Archive BacDiveID:10.13145/bacdive17902.20230509.8.1

Strain identifier

BacDive ID: 17902

Type strain:

Species: Methylonatrum kenyense

Strain history: <- D. Y. Sorokin, Winogradski Inst. Microbiol., RAS, Moscow, Russia; AMT 1

NCBI tax ID(s): 455253 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 6089

BacDive-ID: 17902

DSM-Number: 15732

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Methylonatrum kenyense DSM 15732 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from soda lake Magadi.

NCBI tax id

NCBI tax id: 455253
Matching level: species

strain history

@ref: 6089
history: <- D. Y. Sorokin, Winogradski Inst. Microbiol., RAS, Moscow, Russia; AMT 1

doi: 10.13145/bacdive17902.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Gammaproteobacteria
order: Chromatiales
family: Ectothiorhodospiraceae
genus: Methylonatrum
species: Methylonatrum kenyense
full scientific name: Methylonatrum kenyense Sorokin et al. 2007

@ref: 6089

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Ectothiorhodospiraceae

genus: Methylonatrum

species: Methylonatrum kenyense

full scientific name: Methylonatrum kenyense Sorokin et al. 2007

type strain: yes

Morphology

cell morphology

@ref: 32094
gram stain: negative
cell length: 1.1 µm
cell width: 0.6 µm
cell shape: rod-shaped
motility: no

Culture and growth conditions

culture medium

@ref: 6089
name: METHYLONATRUM MEDIUM (DSMZ Medium 1170)
growth: yes
link: https://mediadive.dsmz.de/medium/1170
composition: Name: METHYLONATRUM MEDIUM (DSMZ Medium 1170) Composition: NaCl 18.0 g/l Na2CO3 11.0 g/l Methanol 1.584 g/l K2HPO4 1.0 g/l KNO3 1.0 g/l MgSO4 x 7 H2O 0.24 g/l NH4Cl 0.16 g/l Vitamin B12 0.1 g/l NaS2O3 x 5 H2O 0.024 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water

culture temp

@ref: 6089
growth: positive
type: growth
temperature: 28
range: mesophilic

culture pH

@ref	ability	type	pH	PH range
32094	positive	growth	8.3-10.5	alkaliphile
32094	positive	optimum	10

Physiology and metabolism

oxygen tolerance

@ref: 32094
oxygen tolerance: aerobe

halophily

@ref	salt	growth	tested relation	concentration
32094	NaCl	positive	growth	1.74-23.2 %
32094	NaCl	positive	optimum	2.9 %

observation

@ref: 32094
observation: aggregates in clumps

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
32094	30089	acetate	+	carbon source
32094	16236	ethanol	+	carbon source
32094	15740	formate	+	carbon source

Isolation, sampling and environmental information

isolation

@ref: 6089
sample type: soda lake Magadi
country: Kenya
origin.country: KEN
continent: Africa

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Condition	#Alkaline

taxonmaps

@ref: 69479
File name: preview.99_18417.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_56;96_9403;97_11309;98_13908;99_18417&stattab=map
Last taxonomy: Gammaproteobacteria
16S sequence: DQ789390
Sequence Identity:
Total samples: 155
soil counts: 55
aquatic counts: 85
animal counts: 14
plant counts: 1

Safety information

risk assessment

@ref: 6089
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 6089
description: Methylonatrum kenyense strain AMT 1 16S ribosomal RNA gene, partial sequence
accession: DQ789390
length: 1318
database: ena
NCBI tax ID: 455253

GC content

@ref: 32094
GC-content: 62.9

External links

@ref: 6089

culture collection no.: DSM 15732, NCCB 100209, UNIQEM U238, AMT 1

straininfo link

@ref: 86891
straininfo: 401486

literature

topic: Metabolism
Pubmed-ID: 18048722
title: Methylohalomonas lacus gen. nov., sp. nov. and Methylonatrum kenyense gen. nov., sp. nov., methylotrophic gammaproteobacteria from hypersaline lakes.
authors: Sorokin DY, Trotsenko YA, Doronina NV, Tourova TP, Galinski EA, Kolganova TV, Muyzer G
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijs.0.64955-0
year: 2007
mesh: Aerobiosis/physiology, Betaine/analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Dimethylamines/metabolism, Ectothiorhodospiraceae/genetics, Ethanol/metabolism, Formaldehyde/metabolism, Formates/metabolism, Gammaproteobacteria/chemistry/*classification/genetics/*isolation & purification, Genes, rRNA, Hydrogen-Ion Concentration, Kenya, Methanol/*metabolism, Methylamines/metabolism, Molecular Sequence Data, Oxidation-Reduction, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, *Water Microbiology
topic2: Phylogeny

Reference

@id	authors	catalogue	doi/url	title	ID_cross_reference	pubmed
6089	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15732)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-15732
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32094	Barberan A, Caceres Velazquez H, Jones S, Fierer N.		10.1128/mSphere.00237-17	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information	28340	28776041
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
86891	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID401486.1	StrainInfo: A central database for resolving microbial strain identifiers