Strain identifier
BacDive ID: 17846
Type strain: 
Species: Thiohalophilus thiocyanatoxydans
Strain history: <- D. Y. Sorokin, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; HRhD 2 (Thiohalophilus thiocyanoxidans) <- D. Y. Sorokin {2003}
NCBI tax ID(s): 381308 (species)
version 8.1 (current version)
General
@ref: 6368
BacDive-ID: 17846
DSM-Number: 16326
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, motile
description: Thiohalophilus thiocyanatoxydans DSM 16326 is a microaerophile, mesophilic, motile bacterium that was isolated from sediments from a hypersaline lake.
NCBI tax id
- NCBI tax id: 381308
- Matching level: species
strain history
- @ref: 6368
- history: <- D. Y. Sorokin, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; HRhD 2 (Thiohalophilus thiocyanoxidans) <- D. Y. Sorokin {2003}
doi: 10.13145/bacdive17846.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Chromatiales
- family: Thioalkalispiraceae
- genus: Thiohalophilus
- species: Thiohalophilus thiocyanatoxydans
- full scientific name: Thiohalophilus thiocyanatoxydans corrig. Sorokin et al. 2007
synonyms
- @ref: 20215
- synonym: Thiohalophilus thiocyanoxidans
@ref: 6368
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Chromatiales
family: Thioalkalispiraceae
genus: Thiohalophilus
species: Thiohalophilus thiocyanatoxydans
full scientific name: Thiohalophilus thiocyanatoxydans Sorokin et al. 2007
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 90.495 | |
| 69480 | 99.998 | negative |
Culture and growth conditions
culture medium
- @ref: 6368
- name: THIOHALOPHILUS MEDIUM (DSMZ Medium 1058)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1058
- composition: Name: THIOHALOPHILUS MEDIUM (DSMZ Medium 1058) Composition: NaCl 119.76 g/l Na2S2O3 x 5 H2O 4.99002 g/l NaHCO3 4.99002 g/l K2HPO4 1.49701 g/l MgSO4 x 7 H2O 0.499002 g/l NH4Cl 0.499002 g/l CaCl2 x 2 H2O 0.0499002 g/l EDTA 0.00499002 g/l FeSO4 x 7 H2O 0.00219561 g/l Pyridoxine hydrochloride 0.000299401 g/l CoCl2 x 6 H2O 0.000199601 g/l Thiamine-HCl x 2 H2O 0.000199601 g/l Nicotinic acid 0.000199601 g/l ZnSO4 x 7 H2O 9.98004e-05 g/l Calcium pantothenate 9.98004e-05 g/l Vitamin B12 9.98004e-05 g/l p-Aminobenzoic acid 7.98403e-05 g/l Na2MoO4 x 2 H2O 2.99401e-05 g/l MnCl2 x 4 H2O 2.99401e-05 g/l H3BO3 2.99401e-05 g/l CuCl2 x 2 H2O 2.99401e-05 g/l NiCl2 x 6 H2O 2.99401e-05 g/l D-(+)-biotin 1.99601e-05 g/l Distilled water
culture temp
- @ref: 6368
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 6368
- oxygen tolerance: microaerophile
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.967 |
Isolation, sampling and environmental information
isolation
- @ref: 6368
- sample type: sediments from a hypersaline lake
- geographic location: South-Western Siberia
- country: Russia
- origin.country: RUS
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Aquatic | #Sediment |
| #Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_73756.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_159;96_26417;97_39687;98_51639;99_73756&stattab=map
- Last taxonomy: Thiohalophilus thiocyanatoxydans subclade
- 16S sequence: DQ469584
- Sequence Identity:
- Total samples: 3
- aquatic counts: 3
Safety information
risk assessment
- @ref: 6368
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6368
- description: Thiohalophilus thiocyanatoxydans strain HRhD 2 16S ribosomal RNA gene, partial sequence
- accession: DQ469584
- length: 1297
- database: ena
- NCBI tax ID: 381308
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thiohalophilus thiocyanatoxydans DSM 16326 | GCA_004366735 | scaffold | ncbi | 381308 |
| 66792 | Thiohalophilus thiocyanatoxydans strain DSM 16326 | 381308.3 | wgs | patric | 381308 |
| 66792 | Thiohalophilus thiocyanatoxydans DSM 16326 | 2786546142 | draft | img | 381308 |
GC content
- @ref: 6368
- GC-content: 58.2
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| flagellated | no | 84.237 | no |
| gram-positive | no | 99.291 | no |
| anaerobic | no | 95.071 | yes |
| aerobic | no | 92.099 | yes |
| halophile | yes | 58.988 | no |
| spore-forming | no | 96.349 | no |
| glucose-util | yes | 57.65 | no |
| thermophile | no | 96.757 | no |
| motile | no | 71.226 | no |
| glucose-ferment | no | 89.879 | no |
External links
@ref: 6368
culture collection no.: DSM 16326, UNIQEM U231, HRhD 2
straininfo link
- @ref: 86837
- straininfo: 400747
literature
- topic: Metabolism
- Pubmed-ID: 17216167
- title: Denitrification in a binary culture and thiocyanate metabolism in Thiohalophilus thiocyanoxidans gen. nov. sp. nov. - a moderately halophilic chemolithoautotrophic sulfur-oxidizing Gammaproteobacterium from hypersaline lakes.
- authors: Sorokin DY, Tourova TP, Bezsoudnova EY, Pol A, Muyzer G
- journal: Arch Microbiol
- DOI: 10.1007/s00203-006-0208-3
- year: 2007
- mesh: Aerobiosis, Anaerobiosis, *Chemoautotrophic Growth, Culture Media, Fresh Water/chemistry/*microbiology, *Gammaproteobacteria/classification/genetics/growth & development/metabolism, Geologic Sediments/microbiology, Molecular Sequence Data, Nitrites/*metabolism, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/*pharmacology, Sulfur/*metabolism, Thiocyanates/*metabolism
- topic2: Phylogeny
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 6368 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16326) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16326 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 86837 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID400747.1 | StrainInfo: A central database for resolving microbial strain identifiers |