Strain identifier
BacDive ID: 1787
Type strain:
Species: Bosea minatitlanensis
Strain history: CIP <- 2000, H. Macarie, U.A.M.I., Mexico: strain AMX 51 <- S. Thierry
NCBI tax ID(s): 128782 (species)
General
@ref: 4903
BacDive-ID: 1787
DSM-Number: 13099
keywords: 16S sequence, Bacteria, facultative aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming
description: Bosea minatitlanensis CCUG 55772 is a facultative aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from industrial waste water.
NCBI tax id
- NCBI tax id: 128782
- Matching level: species
strain history
@ref | history |
---|---|
4903 | <- A. S. Ouattara, IRD; AMX 51 <- S. Thierry |
120956 | CIP <- 2000, H. Macarie, U.A.M.I., Mexico: strain AMX 51 <- S. Thierry |
doi: 10.13145/bacdive1787.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Boseaceae
- genus: Bosea
- species: Bosea minatitlanensis
- full scientific name: Bosea minatitlanensis Ouattara et al. 2003
@ref: 4903
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Boseaceae
genus: Bosea
species: Bosea minatitlanensis
full scientific name: Bosea minatitlanensis Ouattara et al. 2003
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | flagellum arrangement |
---|---|---|---|---|---|---|
23323 | negative | 1.5-2.0 µm | 0.5 µm | rod-shaped | yes | monotrichous, polar |
120956 | negative | rod-shaped | yes |
colony morphology
- @ref: 23323
- colony shape: circular
- incubation period: 2-10 days
- medium used: Trypticase soy
pigmentation
- @ref: 120956
- production: no
- name: Pyocyanin
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
4903 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
4903 | BCYE-AGAR (DSMZ Medium 585) | yes | https://mediadive.dsmz.de/medium/585 | Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base |
23323 | Trypticase soy | yes | ||
39533 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
120956 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
4903 | positive | growth | 37 | mesophilic |
23323 | positive | growth | 15-42 | |
23323 | no | growth | 4 | psychrophilic |
23323 | no | growth | 45 | thermophilic |
23323 | positive | optimum | 37 | mesophilic |
39533 | positive | growth | 30 | mesophilic |
60723 | positive | growth | 30-37 | mesophilic |
120956 | positive | growth | 25-41 | |
120956 | no | growth | 5 | psychrophilic |
120956 | no | growth | 10 | psychrophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
23323 | positive | growth | 5.0-8.0 |
23323 | positive | optimum | 6.0 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
23323 | facultative aerobe |
60723 | aerobe |
120956 | obligate aerobe |
spore formation
- @ref: 23323
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
120956 | NaCl | positive | growth | 0-4 % |
120956 | NaCl | no | growth | 6 % |
120956 | NaCl | no | growth | 8 % |
120956 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23323 | 16808 | 2-dehydro-D-gluconate | - | builds acid from |
23323 | 17426 | 5-dehydro-D-gluconate | - | builds acid from |
23323 | 27613 | amygdalin | - | builds acid from |
23323 | 18305 | arbutin | - | builds acid from |
23323 | 15963 | ribitol | - | builds acid from |
23323 | 17108 | D-arabinose | - | builds acid from |
23323 | 18333 | D-arabitol | - | builds acid from |
23323 | 17057 | cellobiose | - | builds acid from |
23323 | 15824 | D-fructose | - | builds acid from |
23323 | 28847 | D-fucose | - | builds acid from |
23323 | 12936 | D-galactose | - | builds acid from |
23323 | 17634 | D-glucose | - | builds acid from |
23323 | 17716 | lactose | - | builds acid from |
23323 | 62318 | D-lyxose | - | builds acid from |
23323 | 17306 | maltose | - | builds acid from |
23323 | 16899 | D-mannitol | - | builds acid from |
23323 | 16024 | D-mannose | - | builds acid from |
23323 | 6731 | melezitose | - | builds acid from |
23323 | 28053 | melibiose | - | builds acid from |
23323 | 16634 | raffinose | - | builds acid from |
23323 | 16988 | D-ribose | - | builds acid from |
23323 | 17992 | sucrose | - | builds acid from |
23323 | 17924 | D-sorbitol | - | builds acid from |
23323 | 16443 | D-tagatose | - | builds acid from |
23323 | 16551 | D-trehalose | - | builds acid from |
23323 | 32528 | turanose | - | builds acid from |
23323 | 65327 | D-xylose | - | builds acid from |
23323 | 16813 | galactitol | - | builds acid from |
23323 | 17113 | erythritol | - | builds acid from |
23323 | 4853 | esculin | - | builds acid from |
23323 | 28066 | gentiobiose | - | builds acid from |
23323 | 24265 | gluconate | - | builds acid from |
23323 | 17754 | glycerol | - | builds acid from |
23323 | 28087 | glycogen | - | builds acid from |
23323 | 17268 | myo-inositol | - | builds acid from |
23323 | 15443 | inulin | - | builds acid from |
23323 | 30849 | L-arabinose | - | builds acid from |
23323 | 18403 | L-arabitol | - | builds acid from |
23323 | 18287 | L-fucose | - | builds acid from |
23323 | 62345 | L-rhamnose | - | builds acid from |
23323 | 17266 | L-sorbose | - | builds acid from |
23323 | 65328 | L-xylose | - | builds acid from |
23323 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
23323 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
23323 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
23323 | 506227 | N-acetylglucosamine | - | builds acid from |
23323 | 17814 | salicin | - | builds acid from |
23323 | 28017 | starch | - | builds acid from |
23323 | 17151 | xylitol | - | builds acid from |
23323 | 16947 | citrate | - | carbon source |
23323 | 16808 | 2-dehydro-D-gluconate | + | growth |
23323 | 30916 | 2-oxoglutarate | + | growth |
23323 | 37054 | 3-hydroxybutyrate | + | growth |
23323 | 16865 | gamma-aminobutyric acid | + | growth |
23323 | 30089 | acetate | + | growth |
23323 | 35899 | crotonate | + | growth |
23323 | 18024 | D-galacturonic acid | + | growth |
23323 | 8391 | D-gluconate | + | growth |
23323 | 15748 | D-glucuronate | + | growth |
23323 | 15588 | D-malate | + | growth |
23323 | 33871 | glycerate | + | growth |
23323 | 24996 | lactate | + | growth |
23323 | 15740 | formate | + | growth |
23323 | 17859 | glutaric acid | + | growth |
23323 | 16977 | L-alanine | + | growth |
23323 | 16467 | L-arginine | + | growth |
23323 | 17196 | L-asparagine | + | growth |
23323 | 17561 | L-cysteine | + | growth |
23323 | 29985 | L-glutamate | + | growth |
23323 | 18050 | L-glutamine | + | growth |
23323 | 15589 | L-malate | + | growth |
23323 | 17295 | L-phenylalanine | + | growth |
23323 | 17203 | L-proline | + | growth |
23323 | 17790 | methanol | + | growth |
23323 | 15361 | pyruvate | + | growth |
23323 | 16094 | thiosulfate | + | oxidation |
120956 | 16947 | citrate | - | carbon source |
120956 | 4853 | esculin | - | hydrolysis |
120956 | 17632 | nitrate | - | reduction |
120956 | 16301 | nitrite | - | reduction |
120956 | 17632 | nitrate | - | respiration |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. | group ID | is intermediate | intermediate conc. | is sensitive | sensitivity conc. |
---|---|---|---|---|---|---|---|---|---|---|
23323 | 3508 | ceftazidime | yes | yes | 30 µg | |||||
23323 | 100241 | ciprofloxacin | yes | yes | 5 µg | |||||
23323 | 7731 | ofloxacin | yes | yes | 5 µg | |||||
23323 | 2676 | amoxicillin | yes | 11 | yes | |||||
23323 | 8232 | piperacillin | yes | yes | 75 µg | |||||
23323 | 17833 | gentamicin | yes | yes | 10 Unit | |||||
23323 | 37943 | colistin | yes | yes | 300 Unit | |||||
23323 | 471744 | imipenem | yes | yes | 10 µg | |||||
23323 | 28864 | tobramycin | yes | yes | 10 µg | |||||
23323 | 2637 | amikacin | yes | yes | 30 µg | |||||
23323 | 3542 | cephalothin | yes | yes | 30 µg | |||||
23323 | 204928 | cefotaxime | yes | yes | 30 µg | |||||
23323 | 7528 | netilmycin | yes | yes | 30 µg | |||||
23323 | 9587 | ticarcillin | yes | yes | 75 µg | |||||
23323 | 3770 | co-trimoxazole | yes | yes | ||||||
23323 | 8232 | piperacillin | yes | 16 | yes | |||||
23323 | 48947 | clavulanic acid | yes | 11 | yes | |||||
23323 | 9421 | tazobactam | yes | 16 | yes | |||||
120956 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) | yes | yes | no |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
23323 | 35581 | indole | no |
120956 | 35581 | indole | no |
metabolite tests
- @ref: 23323
- Chebi-ID: 16947
- metabolite: citrate
- citrate test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
23323 | amylase | +/- | |
23323 | arginine dihydrolase | - | 3.5.3.6 |
23323 | beta-galactosidase | - | 3.2.1.23 |
23323 | catalase | + | 1.11.1.6 |
23323 | cytochrome oxidase | + | 1.9.3.1 |
23323 | DNase | - | |
23323 | esterase | - | |
23323 | lysine decarboxylase | - | 4.1.1.18 |
23323 | nitrate reductase | - | 1.7.99.4 |
23323 | nitrite reductase | - | 1.7.1.4 |
23323 | urease | + | 3.5.1.5 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | + | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
120956 | oxidase | + | |
120956 | beta-galactosidase | - | 3.2.1.23 |
120956 | alcohol dehydrogenase | - | 1.1.1.1 |
120956 | gelatinase | +/- | |
120956 | amylase | + | |
120956 | DNase | - | |
120956 | caseinase | - | 3.4.21.50 |
120956 | catalase | + | 1.11.1.6 |
120956 | tween esterase | - | |
120956 | lecithinase | - | |
120956 | lipase | - | |
120956 | lysine decarboxylase | - | 4.1.1.18 |
120956 | ornithine decarboxylase | - | 4.1.1.17 |
120956 | protease | - | |
120956 | tryptophan deaminase | - | |
120956 | urease | + | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120956 | - | + | - | + | - | + | + | - | + | - | + | + | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120956 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | + | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | + | - | + | + | - | - | - | - | - | + | - | + | + | - | + | - | - | - | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
4903 | industrial waste water | Iztapalapa | Mexico | MEX | North America | |
23323 | anaerobic sludge of a lab-scale UASB reactor treating the petrochemical wastewater of a purified terephthalic acid plant | |||||
60723 | Industrial waist water | Mexico | MEX | North America | ||
120956 | Environment, Mud | Mexico | MEX | North America | 1999 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Biodegradation | #Anaerobic digestor |
#Engineered | #Waste | #Wastewater |
#Environmental | #Terrestrial | #Mud (Sludge) |
#Condition | #Anoxic (anaerobic) | |
#Engineered | #Waste | #Industrial wastewater |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
4903 | 1 | Risk group (German classification) |
120956 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 4903
- description: Bosea minatitlanensis 16S ribosomal RNA gene, partial sequence
- accession: AF273081
- length: 1482
- database: ena
- NCBI tax ID: 128782
GC content
- @ref: 4903
- GC-content: 68.5
External links
@ref: 4903
culture collection no.: CCUG 55772, DSM 13099, ATCC 700918, CIP 106457, AMX 51
straininfo link
- @ref: 71434
- straininfo: 44209
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 13130002 | Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester. | Ouattara AS, Assih EA, Thierry S, Cayol JL, Labat M, Monroy O, Macarie H | Int J Syst Evol Microbiol | 10.1099/ijs.0.02540-0 | 2003 | Aerobiosis, Bradyrhizobiaceae/classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Genes, Bacterial, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sewage/microbiology, Species Specificity | Genetics |
Phylogeny | 24277862 | Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant. | Toth EM, Vengring A, Homonnay ZG, Keki Z, Sproer C, Borsodi AK, Marialigeti K, Schumann P | Int J Syst Evol Microbiol | 10.1099/ijs.0.053843-0 | 2013 | Alphaproteobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hungary, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, *Power Plants, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry, *Water Microbiology, *Water Purification | Enzymology |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
4903 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13099) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13099 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23323 | Aboubakar S. Ouattara, Essokazi A. Assih, Sébastien Thierry, Jean-Luc Cayol, Marc Labat, Oscar Monroy, Hervé Macarie | 10.1099/ijs.0.02540-0 | Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester | IJSEM 53: 1247-1251 2003 | 13130002 | |
39533 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/18590 | ||||
60723 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 55772) | https://www.ccug.se/strain?id=55772 | |||
68382 | Automatically annotated from API zym | |||||
71434 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44209.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120956 | Curators of the CIP | Collection of Institut Pasteur (CIP 106457) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106457 |