Strain identifier

BacDive ID: 1793

Type strain: Yes

Species: Bosea robiniae

Strain Designation: R-46070

Strain history: <- LMG <- A. Willems and S. De Meyer, Group Biochemistry and Microbiology, University of Ghent

NCBI tax ID(s): 1036780 (species)

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General

@ref: 18269

BacDive-ID: 1793

DSM-Number: 26672

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Bosea robiniae R-46070 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodules of Robinia pseudoacacia.

NCBI tax id

  • NCBI tax id: 1036780
  • Matching level: species

strain history

  • @ref: 18269
  • history: <- LMG <- A. Willems and S. De Meyer, Group Biochemistry and Microbiology, University of Ghent

doi: 10.13145/bacdive1793.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Boseaceae
  • genus: Bosea
  • species: Bosea robiniae
  • full scientific name: Bosea robiniae De Meyer and Willems 2012

@ref: 18269

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Boseaceae

genus: Bosea

species: Bosea robiniae

full scientific name: Bosea robiniae De Meyer and Willems 2012

strain designation: R-46070

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23325negative1.1-1.4 µm0.8 µmrod-shapedyes
69480yes97.495
69480negative99.991

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
23325YMAyes
18269NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18269positivegrowth28mesophilic
23325positivegrowth25mesophilic
23325positivegrowth28mesophilic
23325positivegrowth33mesophilic
23325nogrowth42thermophilic
62590positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 62590
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.998

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2332517128adipate-assimilation
2332516947citrate-assimilation
2332517634D-glucose-assimilation
2332516899D-mannitol-assimilation
2332516024D-mannose-assimilation
2332530849L-arabinose-assimilation
2332525115malate-assimilation
2332517306maltose-assimilation
23325506227N-acetylglucosamine-assimilation
2332518401phenylacetate+assimilation
2332517632nitrate+reduction
2332532032potassium gluconate+/-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
233252676amoxicillinyesyes
2332517334penicillinyesyes
2332550845doxycyclineyesyes

enzymes

@refvalueactivityec
23325beta-glucosidase+3.2.1.21
23325catalase+1.11.1.6
23325cytochrome oxidase+1.9.3.1
23325urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentsampling date
18269root nodules of Robinia pseudoacaciaRobinia pseudoacaciaFlanders, Aalst, industrial areaBelgiumBELEurope
62590Root nodule,Robinia pseudoacaciaFlanders,AalstBelgiumBELEurope2008-09-04

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_7572.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_1319;97_1566;98_1905;99_7572&stattab=map
  • Last taxonomy: Bosea
  • 16S sequence: FR774994
  • Sequence Identity:
  • Total samples: 496
  • soil counts: 94
  • aquatic counts: 64
  • animal counts: 131
  • plant counts: 207

Safety information

risk assessment

  • @ref: 18269
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18269
  • description: Bosea sp. R-46070 partial 16S rRNA gene, strain R-46070
  • accession: FR774994
  • length: 1439
  • database: ena
  • NCBI tax ID: 1036780

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bosea robiniae DSM 26672GCA_900102525contigncbi1036780
66792Bosea robiniae strain DSM 266721036780.5wgspatric1036780
66792Bosea robiniae DSM 266722675903146draftimg1036780

GC content

  • @ref: 23325
  • GC-content: 65.0
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes91.474yes
flagellatedno60.871no
gram-positiveno98.384yes
anaerobicno98.027yes
aerobicyes94.523yes
halophileno93.447no
spore-formingno96.33no
thermophileno97.227no
glucose-utilyes85.47yes
glucose-fermentno90.396no

External links

@ref: 18269

culture collection no.: DSM 26672, CCUG 61249, LMG 26381

straininfo link

  • @ref: 71440
  • straininfo: 371082

literature

  • topic: Phylogeny
  • Pubmed-ID: 22155761
  • title: Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes.
  • authors: De Meyer SE, Willems A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.035477-0
  • year: 2011
  • mesh: Bacterial Typing Techniques, Bradyrhizobiaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fabaceae/*microbiology, Fatty Acids/analysis, Genes, Bacterial, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/microbiology, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
18269Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26672)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26672
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23325Sofie E. De Meyer, Anne Willems10.1099/ijs.0.035477-0Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumesIJSEM 62: 2505-2510 201222155761
62590Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 61249)https://www.ccug.se/strain?id=61249
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71440Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID371082.1StrainInfo: A central database for resolving microbial strain identifiers