-arabinose-builds acid from
2311529864mannitol-builds acid from
231156731melezitose-builds acid from
2311528053melibiose-builds acid from
23115320055methyl beta-D-glucopyranoside-builds acid from
2311530911sorbitol-builds acid from
2311533954tagatose-builds acid from
2311527082trehalose-builds acid from
2311518403L-arabitol-fermentation
2311529864mannitol-fermentation
231156731melezitose-fermentation
2311537657methyl D-glucoside-fermentation
2311527082trehalose-fermentation
231154853esculin-hydrolysis
231155291gelatin-hydrolysis
23115606565hippurate-hydrolysis
2311515688acetoin-other
2311517632nitrate-reduction
2311517634D-glucose+builds acid from
2311516988D-ribose+builds acid from
2311565327D-xylose+builds acid from
2311528087glycogen+builds acid from
2311517716lactose+builds acid from
2311517306maltose+builds acid from
2311527941pullulan+builds acid from
2311565327D-xylose+fermentation
2311517234glucose+fermentation
2311528087glycogen+fermentation
2311530849L-arabinose+fermentation
2311527941pullulan+fermentation
2311533942ribose+fermentation
2311516634raffinose+/-builds acid from
2311517992sucrose+/-builds acid from
2311517992sucrose+/-fermentation
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol+builds acid from
6837118333D-arabitol+builds acid from
6837118287L-fucose+builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose+builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose+builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose+builds acid from
6837117108D-arabinose+builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
1171424853esculin-hydrolysis
117142606565hippurate+hydrolysis
11714217632nitrate-reduction
11714216301nitrite-reduction
11714217632nitrate+respiration

antibiotic resistance

  • @ref: 117142
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 117142
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11714215688acetoin-
11714217234glucose+

enzymes

@refvalueactivityec
23115acid phosphatase-3.1.3.2
23115alkaline phosphatase-3.1.3.1
23115alpha-fucosidase+3.2.1.51
23115alpha-galactosidase+3.2.1.22
23115alpha-glucosidase+3.2.1.20
23115alpha-mannosidase-3.2.1.24
23115arginine dihydrolase-3.5.3.6
23115beta-galactosidase+3.2.1.23
23115beta-glucosidase-3.2.1.21
23115beta-glucuronidase-3.2.1.31
23115beta-mannosidase-3.2.1.25
23115catalase+1.11.1.6
23115chymotrypsin-3.4.4.5
23115cystine arylamidase+3.4.11.3
23115esterase (C 4)-
23115esterase lipase (C 8)+
23115glycyl tryptophan arylamidase+/-
23115leucine arylamidase+3.4.11.1
23115lipase (C 14)-
23115N-acetyl-beta-glucosaminidase+3.2.1.52
23115phosphoamidase-3.9.1.1
23115pyrazinamidase+3.5.1.B15
23115pyroglutamic acid arylamidase-
23115pyrrolidonyl arylamidase-3.4.19.3
23115tripeptide aminopeptidase+3.4.11.4
23115trypsin-3.4.21.4
23115urease-3.5.1.5
23115valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
117142oxidase-
117142beta-galactosidase+3.2.1.23
117142alcohol dehydrogenase-1.1.1.1
117142gelatinase-
117142amylase+
117142DNase-
117142caseinase-3.4.21.50
117142catalase+1.11.1.6
117142tween esterase+
117142lecithinase+
117142lipase+
117142lysine decarboxylase+4.1.1.18
117142ornithine decarboxylase-4.1.1.17
117142phenylalanine ammonia-lyase-4.3.1.24
117142protease+
117142tryptophan deaminase-
117142urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117142--++-+-------+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117142+-++++---++++---+---++----+++++---+++--++--++++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
5985dog vaginaInvernessUnited KingdomGBREurope
55183Dog vagina,history of infertility,polymicrobialInvernessUnited KingdomGBREurope1998
117142Animal, Dog, vagina

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body-Site#Urogenital tract#Vagina

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
5985yes2Risk group (German classification)
1171422Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5985
  • description: Actinomyces sp. CCUG 41706 mRNA for 16S ribosomal RNA (16S rRNA gene)
  • accession: AJ243891
  • length: 1428
  • database: ena
  • NCBI tax ID: 100469

External links

@ref: 5985

culture collection no.: DSM 15536, CCUG 41706, CIP 106351

straininfo link

  • @ref: 69862
  • straininfo: 87794

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10939662Actinomyces canis sp. nov., isolated from dogs.Hoyles L, Falsen E, Foster G, Pascual C, Greko C, Collins MDInt J Syst Evol Microbiol10.1099/00207713-50-4-15472000Actinomyces/*classification/isolation & purification/physiology, Actinomycosis/*veterinary, Animals, Catalase, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Dog Diseases/*microbiology, Dogs, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysisGenetics
Phylogeny35460225Description of Acinetobacter ihumii sp. nov., Microbacterium ihumii sp. nov., and Gulosibacter massiliensis sp. nov., three new bacteria isolated from human blood.Yacouba A, Sissoko S, Tchoupou Saha OF, Haddad G, Dubourg G, Gouriet F, Tidjani Alou M, Alibar S, Million M, Lagier JC, Raoult D, Fenollar F, Fournier PE, Lo CIFEMS Microbiol Lett10.1093/femsle/fnac0382022*Acinetobacter/genetics, *Actinomycetales/genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids, Humans, Microbacterium, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5985Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15536)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15536
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23115L Hoyles, E Falsen, G Foster, C Pascual, C Greko, M D Collins10.1099/00207713-50-4-1547Actinomyces canis sp. nov., isolated from dogs.IJSEM 50: 1547-1551 200010939662
39501Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18472
55183Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 41706)https://www.ccug.se/strain?id=41706
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69862Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID87794.1StrainInfo: A central database for resolving microbial strain identifiers
117142Curators of the CIPCollection of Institut Pasteur (CIP 106351)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106351