Strain identifier

BacDive ID: 17969

Type strain: Yes

Species: Anaerococcus tetradius

Strain history: CIP <- 1993, ATCC <- T. Ezaki: strain GIFU 7672

NCBI tax ID(s): 525255 (strain), 33036 (species)

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General

@ref: 1259

BacDive-ID: 17969

DSM-Number: 2951

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, human pathogen

description: Anaerococcus tetradius DSM 2951 is an anaerobe, mesophilic human pathogen that was isolated from human vagina.

NCBI tax id

NCBI tax idMatching level
525255strain
33036species

strain history

@refhistory
1259<- ATCC <- T. Ezaki, GIFU 7672
67770T. Ezaki GIFU 7672.
123489CIP <- 1993, ATCC <- T. Ezaki: strain GIFU 7672

doi: 10.13145/bacdive17969.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptoniphilaceae
  • genus: Anaerococcus
  • species: Anaerococcus tetradius
  • full scientific name: Anaerococcus tetradius (Ezaki et al. 1983 ex Choukévitch 1911) Ezaki et al. 2001

  • synonyms

    • @ref: 20215
    • synonym: Peptostreptococcus tetradius

@ref: 1259

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Peptoniphilaceae

genus: Anaerococcus

species: Anaerococcus tetradius

full scientific name: Anaerococcus tetradius (Ezaki et al. 1983) Ezaki et al. 2001

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.331
69480100positive
123489nopositivecoccus-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1259PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
1259CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
40869MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123489CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20
123489CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
1259positivegrowth37mesophilic
40869positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
1259anaerobe
69480anaerobe98.846
123489anaerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.996

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12348917632nitrate-reduction
12348916301nitrite-reduction
6838016199urea-hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
12348935581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase+3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380urease-3.5.1.5
123489urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123489--++------++--++----

API rID32A

@refUREADH Argbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
1259-+/---+--+-+------+/------+/---+/---

Isolation, sampling and environmental information

isolation

@refsample typeisolation date
1259human vagina
67770Vaginal discharge
123489Human, Vagina1979

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Urogenital tract#Vagina
#Host#Human#Female

taxonmaps

  • @ref: 69479
  • File name: preview.99_5609.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_172;96_952;97_1110;98_4185;99_5609&stattab=map
  • Last taxonomy: Anaerococcus tetradius subclade
  • 16S sequence: LC036320
  • Sequence Identity:
  • Total samples: 8636
  • soil counts: 86
  • aquatic counts: 44
  • animal counts: 8492
  • plant counts: 14

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
1259yes, in single cases1Risk group (German classification)
1234891Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
1259Anaerococcus tetradius gene for 16S ribosomal RNA, partial sequence, isolate: GIFU 7672D141421377ena33036
67770Anaerococcus tetradius gene for 16S ribosomal RNA, partial sequence, strain: JCM 1964LC0363201487ena33036

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerococcus tetradius ATCC 35098525255.3wgspatric525255
66792Anaerococcus tetradius ATCC 35098643886055draftimg525255
67770Anaerococcus tetradius ATCC 35098GCA_000159095scaffoldncbi525255

GC content

@refGC-content
125930.0
6777030

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
gram-positiveyes95.845no
anaerobicyes99.443yes
halophileno59.13no
spore-formingno92.885no
glucose-utilyes88.117no
aerobicno98.13no
motileno94.548no
flagellatedno96.541no
thermophileno97.927no
glucose-fermentyes77.758no

External links

@ref: 1259

culture collection no.: DSM 2951, ATCC 35098, CCM 3634, JCM 1964, GIFU 7672, CCUG 17637, CIP 103927, LMG 14264

straininfo link

  • @ref: 86956
  • straininfo: 7938

literature

  • Pubmed-ID: 26781497
  • title: Further Identification of Novel Lantibiotic Operons Using LanM-Based Genome Mining.
  • authors: O'Sullivan O, Begley M, Ross RP, Cotter PD, Hill C
  • journal: Probiotics Antimicrob Proteins
  • DOI: 10.1007/s12602-011-9062-y
  • year: 2011

Reference

@idauthorscataloguedoi/urltitle
1259Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2951)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2951
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
40869Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15779
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86956Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7938.1StrainInfo: A central database for resolving microbial strain identifiers
123489Curators of the CIPCollection of Institut Pasteur (CIP 103927)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103927