Strain identifier
BacDive ID: 1788
Type strain:
Species: Bosea eneae
Strain Designation: 34614
Strain history: CIP <- 1999, B. La Scola, NRC, Fac. Medicine, Marseille, France: strain 34614
NCBI tax ID(s): 151454 (species)
General
@ref: 15778
BacDive-ID: 1788
DSM-Number: 21596
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming
description: Bosea eneae 34614 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from hospital water supplies of La Timone Hospital Centre.
NCBI tax id
- NCBI tax id: 151454
- Matching level: species
strain history
@ref | history |
---|---|
15778 | <- CCUG <- B. La Scola, CNRS, Marseille; 34614 |
39499 | 1999, B. La Scola, NRC, Fac. Medicine, Marseille, France: strain 34614 |
119032 | CIP <- 1999, B. La Scola, NRC, Fac. Medicine, Marseille, France: strain 34614 |
doi: 10.13145/bacdive1788.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Boseaceae
- genus: Bosea
- species: Bosea eneae
- full scientific name: Bosea eneae La Scola et al. 2003
@ref: 15778
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Boseaceae
genus: Bosea
species: Bosea eneae
full scientific name: Bosea eneae La Scola et al. 2003
strain designation: 34614
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell shape | motility | flagellum arrangement |
---|---|---|---|---|
23324 | negative | rod-shaped | yes | monotrichous, polar |
119032 | negative | rod-shaped | yes |
colony morphology
@ref | type of hemolysis | hemolysis ability | colony color | colony shape | incubation period | medium used |
---|---|---|---|---|---|---|
23324 | alpha | 1 | cream | circular | 2-3 days | BCYE agar |
55668 | 2-3 days |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
15778 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
23324 | BCYE agar | yes | ||
23324 | Columbia agar | yes | 5% sheep blood, 0.2% yeast extract | |
23324 | MacConkey's agar | yes | ||
23324 | NB | yes | ||
39499 | MEDIUM 23 - for Afipia and Legionella | yes | Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml) | |
119032 | CIP Medium 23 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
15778 | positive | growth | 28 | mesophilic |
23324 | positive | growth | 25 | mesophilic |
23324 | positive | growth | 37 | mesophilic |
23324 | no | growth | 42 | thermophilic |
39499 | positive | growth | 30 | mesophilic |
55668 | positive | growth | 30 | mesophilic |
119032 | positive | growth | 22-30 | |
119032 | no | growth | 15 | psychrophilic |
119032 | no | growth | 37 | mesophilic |
119032 | no | growth | 41 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 55668
- oxygen tolerance: aerobe
halophily
- @ref: 23324
- salt: NaCl
- growth: no
- tested relation: growth
- concentration: 6 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23324 | 22599 | arabinose | - | assimilation |
23324 | 16947 | citrate | - | assimilation |
23324 | 17634 | D-glucose | - | assimilation |
23324 | 25115 | malate | - | assimilation |
23324 | 17306 | maltose | - | assimilation |
23324 | 29864 | mannitol | - | assimilation |
23324 | 37684 | mannose | - | assimilation |
23324 | 506227 | N-acetylglucosamine | - | assimilation |
23324 | 18401 | phenylacetate | - | assimilation |
23324 | 16808 | 2-dehydro-D-gluconate | - | builds acid from |
23324 | 17426 | 5-dehydro-D-gluconate | - | builds acid from |
23324 | 27613 | amygdalin | - | builds acid from |
23324 | 18305 | arbutin | - | builds acid from |
23324 | 15963 | ribitol | - | builds acid from |
23324 | 17108 | D-arabinose | - | builds acid from |
23324 | 18333 | D-arabitol | - | builds acid from |
23324 | 17057 | cellobiose | - | builds acid from |
23324 | 15824 | D-fructose | - | builds acid from |
23324 | 28847 | D-fucose | - | builds acid from |
23324 | 12936 | D-galactose | - | builds acid from |
23324 | 17634 | D-glucose | - | builds acid from |
23324 | 17716 | lactose | - | builds acid from |
23324 | 62318 | D-lyxose | - | builds acid from |
23324 | 17306 | maltose | - | builds acid from |
23324 | 16899 | D-mannitol | - | builds acid from |
23324 | 16024 | D-mannose | - | builds acid from |
23324 | 6731 | melezitose | - | builds acid from |
23324 | 28053 | melibiose | - | builds acid from |
23324 | 16634 | raffinose | - | builds acid from |
23324 | 16988 | D-ribose | - | builds acid from |
23324 | 17992 | sucrose | - | builds acid from |
23324 | 17924 | D-sorbitol | - | builds acid from |
23324 | 16443 | D-tagatose | - | builds acid from |
23324 | 16551 | D-trehalose | - | builds acid from |
23324 | 32528 | turanose | - | builds acid from |
23324 | 65327 | D-xylose | - | builds acid from |
23324 | 16813 | galactitol | - | builds acid from |
23324 | 17113 | erythritol | - | builds acid from |
23324 | 4853 | esculin | - | builds acid from |
23324 | 28066 | gentiobiose | - | builds acid from |
23324 | 24265 | gluconate | - | builds acid from |
23324 | 17754 | glycerol | - | builds acid from |
23324 | 28087 | glycogen | - | builds acid from |
23324 | 17268 | myo-inositol | - | builds acid from |
23324 | 15443 | inulin | - | builds acid from |
23324 | 30849 | L-arabinose | - | builds acid from |
23324 | 18403 | L-arabitol | - | builds acid from |
23324 | 18287 | L-fucose | - | builds acid from |
23324 | 62345 | L-rhamnose | - | builds acid from |
23324 | 17266 | L-sorbose | - | builds acid from |
23324 | 65328 | L-xylose | - | builds acid from |
23324 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
23324 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
23324 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
23324 | 506227 | N-acetylglucosamine | - | builds acid from |
23324 | 17814 | salicin | - | builds acid from |
23324 | 28017 | starch | - | builds acid from |
23324 | 17151 | xylitol | - | builds acid from |
23324 | 4853 | esculin | - | hydrolysis |
23324 | 5291 | gelatin | - | hydrolysis |
23324 | 16094 | thiosulfate | - | oxidation |
23324 | 17632 | nitrate | - | reduction |
23324 | 17128 | adipate | + | assimilation |
23324 | 24265 | gluconate | + | assimilation |
119032 | 606565 | hippurate | + | hydrolysis |
119032 | 17632 | nitrate | - | reduction |
119032 | 16301 | nitrite | - | reduction |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. | group ID | is resistant |
---|---|---|---|---|---|---|---|
23324 | 50845 | doxycycline | yes | yes | 1 µg/mL (MIC) | ||
23324 | 3542 | cephalothin | yes | yes | 128 µg/mL (MIC) | ||
23324 | 17833 | gentamicin | yes | yes | 128 µg/mL (MIC) | ||
23324 | 209807 | cefoxitin | yes | yes | 2 µg/mL (MIC) | ||
23324 | 7528 | netilmycin | yes | yes | 2 µg/mL (MIC) | ||
23324 | 2676 | amoxicillin | yes | yes | 256 µg/mL (MIC) | ||
23324 | 478164 | cefepime | yes | yes | 256 µg/mL (MIC) | ||
23324 | 3508 | ceftazidime | yes | yes | 256 µg/mL (MIC) | ||
23324 | 29007 | ceftriaxone | yes | yes | 256 µg/mL (MIC) | ||
23324 | 100241 | ciprofloxacin | yes | yes | 256 µg/mL (MIC) | ||
23324 | 3770 | co-trimoxazole | yes | yes | 256 µg/mL (MIC) | ||
23324 | 37943 | colistin | yes | yes | 256 µg/mL (MIC) | ||
23324 | 17334 | penicillin | yes | yes | 256 µg/mL (MIC) | ||
23324 | 8232 | piperacillin | yes | yes | 256 µg/mL (MIC) | ||
23324 | 9587 | ticarcillin | yes | yes | 256 µg/mL (MIC) | ||
23324 | 28001 | vancomycin | yes | yes | 256 µg/mL (MIC) | ||
23324 | 2676 | amoxicillin | yes | yes | 32 µg/mL (MIC) | 12 | |
23324 | 28077 | rifampicin | yes | yes | 4 µg/mL (MIC) | ||
23324 | 48923 | erythromycin | yes | yes | 64 µg/mL (MIC) | ||
23324 | 471744 | imipenem | yes | yes | 64 µg/mL (MIC) | ||
23324 | 28864 | tobramycin | yes | yes | 64 µg/mL (MIC) | ||
23324 | 2637 | amikacin | yes | yes | 8 µg/mL (MIC) | ||
23324 | 48947 | clavulanic acid | yes | yes | 32 µg/mL (MIC) | 12 | |
119032 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) | yes | no | yes |
metabolite production
- @ref: 119032
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
23324 | arginine dihydrolase | - | 3.5.3.6 |
23324 | beta-galactosidase | - | 3.2.1.23 |
23324 | catalase | + | 1.11.1.6 |
23324 | cytochrome oxidase | + | 1.9.3.1 |
23324 | urease | + | 3.5.1.5 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | - | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
119032 | oxidase | + | |
119032 | alcohol dehydrogenase | - | 1.1.1.1 |
119032 | gelatinase | - | |
119032 | catalase | + | 1.11.1.6 |
119032 | gamma-glutamyltransferase | - | 2.3.2.2 |
119032 | lysine decarboxylase | - | 4.1.1.18 |
119032 | ornithine decarboxylase | - | 4.1.1.17 |
119032 | urease | + | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
119032 | - | + | + | + | - | - | - | - | - | - | - | + | - | - | - | + | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
15778 | hospital water supplies of La Timone Hospital Centre | Marseilles | France | FRA | Europe |
55668 | Water,hospital water supply | ||||
119032 | Environment, Hospital water supplies |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Freshwater |
#Infection | #Medical environment | #Clinic |
taxonmaps
- @ref: 69479
- File name: preview.99_3093.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_1319;97_1566;98_1905;99_3093&stattab=map
- Last taxonomy: Bosea
- 16S sequence: AF288300
- Sequence Identity:
- Total samples: 1248
- soil counts: 165
- aquatic counts: 435
- animal counts: 235
- plant counts: 413
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
15778 | 1 | Risk group (German classification) |
119032 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 15778
- description: Alpha proteobacterium 34614 16S ribosomal RNA gene, partial sequence
- accession: AF288300
- length: 1446
- database: ena
- NCBI tax ID: 1211774
GC content
- @ref: 23324
- GC-content: 69.0
- method: high performance liquid chromatography (HPLC)
External links
@ref: 15778
culture collection no.: DSM 21596, CCUG 43111, CIP 106338
straininfo link
- @ref: 71435
- straininfo: 100565
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 12656146 | Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996). | La Scola B, Mallet MN, Grimont PAD, Raoult D | Int J Syst Evol Microbiol | 10.1099/ijs.0.02127-0 | 2003 | Alphaproteobacteria/*classification/genetics/isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Hospitals, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, *Water Microbiology | Genetics |
Phylogeny | 30882299 | Bosea psychrotolerans sp. nov., a psychrotrophic alphaproteobacterium isolated from Lake Michigan water. | Albert RA, McGuine M, Pavlons SC, Roecker J, Bruess J, Mossman S, Sun S, King M, Hong S, Farrance CE, Danner J, Joung Y, Shapiro N, Whitman WB, Busse HJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003319 | 2019 | Bacterial Typing Techniques, Base Composition, Bradyrhizobiaceae/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Lakes/*microbiology, Michigan, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spermidine/analogs & derivatives/chemistry, Ubiquinone/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
15778 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21596) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21596 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23324 | Bernard La Scola, Marie-Noelle Mallet, Patrick A. D. Grimont, Didier Raoult | 10.1099/ijs.0.02127-0 | Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996) | IJSEM 53: 15-20 2003 | 12656146 | |
39499 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/18458 | ||||
55668 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 43111) | https://www.ccug.se/strain?id=43111 | |||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
71435 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID100565.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119032 | Curators of the CIP | Collection of Institut Pasteur (CIP 106338) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106338 |