Strain identifier

BacDive ID: 17827

Type strain: Yes

Species: Sutterella wadsworthensis

Strain history: CIP <- 1996, S. Finegold, VA Wadsworth, Los Angeles, CA, USA: strain WAL 9799 <- VA Wadsworth

NCBI tax ID(s): 40545 (species)

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General

@ref: 5221

BacDive-ID: 17827

DSM-Number: 14016

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, rod-shaped

description: Sutterella wadsworthensis DSM 14016 is an anaerobe, mesophilic, rod-shaped bacterium that was isolated from human abdominal fluid.

NCBI tax id

  • NCBI tax id: 40545
  • Matching level: species

strain history

@refhistory
5221<- ATCC <- S. M. Finegold; WAL 9799
67770CCUG 42229 <-- CIP 104799 <-- S. M. Finegold; WAL 9799 <-- C. A. Wadsworth.
67771<- DSM <- ATCC <- SM Finegold, WAL 9799
123900CIP <- 1996, S. Finegold, VA Wadsworth, Los Angeles, CA, USA: strain WAL 9799 <- VA Wadsworth

doi: 10.13145/bacdive17827.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Sutterellaceae
  • genus: Sutterella
  • species: Sutterella wadsworthensis
  • full scientific name: Sutterella wadsworthensis Wexler et al. 1996

@ref: 5221

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Sutterellaceae

genus: Sutterella

species: Sutterella wadsworthensis

full scientific name: Sutterella wadsworthensis Wexler et al. 1996

type strain: yes

Morphology

cell morphology

@refcell shapegram stainmotility
68367rod-shaped
68367negative
67771rod-shaped
67771negative
123900rod-shapednegativeno

colony morphology

@refincubation period
52211-2 days
123900

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5221BTU MEDIUM (DSMZ Medium 413)yeshttps://mediadive.dsmz.de/medium/413Name: BTU MEDIUM (DSMZ Medium 413) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l Na-formiate 1.8 g/l Na-fumarate 1.8 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Resazurin 0.001 g/l Vitamin K1 NaOH Distilled water
35734MEDIUM 45 - for Columbia agar with sheep bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
123900CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45

culture temp

@refgrowthtypetemperaturerange
5221positivegrowth37mesophilic
35734positivegrowth37mesophilic
55368positivegrowth37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5221anaerobe
55368anaerobe
67771anaerobe
123900facultative anaerobe

spore formation

  • @ref: 68367
  • spore formation: no

halophily

  • @ref: 123900
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 3.5 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68367trehalose-builds acid from27082
68367L-rhamnose-builds acid from62345
68367sorbitol-builds acid from30911
68367raffinose-builds acid from16634
68367melezitose-builds acid from6731
68367D-mannose-builds acid from16024
68367cellobiose-builds acid from17057
68367glycerol-builds acid from17754
68367esculin-hydrolysis4853
68367gelatin-hydrolysis5291
68367L-arabinose-builds acid from30849
68367D-xylose-builds acid from65327
68367salicin-builds acid from17814
68367maltose-builds acid from17306
68367sucrose-builds acid from17992
68367lactose-builds acid from17716
68367D-mannitol-builds acid from16899
68367D-glucose-builds acid from17634
68367urea-hydrolysis16199
68367tryptophan-energy source27897
123900D-arabinose-degradation17108
123900D-fructose-degradation15824
123900D-glucose-degradation17634
123900D-xylose-degradation65327
123900cellobiose+degradation17057
123900lactose-degradation17716
123900maltose-degradation17306
123900salicin-degradation17814
123900sucrose-degradation17992
123900citrate-carbon source16947
123900esculin-hydrolysis4853
123900nitrate+reduction17632
123900nitrite-reduction16301
68380urea-hydrolysis16199
68380D-mannose-fermentation16024
68380raffinose-fermentation16634
68380L-glutamate-degradation29985
68380nitrate-reduction17632
68380tryptophan-energy source27897

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
1239006909metronidazoleyesyesno
1239000129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)yesnoyes

metabolite production

@refChebi-IDmetaboliteproduction
6836735581indoleno
12390035581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6838035581indole-
6836735581indole-
12390015688acetoin-

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380urease-3.5.1.5
68367catalase-1.11.1.6
68367beta-glucosidase-3.2.1.21
68367gelatinase-
68367urease-3.5.1.5
123900oxidase-
123900beta-galactosidase-3.2.1.23
123900alcohol dehydrogenase-1.1.1.1
123900gelatinase-
123900amylase-
123900DNase+
123900caseinase-3.4.21.50
123900catalase-1.11.1.6
123900tween esterase-
123900gamma-glutamyltransferase-2.3.2.2
123900lecithinase-
123900lipase-
123900lysine decarboxylase-4.1.1.18
123900ornithine decarboxylase-4.1.1.17
123900protease-
123900urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123900--++------+---------

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRECATSPORGRAMCOCC
5221------------------------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123900----+/----+/----------+/--------------------+/-+--+/-------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
5221-----------------------------
5221-+/----------------+/----+/--+/-+/-+/----
5221------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic locationisolation date
5221human abdominal fluidUSAUSANorth America
55368Human abdominal fluidUSAUSANorth America1991CA,Los Angeles
67770Abdominal fluidUSAUSANorth America
67771From human abdominal fluid, gastrointestinal tractsUSAUSANorth America
123900Human, Abdominal fluidUnited States of AmericaUSANorth AmericaLos Angeles, California1991

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Other#Abdomen
#Host Body Product#Fluids
#Infection#Patient

taxonmaps

  • @ref: 69479
  • File name: preview.99_2536.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_173;96_1365;97_1621;98_1972;99_2536&stattab=map
  • Last taxonomy: Sutterella wadsworthensis subclade
  • 16S sequence: HM037997
  • Sequence Identity:
  • Total samples: 143308
  • soil counts: 1088
  • aquatic counts: 4769
  • animal counts: 137097
  • plant counts: 354

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
52212Risk group (German classification)
1239001Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Sutterella wadsworthensis culture-collection ATCC:51579 16S ribosomal RNA gene, partial sequenceGU5856691449ena40545
5221Sutterella wadsworthensis ATCC:51579 16S ribosomal RNA gene, partial sequenceHM0379971462ena40545

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sutterella wadsworthensis DSM 14016GCA_003315195scaffoldncbi40545
66792Sutterella wadsworthensis strain DSM 1401640545.5wgspatric40545
66792Sutterella wadsworthensis strain FDAARGOS_115940545.1270completepatric40545
66792Sutterella wadsworthensis DSM 140162770939567draftimg40545

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno97.846yes
anaerobicyes97.15yes
halophileno64.145no
spore-formingno97.292no
glucose-utilno65.152no
aerobicno97.741yes
flagellatedno94.629no
motileno91.616no
thermophileno99.475yes
glucose-fermentno76.249no

External links

@ref: 5221

culture collection no.: DSM 14016, ATCC 51579, CIP 104799, KCTC 15691, CCUG 42229, WAL 9799, JCM 32440

straininfo link

  • @ref: 86818
  • straininfo: 47039

literature

  • topic: Phylogeny
  • Pubmed-ID: 8573504
  • title: Sutterella wadsworthensis gen. nov., sp. nov., bile-resistant microaerophilic Campylobacter gracilis-like clinical isolates.
  • authors: Wexler HM, Reeves D, Summanen PH, Molitoris E, McTeague M, Duncan J, Wilson KH, Finegold SM
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-46-1-252
  • year: 1996
  • mesh: Base Sequence, *Bile, Campylobacter/classification, DNA, Bacterial/genetics, Drug Resistance, Microbial, Fatty Acids/analysis, Gram-Negative Bacteria/*classification/genetics/metabolism, Humans, Metronidazole/pharmacology, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidoreductases/metabolism, Oxygen/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Tetrazolium Salts/metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5221Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14016)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14016
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35734Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16747
55368Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 42229)https://www.ccug.se/strain?id=42229
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68367Automatically annotated from API 20A
68371Automatically annotated from API 50CH acid
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86818Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47039.1StrainInfo: A central database for resolving microbial strain identifiers
123900Curators of the CIPCollection of Institut Pasteur (CIP 104799)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104799