Strain identifier

BacDive ID: 1787

Type strain: Yes

Species: Bosea minatitlanensis

Strain history: CIP <- 2000, H. Macarie, U.A.M.I., Mexico: strain AMX 51 <- S. Thierry

NCBI tax ID(s): 128782 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4903

BacDive-ID: 1787

DSM-Number: 13099

keywords: 16S sequence, Bacteria, facultative aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Bosea minatitlanensis CCUG 55772 is a facultative aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from industrial waste water.

NCBI tax id

  • NCBI tax id: 128782
  • Matching level: species

strain history

@refhistory
4903<- A. S. Ouattara, IRD; AMX 51 <- S. Thierry
120956CIP <- 2000, H. Macarie, U.A.M.I., Mexico: strain AMX 51 <- S. Thierry

doi: 10.13145/bacdive1787.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Boseaceae
  • genus: Bosea
  • species: Bosea minatitlanensis
  • full scientific name: Bosea minatitlanensis Ouattara et al. 2003

@ref: 4903

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Boseaceae

genus: Bosea

species: Bosea minatitlanensis

full scientific name: Bosea minatitlanensis Ouattara et al. 2003

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23323negative1.5-2.0 µm0.5 µmrod-shapedyesmonotrichous, polar
120956negativerod-shapedyes

colony morphology

  • @ref: 23323
  • colony shape: circular
  • incubation period: 2-10 days
  • medium used: Trypticase soy

pigmentation

  • @ref: 120956
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4903NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
4903BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
23323Trypticase soyyes
39533MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
120956CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
4903positivegrowth37mesophilic
23323positivegrowth15-42
23323nogrowth4psychrophilic
23323nogrowth45thermophilic
23323positiveoptimum37mesophilic
39533positivegrowth30mesophilic
60723positivegrowth30-37mesophilic
120956positivegrowth25-41
120956nogrowth5psychrophilic
120956nogrowth10psychrophilic

culture pH

@refabilitytypepH
23323positivegrowth5.0-8.0
23323positiveoptimum6.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23323facultative aerobe
60723aerobe
120956obligate aerobe

spore formation

  • @ref: 23323
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
120956NaClpositivegrowth0-4 %
120956NaClnogrowth6 %
120956NaClnogrowth8 %
120956NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23323168082-dehydro-D-gluconate-builds acid from
23323174265-dehydro-D-gluconate-builds acid from
2332327613amygdalin-builds acid from
2332318305arbutin-builds acid from
2332315963ribitol-builds acid from
2332317108D-arabinose-builds acid from
2332318333D-arabitol-builds acid from
2332317057cellobiose-builds acid from
2332315824D-fructose-builds acid from
2332328847D-fucose-builds acid from
2332312936D-galactose-builds acid from
2332317634D-glucose-builds acid from
2332317716lactose-builds acid from
2332362318D-lyxose-builds acid from
2332317306maltose-builds acid from
2332316899D-mannitol-builds acid from
2332316024D-mannose-builds acid from
233236731melezitose-builds acid from
2332328053melibiose-builds acid from
2332316634raffinose-builds acid from
2332316988D-ribose-builds acid from
2332317992sucrose-builds acid from
2332317924D-sorbitol-builds acid from
2332316443D-tagatose-builds acid from
2332316551D-trehalose-builds acid from
2332332528turanose-builds acid from
2332365327D-xylose-builds acid from
2332316813galactitol-builds acid from
2332317113erythritol-builds acid from
233234853esculin-builds acid from
2332328066gentiobiose-builds acid from
2332324265gluconate-builds acid from
2332317754glycerol-builds acid from
2332328087glycogen-builds acid from
2332317268myo-inositol-builds acid from
2332315443inulin-builds acid from
2332330849L-arabinose-builds acid from
2332318403L-arabitol-builds acid from
2332318287L-fucose-builds acid from
2332362345L-rhamnose-builds acid from
2332317266L-sorbose-builds acid from
2332365328L-xylose-builds acid from
23323320061methyl alpha-D-glucopyranoside-builds acid from
2332343943methyl alpha-D-mannoside-builds acid from
2332374863methyl beta-D-xylopyranoside-builds acid from
23323506227N-acetylglucosamine-builds acid from
2332317814salicin-builds acid from
2332328017starch-builds acid from
2332317151xylitol-builds acid from
2332316947citrate-carbon source
23323168082-dehydro-D-gluconate+growth
23323309162-oxoglutarate+growth
23323370543-hydroxybutyrate+growth
2332316865gamma-aminobutyric acid+growth
2332330089acetate+growth
2332335899crotonate+growth
2332318024D-galacturonic acid+growth
233238391D-gluconate+growth
2332315748D-glucuronate+growth
2332315588D-malate+growth
2332333871glycerate+growth
2332324996lactate+growth
2332315740formate+growth
2332317859glutaric acid+growth
2332316977L-alanine+growth
2332316467L-arginine+growth
2332317196L-asparagine+growth
2332317561L-cysteine+growth
2332329985L-glutamate+growth
2332318050L-glutamine+growth
2332315589L-malate+growth
2332317295L-phenylalanine+growth
2332317203L-proline+growth
2332317790methanol+growth
2332315361pyruvate+growth
2332316094thiosulfate+oxidation
12095616947citrate-carbon source
1209564853esculin-hydrolysis
12095617632nitrate-reduction
12095616301nitrite-reduction
12095617632nitrate-respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.group IDis intermediateintermediate conc.is sensitivesensitivity conc.
233233508ceftazidimeyesyes30 µg
23323100241ciprofloxacinyesyes5 µg
233237731ofloxacinyesyes5 µg
233232676amoxicillinyes11yes
233238232piperacillinyesyes75 µg
2332317833gentamicinyesyes10 Unit
2332337943colistinyesyes300 Unit
23323471744imipenemyesyes10 µg
2332328864tobramycinyesyes10 µg
233232637amikacinyesyes30 µg
233233542cephalothinyesyes30 µg
23323204928cefotaximeyesyes30 µg
233237528netilmycinyesyes30 µg
233239587ticarcillinyesyes75 µg
233233770co-trimoxazoleyesyes
233238232piperacillinyes16yes
2332348947clavulanic acidyes11yes
233239421tazobactamyes16yes
1209560129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)yesyesno

metabolite production

@refChebi-IDmetaboliteproduction
2332335581indoleno
12095635581indoleno

metabolite tests

  • @ref: 23323
  • Chebi-ID: 16947
  • metabolite: citrate
  • citrate test: -

enzymes

@refvalueactivityec
23323amylase+/-
23323arginine dihydrolase-3.5.3.6
23323beta-galactosidase-3.2.1.23
23323catalase+1.11.1.6
23323cytochrome oxidase+1.9.3.1
23323DNase-
23323esterase-
23323lysine decarboxylase-4.1.1.18
23323nitrate reductase-1.7.99.4
23323nitrite reductase-1.7.1.4
23323urease+3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
120956oxidase+
120956beta-galactosidase-3.2.1.23
120956alcohol dehydrogenase-1.1.1.1
120956gelatinase+/-
120956amylase+
120956DNase-
120956caseinase-3.4.21.50
120956catalase+1.11.1.6
120956tween esterase-
120956lecithinase-
120956lipase-
120956lysine decarboxylase-4.1.1.18
120956ornithine decarboxylase-4.1.1.17
120956protease-
120956tryptophan deaminase-
120956urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120956-+-+-++-+-++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120956------------------------------------------------------+++---+--------------+--++-++-----+-++-+----+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
4903industrial waste waterIztapalapaMexicoMEXNorth America
23323anaerobic sludge of a lab-scale UASB reactor treating the petrochemical wastewater of a purified terephthalic acid plant
60723Industrial waist waterMexicoMEXNorth America
120956Environment, MudMexicoMEXNorth America1999

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Anaerobic digestor
#Engineered#Waste#Wastewater
#Environmental#Terrestrial#Mud (Sludge)
#Condition#Anoxic (anaerobic)
#Engineered#Waste#Industrial wastewater

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
49031Risk group (German classification)
1209561Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4903
  • description: Bosea minatitlanensis 16S ribosomal RNA gene, partial sequence
  • accession: AF273081
  • length: 1482
  • database: ena
  • NCBI tax ID: 128782

GC content

  • @ref: 4903
  • GC-content: 68.5

External links

@ref: 4903

culture collection no.: CCUG 55772, DSM 13099, ATCC 700918, CIP 106457, AMX 51

straininfo link

  • @ref: 71434
  • straininfo: 44209

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130002Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester.Ouattara AS, Assih EA, Thierry S, Cayol JL, Labat M, Monroy O, Macarie HInt J Syst Evol Microbiol10.1099/ijs.0.02540-02003Aerobiosis, Bradyrhizobiaceae/classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Genes, Bacterial, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sewage/microbiology, Species SpecificityGenetics
Phylogeny24277862Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant.Toth EM, Vengring A, Homonnay ZG, Keki Z, Sproer C, Borsodi AK, Marialigeti K, Schumann PInt J Syst Evol Microbiol10.1099/ijs.0.053843-02013Alphaproteobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hungary, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, *Power Plants, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry, *Water Microbiology, *Water PurificationEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4903Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13099)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13099
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23323Aboubakar S. Ouattara, Essokazi A. Assih, Sébastien Thierry, Jean-Luc Cayol, Marc Labat, Oscar Monroy, Hervé Macarie10.1099/ijs.0.02540-0Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digesterIJSEM 53: 1247-1251 200313130002
39533Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18590
60723Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 55772)https://www.ccug.se/strain?id=55772
68382Automatically annotated from API zym
71434Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44209.1StrainInfo: A central database for resolving microbial strain identifiers
120956Curators of the CIPCollection of Institut Pasteur (CIP 106457)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106457