Strain identifier

BacDive ID: 1786

Type strain: Yes

Species: Bosea thiooxidans

Strain Designation: BI-42

Strain history: <- S. K. Das and A. K. Mishra, Bose Institute, strain BI-42

NCBI tax ID(s): 53254 (species)

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General

@ref: 3671

BacDive-ID: 1786

DSM-Number: 9653

keywords: genome sequence, 16S sequence, Bacteria, facultative aerobe, chemolithoheterotroph, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Bosea thiooxidans BI-42 is a facultative aerobe, chemolithoheterotroph, mesophilic bacterium that forms circular colonies and was isolated from agricultural soil.

NCBI tax id

  • NCBI tax id: 53254
  • Matching level: species

strain history

  • @ref: 3671
  • history: <- S. K. Das and A. K. Mishra, Bose Institute, strain BI-42

doi: 10.13145/bacdive1786.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Boseaceae
  • genus: Bosea
  • species: Bosea thiooxidans
  • full scientific name: Bosea thiooxidans Das et al. 1996

@ref: 3671

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Boseaceae

genus: Bosea

species: Bosea thiooxidans

full scientific name: Bosea thiooxidans Das et al. 1996

strain designation: BI-42

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
23322negative1.4-1.6 µm0.85 µmrod-shapedyesmonotrichous, polar
69480yes96.637
69480negative99.999

colony morphology

  • @ref: 23322
  • colony size: 1.0-1.5 mm
  • colony color: creamy colored
  • colony shape: circular
  • incubation period: 4 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3671TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)yeshttps://mediadive.dsmz.de/medium/545Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
3671BOSEA THIOOXIDANS MEDIUM (DSMZ Medium 763)yeshttps://mediadive.dsmz.de/medium/763Name: BOSEA THIOOXIDANS MEDIUM (DSMZ Medium 763) Composition: Na2S2O3 x 5 H2O 5.0 g/l Sodium succinate 5.0 g/l Na2HPO4 4.0 g/l KH2PO4 1.5 g/l Na glutamate 0.5 g/l Yeast extract 0.1 g/l MgCl2 0.1 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
3671positivegrowth28mesophilic
3671positivegrowth30mesophilic
23322nogrowth<20psychrophilic
23322nogrowth>37mesophilic
23322positiveoptimum30-32mesophilic
60722positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
23322positiveminimum6.0
23322positivemaximum9.0alkaliphile
23322positiveoptimum7.5-8.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23322facultative aerobe
60722aerobe

nutrition type

  • @ref: 23322
  • type: chemolithoheterotroph

spore formation

@refspore formationconfidence
23322no
69481no100
69480no99.992

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2332233403elemental sulfur-growth
2332215428glycine-growth
2332224898isoleucine-growth
2332225017leucine-growth
2332216811methionine-growth
2332228044phenylalanine-growth
2332217359sulfite-growth
2332218022thiocyanate-growth
2332227897tryptophan-growth
2332218186tyrosine-growth
2332228938ammonium-nitrogen source
2332217632nitrate-nitrogen source
2332216199urea-nitrogen source
23322178364-aminobenzoate-
2332217968butyrate-
2332215740formate-
2332217754glycerol-
2332236655glyoxylate-
2332224996lactate-
2332229864mannitol-
2332217790methanol-
2332217272propionate-
2332216634raffinose-
2332230762salicylate-
2332216449alanine+carbon source
2332235391aspartate+carbon source
2332215356cysteine+carbon source
2332229987glutamate+carbon source
2332228300glutamine+carbon source
2332217196L-asparagine+carbon source
2332225094lysine+carbon source
2332226271proline+carbon source
2332217822serine+carbon source
2332228757fructose+growth
2332217234glucose+growth
2332216449alanine+nitrogen source
2332229016arginine+nitrogen source
2332235391aspartate+nitrogen source
2332215356cysteine+nitrogen source
2332229987glutamate+nitrogen source
2332228300glutamine+nitrogen source
2332227570histidine+nitrogen source
2332217196L-asparagine+nitrogen source
2332225094lysine+nitrogen source
2332226271proline+nitrogen source
2332217822serine+nitrogen source
2332215226tetrathionate+oxidation
23322yeast extract+required for growth
2332230089acetate+
2332222599arabinose+
2332216947citrate+
2332228260galactose+
2332224265gluconate+
2332225115malate+
2332215361pyruvate+
2332226546rhamnose+
2332233942ribose+
2332227922sorbose+
2332230031succinate+
2332218222xylose+

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
2332228971ampicillinyesyes200 µg/mL
2332227902tetracyclineyesyes10 µg/mL
2332217076streptomycinyesyes120 µg/mL
2332228077rifampicinyesyes15 µg/mL
2332217698chloramphenicolyesyes20 µg/mL
233227507neomycinyesyes40 µg/mL

enzymes

@refvalueactivityec
23322catalase+1.11.1.6
23322cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3671agricultural soilCalcuttaIndiaINDAsia
60722Agricultural soil

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_3859.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_1319;97_1566;98_1905;99_3859&stattab=map
  • Last taxonomy: Bosea
  • 16S sequence: AF508803
  • Sequence Identity:
  • Total samples: 2348
  • soil counts: 598
  • aquatic counts: 673
  • animal counts: 611
  • plant counts: 466

Safety information

risk assessment

  • @ref: 3671
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bosea thiooxidans strain BI-42 16S ribosomal RNA gene, partial sequenceAF5088031447ena53254
23322Gram-negative sodium thiosulfate oxidizer DNA for 16S rRNAX810441377nuccore53254
3671Bosea thiooxidans 16S rRNA gene, strain DSM 9653AJ2507961465ena53254

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bosea thiooxidans DSM 9653GCA_900168195contigncbi53254
66792Bosea thiooxidans strain DSM 965353254.5wgspatric53254
66792Bosea thiooxidans DSM 96532595698224draftimg53254

GC content

  • @ref: 23322
  • GC-content: 68.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.463yes
anaerobicno97.936no
halophileno94.402no
spore-formingno95.035yes
glucose-utilyes90.681yes
thermophileno95.678yes
motileyes91.151no
aerobicyes91.108no
flagellatedyes62.469yes
glucose-fermentno91.745no

External links

@ref: 3671

culture collection no.: DSM 9653, CCUG 55771, ATCC 700366

straininfo link

  • @ref: 71433
  • straininfo: 46230

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8863427Oxidation of thiosulfate by a new bacterium, Bosea thiooxidans (strain BI-42) gen. nov., sp. nov.: analysis of phylogeny based on chemotaxonomy and 16S ribosomal DNA sequencing.Das SK, Mishra AK, Tindall BJ, Rainey FA, Stackebrandt EInt J Syst Bacteriol10.1099/00207713-46-4-9811996Base Sequence, DNA, Ribosomal/chemistry, Fatty Acids/analysis, Gram-Negative Chemolithotrophic Bacteria/*classification/metabolism, Molecular Sequence Data, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Thiosulfates/*metabolismMetabolism
Phylogeny13130002Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester.Ouattara AS, Assih EA, Thierry S, Cayol JL, Labat M, Monroy O, Macarie HInt J Syst Evol Microbiol10.1099/ijs.0.02540-02003Aerobiosis, Bradyrhizobiaceae/classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Genes, Bacterial, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sewage/microbiology, Species SpecificityGenetics
Phylogeny24277862Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant.Toth EM, Vengring A, Homonnay ZG, Keki Z, Sproer C, Borsodi AK, Marialigeti K, Schumann PInt J Syst Evol Microbiol10.1099/ijs.0.053843-02013Alphaproteobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hungary, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, *Power Plants, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry, *Water Microbiology, *Water PurificationEnzymology
Phylogeny26382584Qingshengfania soli gen. nov., sp. nov., a member of the order Rhizobiales isolated from the soil of a pesticide factory.Zhang L, Zhou QX, Song M, Chen XL, Xu XH, Chen K, Li SP, Jiang JDInt J Syst Evol Microbiol10.1099/ijsem.0.0006202015Alphaproteobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis/chemistry, Molecular Sequence Data, *Pesticides, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryGenetics
Enzymology31706173FRET-based fluorescent nanoprobe platform for sorting of active microorganisms by functional properties.Luo Y, Liu F, Li E, Fang Y, Zhao G, Dai X, Li J, Wang B, Xu M, Liao B, Sun GBiosens Bioelectron10.1016/j.bios.2019.1118322019Azo Compounds/*chemistry, Bacillaceae/isolation & purification, Biosensing Techniques/methods, Bradyrhizobiaceae/isolation & purification, Fluorescence Resonance Energy Transfer/*methods, Fluorescent Dyes/*chemistry, Gram-Negative Bacterial Infections/microbiology, Humans, Nanoparticles/chemistry, Rhodamines/*chemistry, Shewanella/*isolation & purification, Silicon Dioxide/chemistryPhylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3671Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9653)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9653
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23322SUBRATA K. DAS, AJIT K. MISHRA, BRIAN J. TINDALL, FRED A. RAINEY, ERKO STACKEBRANDT10.1099/00207713-46-4-981Oxidation of Thiosulfate by a New Bacterium, Bosea thiooxidans. (strain BI-42) gen. nov., sp. nov.: Analysis of Phylogeny Based on Chemotaxonomy and 16S Ribosomal DNA SequencingIJSEM 46: 981-987 19968863427
60722Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 55771)https://www.ccug.se/strain?id=55771
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71433Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46230.1StrainInfo: A central database for resolving microbial strain identifiers