Strain identifier

BacDive ID: 1792

Type strain: Yes

Species: Bosea lupini

Strain Designation: R-45681

Strain history: <- LMG <- A. Willems and S. De Meyer, Group Biochemistry and Microbiology, University of Ghent

NCBI tax ID(s): 1036779 (species)

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General

@ref: 18270

BacDive-ID: 1792

DSM-Number: 26673

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Bosea lupini R-45681 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from root nodules of Lupinus polyphyllus.

NCBI tax id

  • NCBI tax id: 1036779
  • Matching level: species

strain history

  • @ref: 18270
  • history: <- LMG <- A. Willems and S. De Meyer, Group Biochemistry and Microbiology, University of Ghent

doi: 10.13145/bacdive1792.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Boseaceae
  • genus: Bosea
  • species: Bosea lupini
  • full scientific name: Bosea lupini De Meyer and Willems 2012

@ref: 18270

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Boseaceae

genus: Bosea

species: Bosea lupini

full scientific name: Bosea lupini De Meyer and Willems 2012

strain designation: R-45681

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23325negative1.2-1.5 µm0.8 µmrod-shapedyes
69480yes97.197
69480negative99.984

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
23325YMAyes
18270NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18270positivegrowth28mesophilic
23325positivegrowth25mesophilic
23325positivegrowth28mesophilic
23325positivegrowth33mesophilic
23325nogrowth42thermophilic
62589positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 62589
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.979

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2332516899D-mannitol-assimilation
2332517306maltose-assimilation
23325506227N-acetylglucosamine-assimilation
2332518401phenylacetate-assimilation
2332517632nitrate-reduction
2332525115malate+assimilation
2332532032potassium gluconate+assimilation
2332517128adipate+/-assimilation
2332516947citrate+/-assimilation
2332517634D-glucose+/-assimilation
2332516024D-mannose+/-assimilation
2332530849L-arabinose+/-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
233252676amoxicillinyesyes
2332517334penicillinyesyes
2332550845doxycyclineyesyes

enzymes

@refvalueactivityec
23325beta-glucosidase+3.2.1.21
23325catalase+1.11.1.6
23325cytochrome oxidase+1.9.3.1
23325urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
23325root nodules of Lupinus polyphyllusFlandersBelgiumBELEurope
62589Root nodule,Lupinus polyphyllusFlandersBelgiumBELEurope2008-09-10
18270root nodules of Robinia pseudoacaciagarden of the Ghent UniversityBelgiumBELEurope

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root nodule

Safety information

risk assessment

  • @ref: 18270
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18270
  • description: Bosea sp. R-45681 partial 16S rRNA gene, strain R-45681
  • accession: FR774992
  • length: 1444
  • database: ena
  • NCBI tax ID: 946785

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bosea lupini LMG 26383,CCUG 61248,R- 45681GCA_900109525contigncbi1036779
66792Bosea lupini strain LMG 26383,CCUG 61248,R- 456811036779.5wgspatric1036779
66792Bosea lupini DSM 266732634166302draftimg1036779

GC content

  • @ref: 23325
  • GC-content: 66.9
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes89.568yes
flagellatedno59.436no
gram-positiveno98.365yes
anaerobicno98.553yes
aerobicyes94.815yes
halophileno94.622no
spore-formingno94.321no
thermophileno97.504no
glucose-utilyes92.534no
glucose-fermentno92.275no

External links

@ref: 18270

culture collection no.: DSM 26673, CCUG 61248, LMG 26383

straininfo link

  • @ref: 71439
  • straininfo: 371081

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22155761Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes.De Meyer SE, Willems AInt J Syst Evol Microbiol10.1099/ijs.0.035477-02011Bacterial Typing Techniques, Bradyrhizobiaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fabaceae/*microbiology, Fatty Acids/analysis, Genes, Bacterial, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/microbiology, Sequence Analysis, DNATranscriptome
Phylogeny30882299Bosea psychrotolerans sp. nov., a psychrotrophic alphaproteobacterium isolated from Lake Michigan water.Albert RA, McGuine M, Pavlons SC, Roecker J, Bruess J, Mossman S, Sun S, King M, Hong S, Farrance CE, Danner J, Joung Y, Shapiro N, Whitman WB, Busse HJInt J Syst Evol Microbiol10.1099/ijsem.0.0033192019Bacterial Typing Techniques, Base Composition, Bradyrhizobiaceae/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Lakes/*microbiology, Michigan, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spermidine/analogs & derivatives/chemistry, Ubiquinone/chemistryTranscriptome
Phylogeny31166161Bosea caraganae sp. nov. a new species of slow-growing bacteria isolated from root nodules of the relict species Caragana jubata (Pall.) Poir. originating from Mongolia.Sazanova AL, Safronova VI, Kuznetsova IG, Karlov DS, Belimov AA, Andronov EE, Chirak ER, Popova JP, Verkhozina AV, Willems A, Tikhonovich IAInt J Syst Evol Microbiol10.1099/ijsem.0.0035092019Bacterial Typing Techniques, Base Composition, Bradyrhizobiaceae/*classification/isolation & purification, Caragana/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Mongolia, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, SymbiosisTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
18270Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26673)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26673
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23325Sofie E. De Meyer, Anne Willems10.1099/ijs.0.035477-0Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov., Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumesIJSEM 62: 2505-2510 201222155761
62589Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 61248)https://www.ccug.se/strain?id=61248
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71439Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID371081.1StrainInfo: A central database for resolving microbial strain identifiers