Strain identifier
BacDive ID: 17928
Type strain: 
Species: Aquabacterium limnoticum
Strain Designation: ABP-4
Strain history: <- W-M Chen, Nat Kaohsiung Marine Uinv., Taiwan
NCBI tax ID(s): 708634 (species)
version 8.1 (current version)
General
@ref: 18224
BacDive-ID: 17928
DSM-Number: 26447
keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped
description: Aquabacterium limnoticum ABP-4 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from freshwater spring.
NCBI tax id
- NCBI tax id: 708634
- Matching level: species
strain history
| @ref | history |
|---|---|
| 18224 | <- KCTC <- S.-Y. Sheu, Dept. of Marine Biotechnology, Kaohsiung, Taiwan |
| 67771 | <- W-M Chen, Nat Kaohsiung Marine Uinv., Taiwan |
doi: 10.13145/bacdive17928.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Comamonadaceae
- genus: Aquabacterium
- species: Aquabacterium limnoticum
- full scientific name: Aquabacterium limnoticum Chen et al. 2012
@ref: 18224
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Comamonadaceae
genus: Aquabacterium
species: Aquabacterium limnoticum
full scientific name: Aquabacterium limnoticum Chen et al. 2012
strain designation: ABP-4
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility |
|---|---|---|---|---|---|
| 30203 | negative | 1.3-2.0 µm | 0.8-1 µm | rod-shaped | no |
| 67771 | negative |
multimedia
- @ref: 18224
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_26447.jpg
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
- @ref: 18224
- name: R2A MEDIUM (DSMZ Medium 830)
- growth: yes
- link: https://mediadive.dsmz.de/medium/830
- composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 18224 | positive | growth | 28 | mesophilic |
| 30203 | positive | growth | 20-40 | |
| 30203 | positive | optimum | 30-37 | mesophilic |
| 67771 | positive | growth | 25 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 30203 | positive | growth | 07-10 | alkaliphile |
| 30203 | positive | optimum | 07-09 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 30203 | facultative anaerobe |
| 67771 | aerobe |
spore formation
- @ref: 30203
- spore formation: no
halophily
- @ref: 30203
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-3 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30203 | 16449 | alanine | + | carbon source |
| 30203 | 22653 | asparagine | + | carbon source |
| 30203 | 29987 | glutamate | + | carbon source |
| 30203 | 28087 | glycogen | + | carbon source |
| 30203 | 51850 | methyl pyruvate | + | carbon source |
| 30203 | 30031 | succinate | + | carbon source |
| 30203 | 53426 | tween 80 | + | carbon source |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 30203 | alkaline phosphatase | + | 3.1.3.1 |
| 30203 | catalase | + | 1.11.1.6 |
| 30203 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 18224 | freshwater spring | Taichung County | Taiwan | TWN | Asia | 24.1208 | 120.674 |
| 67771 | From freshwater | Taiwan | TWN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Freshwater |
| #Environmental | #Aquatic | #Spring |
Safety information
risk assessment
- @ref: 18224
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 18224
- description: Aquabacterium limnoticum strain ABP-4 16S ribosomal RNA gene, partial sequence
- accession: GU319965
- length: 1443
- database: ena
- NCBI tax ID: 708634
GC content
- @ref: 18224
- GC-content: 68.6
External links
@ref: 18224
culture collection no.: DSM 26447, BCRC 80167, KCTC 23306
straininfo link
- @ref: 86917
- straininfo: 402256
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 21551326 | Aquabacterium limnoticum sp. nov., isolated from a freshwater spring. | Chen WM, Cho NT, Yang SH, Arun AB, Young CC, Sheu SY | Int J Syst Evol Microbiol | 10.1099/ijs.0.030635-0 | 2011 | Aerobiosis, Anaerobiosis, Bacterial Typing Techniques, Base Composition, Benzoquinones/analysis, Betaproteobacteria/*classification/genetics/*isolation & purification/physiology, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Natural Springs/*microbiology, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Taiwan, Temperature | Genetics |
| Phylogeny | 26297008 | Aquabacterium olei sp. nov., an oil-degrading bacterium isolated from oil-contaminated soil. | Pham VHT, Jeong SW, Kim J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000458 | 2015 | Bacterial Typing Techniques, Base Composition, Betaproteobacteria/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, *Petroleum Pollution, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants | Genetics |
| Phylogeny | 31622237 | Aquabacterium pictum sp. nov., the first aerobic bacteriochlorophyll a-containing fresh water bacterium in the genus Aquabacterium of the class Betaproteobacteria. | Hirose S, Tank M, Hara E, Tamaki H, Mori K, Takaichi S, Haruta S, Hanada S | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003798 | 2020 | Bacterial Typing Techniques, Bacteriochlorophyll A/*chemistry, Base Composition, Biofilms, Burkholderiales/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Japan, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry, Water Microbiology | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 18224 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26447) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26447 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30203 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26548 | 28776041 | |
| 67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
| 86917 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID402256.1 | StrainInfo: A central database for resolving microbial strain identifiers |