Archive BacDiveID:10.13145/bacdive17964.20230509.8.1

Strain identifier

BacDive ID: 17964

Type strain:

Species: Anaerobranca californiensis

Strain Designation: PAOHA-1

Strain history: <- V. Gorlenko, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; PAOHA-1 <- V. Gorlenko {2000}

NCBI tax ID(s): 1120989 (strain), 182411 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 5563

BacDive-ID: 17964

DSM-Number: 14826

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-negative, motile, rod-shaped

description: Anaerobranca californiensis PAOHA-1 is an anaerobe, thermophilic, Gram-negative bacterium that was isolated from alkaline hot spring.

NCBI tax id

NCBI tax id	Matching level
1120989	strain
182411	species

strain history

@ref: 5563
history: <- V. Gorlenko, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; PAOHA-1 <- V. Gorlenko {2000}

doi: 10.13145/bacdive17964.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Clostridia
order: Eubacteriales
family: Syntrophomonadaceae
genus: Anaerobranca
species: Anaerobranca californiensis
full scientific name: Anaerobranca californiensis Gorlenko et al. 2004

@ref: 5563

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Proteinivoraceae

genus: Anaerobranca

species: Anaerobranca californiensis

full scientific name: Anaerobranca californiensis Gorlenko et al. 2004

strain designation: PAOHA-1

type strain: yes

Morphology

cell morphology

@ref	gram stain	cell length	cell width	cell shape	motility	confidence
30141	negative	3.7 µm	0.285 µm	rod-shaped	yes
69480	positive					99.998

Culture and growth conditions

culture medium

@ref: 5563
name: ANAEROBRANCA GOTTSCHALKII MEDIUM (DSMZ Medium 895)
growth: yes
link: https://mediadive.dsmz.de/medium/895
composition: Name: ANAEROBRANCA GOTTSCHALKII MEDIUM (DSMZ Medium 895; with strain-specific modifications) Composition: Na2S2O3 x 5 H2O 3.94867 g/l Na2CO3 2.9615 g/l Tryptone 1.97433 g/l (NH4)2SO4 0.987167 g/l L-Cysteine HCl x H2O 0.493583 g/l K2HPO4 0.493583 g/l NH4Cl 0.394867 g/l Yeast extract 0.246792 g/l MgSO4 x 7 H2O 0.029615 g/l Nitrilotriacetic acid 0.0148075 g/l NaCl 0.00987167 g/l MnSO4 x H2O 0.00493583 g/l CoSO4 x 7 H2O 0.0017769 g/l ZnSO4 x 7 H2O 0.0017769 g/l CaCl2 x 2 H2O 0.000987167 g/l FeSO4 x 7 H2O 0.000987167 g/l Sodium resazurin 0.000493583 g/l NiCl2 x 6 H2O 0.00029615 g/l AlK(SO4)2 x 12 H2O 0.000197433 g/l CuSO4 x 5 H2O 9.87167e-05 g/l H3BO3 9.87167e-05 g/l Pyridoxine hydrochloride 9.87167e-05 g/l Na2MoO4 x 2 H2O 9.87167e-05 g/l p-Aminobenzoic acid 4.93583e-05 g/l (DL)-alpha-Lipoic acid 4.93583e-05 g/l Calcium D-(+)-pantothenate 4.93583e-05 g/l Nicotinic acid 4.93583e-05 g/l Riboflavin 4.93583e-05 g/l Thiamine HCl 4.93583e-05 g/l Folic acid 1.97433e-05 g/l Biotin 1.97433e-05 g/l Na2WO4 x 2 H2O 3.94867e-06 g/l Na2SeO3 x 5 H2O 2.9615e-06 g/l Vitamin B12 9.87167e-07 g/l Distilled water

culture temp

@ref	growth	type	temperature	range	confidence
5563	positive	growth	60	thermophilic
30141	positive	growth	45-70	thermophilic
30141	positive	optimum	58	thermophilic
69480				thermophilic	96.997

culture pH

@ref	ability	type	pH	PH range
30141	positive	growth	8.6-10.4	alkaliphile
30141	positive	optimum	9.25

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance	confidence
5563	anaerobe
30141	anaerobe
69480	anaerobe	99.586

spore formation

@ref	spore formation	confidence
30141	no
69480	yes	96.079

halophily

@ref	salt	growth	tested relation	concentration
30141	NaCl	positive	growth	0-6 %
30141	NaCl	positive	optimum	1.75 %

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
30141	17057	cellobiose	+	carbon source
30141	28757	fructose	+	carbon source
30141	17754	glycerol	+	carbon source
30141	17306	maltose	+	carbon source
30141	15361	pyruvate	+	carbon source
30141	17992	sucrose	+	carbon source

metabolite production

@ref: 30141
Chebi-ID: 16136
metabolite: hydrogen sulfide
production: yes

Isolation, sampling and environmental information

isolation

@ref: 5563
sample type: alkaline hot spring
geographic location: California, Mono lake, Paoha island
country: USA
origin.country: USA
continent: North America

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)
#Condition	#Alkaline

taxonmaps

@ref: 69479
File name: preview.99_36557.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3665;96_17455;97_21478;98_26936;99_36557&stattab=map
Last taxonomy: Anaerobranca
16S sequence: AY064217
Sequence Identity:
Total samples: 4
soil counts: 1
animal counts: 3

Safety information

risk assessment

@ref: 5563
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 5563
description: Anaerobranca californiensis strain PAOHA-1 16S ribosomal RNA gene, partial sequence
accession: AY064217
length: 1489
database: ena
NCBI tax ID: 182411

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Anaerobranca californiensis DSM 14826	GCA_900142275	contig	ncbi	1120989
66792	Anaerobranca californiensis DSM 14826	1120989.3	wgs	patric	1120989
66792	Anaerobranca californiensis DSM 14826	2585428191	draft	img	1120989

GC content

@ref	GC-content
5563	30.3
30141	30

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	yes	53	no
motile	yes	77.765	no
flagellated	no	87.288	no
gram-positive	yes	61.81	no
anaerobic	yes	98.746	yes
aerobic	no	97.642	yes
halophile	no	55.722	no
spore-forming	yes	68.742	no
glucose-util	yes	88.96	no
thermophile	yes	99.998	yes
glucose-ferment	yes	52.849	no

External links

@ref: 5563

culture collection no.: DSM 14826, UNIQEM 227

straininfo link

@ref: 86951
straininfo: 132010

literature

topic: Phylogeny
Pubmed-ID: 15143017
title: Anaerobranca californiensis sp. nov., an anaerobic, alkalithermophilic, fermentative bacterium isolated from a hot spring on Mono Lake.
authors: Gorlenko V, Tsapin A, Namsaraev Z, Teal T, Tourova T, Engler D, Mielke R, Nealson K
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijs.0.02909-0
year: 2004
mesh: Bacteria, Anaerobic/*classification/genetics/*isolation & purification/metabolism, California, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fermentation, Fresh Water/microbiology, Hot Temperature, Hydrogen-Ion Concentration, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sulfides/metabolism
topic2: Metabolism

Reference

@id	authors	catalogue	doi/url	title	ID_cross_reference	pubmed	journal
5563	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14826)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-14826
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30141	Barberan A, Caceres Velazquez H, Jones S, Fierer N.		10.1128/mSphere.00237-17	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information	26492	28776041
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg		10.1093/nar/gkaa1025	BRENDA, the ELIXIR core data resource in 2021: new developments and updates		33211880	Nucleic Acids Res. 49: D498-D508 2020
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
86951	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID132010.1	StrainInfo: A central database for resolving microbial strain identifiers