Strain identifier
BacDive ID: 17900
Type strain:
Species: Eionea nigra
Strain Designation: 17X/A02/237
Strain history: CIP <- 2007, L. Urios, Paris Univ. VI, CNRS, Banyuls sur mer, France, strain: 17X/A02/237
NCBI tax ID(s): 287488 (species)
General
@ref: 8292
BacDive-ID: 17900
DSM-Number: 19752
keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped
description: Eionea nigra 17X/A02/237 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from coastal waters.
NCBI tax id
- NCBI tax id: 287488
- Matching level: species
strain history
@ref | history |
---|---|
8292 | <- P. Lebaron; 17X/A02/237 <- L. Intertaglia |
42015 | 2007, L. Urios, Paris Univ. VI, CNRS, Banyuls sur mer, France, strain: 17X/A02/237 |
121295 | CIP <- 2007, L. Urios, Paris Univ. VI, CNRS, Banyuls sur mer, France, strain: 17X/A02/237 |
doi: 10.13145/bacdive17900.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Cellvibrionales
- family: Cellvibrionaceae
- genus: Eionea
- species: Eionea nigra
- full scientific name: Eionea nigra Urios et al. 2011
@ref: 8292
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Cellvibrionaceae, not assigned to order
family: Cellvibrionaceae
genus: Eionea
species: Eionea nigra
full scientific name: Eionea nigra Urios et al. 2011
strain designation: 17X/A02/237
type strain: yes
Morphology
cell morphology
- @ref: 121295
- gram stain: negative
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8292 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
42015 | Marine agar (MA) | yes | Distilled water make up to (1000.000 ml);Marine agar (55.100 g) | |
121295 | CIP Medium 13 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8292 | positive | growth | 28 | mesophilic |
42015 | positive | growth | 30 | mesophilic |
Physiology and metabolism
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
121295 | 17632 | nitrate | - | reduction |
121295 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 121295
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
121295 | oxidase | - | |
121295 | catalase | - | 1.11.1.6 |
121295 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121295 | - | + | + | + | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | isolation date |
---|---|---|---|---|---|---|---|---|
8292 | coastal waters | Mediterranean Sea, bay of Banyuls-sur-Mer (42° 29' N 3° 08' E) | France | FRA | Europe | 42.4833 | 3.1333 | |
121295 | Environment, Mediterranean sea | Banyuls sur mer | France | FRA | Europe | 2002 |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Marine
taxonmaps
- @ref: 69479
- File name: preview.99_21153.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_235;96_10728;97_12970;98_15958;99_21153&stattab=map
- Last taxonomy: Eionea nigra subclade
- 16S sequence: AY576771
- Sequence Identity:
- Total samples: 2868
- soil counts: 25
- aquatic counts: 2676
- animal counts: 159
- plant counts: 8
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
8292 | 1 | Risk group (German classification) |
121295 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 8292
- description: Eionea nigra strain 17X/A02/237 16S ribosomal RNA gene, partial sequence
- accession: AY576771
- length: 1463
- database: ena
- NCBI tax ID: 287488
GC content
- @ref: 8292
- GC-content: 47.5
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 8292
culture collection no.: DSM 19752, CIP 109759, MOLA 288
straininfo link
- @ref: 86889
- straininfo: 377748
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 20729310 | Eionea nigra gen. nov., sp. nov., a gammaproteobacterium from the Mediterranean Sea. | Urios L, Intertaglia L, Lesongeur F, Lebaron P | Int J Syst Evol Microbiol | 10.1099/ijs.0.023952-0 | 2010 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/genetics/isolation & purification, Mediterranean Sea, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry | Genetics |
Phylogeny | 26025942 | Eionea flava sp. nov., isolated from coastal seawater, and emended description of the genus Eionea. | Baek K, Choi A, Cho JC | Int J Syst Evol Microbiol | 10.1099/ijs.0.000366 | 2015 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry, Water Microbiology | Genetics |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
8292 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19752) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-19752 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
42015 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7538 | ||
68382 | Automatically annotated from API zym | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
86889 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID377748.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
121295 | Curators of the CIP | Collection of Institut Pasteur (CIP 109759) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109759 |