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Strain identifier

BacDive ID: 17823

Type strain:

Species: Jiangella alba

Culture col. no.: DSM 45237, CCTCC AA 208023, JCM 16901, YIM 61503

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 15906

BacDive-ID: 17823

DSM-Number: 45237

keywords: aerobe, Bacteria, 16S sequence, genome sequence, mesophilic, gram-positive

description: Jiangella alba DSM 45237 is an aerobe, mesophilic, gram-positive bacterium that builds an aerial mycelium and was isolated from surface-sterilized healthy stem of the medicinal plant Maytenus austroyunnanensis.

strain history: <- S.-K. Tang, YIM; YIM 61503 <- S. Qin and et al.

doi: 10.13145/bacdive17823.20201210.5

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/actinobacteria
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Jiangellales
family: Jiangellaceae
genus: Jiangella
species: Jiangella alba
full scientific name: Jiangella alba Qin et al. 2009

@ref: 15906

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Jiangellaceae

genus: Jiangella

species: Jiangella alba

full scientific name: Jiangella alba Qin et al. 2009

type strain: yes

Morphology

cell morphology

@ref: 29229
gram stain: positive

colony morphology

@ref	colony color	incubation period	medium used
20186	Beige (1001)	10-14 days	ISP 2
20186	Grey white (9002)	10-14 days	ISP 3
20186	Signal white (9003)	10-14 days	ISP 4
20186	Beige (1001)	10-14 days	ISP 6
20186	Beige (1001)	10-14 days	ISP 7

multicellular morphology

@ref	forms multicellular complex	medium name	complex name	complex color
20186	no	ISP 2
20186	yes	ISP 3	Aerial Mycelium	Grey white
20186	no	ISP 4
20186	yes	ISP 6	Aerial Mycelium	Light ivory
20186	no	ISP 7

multimedia

@ref: 15906
multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45237.jpg
caption: Medium 215 28°C
license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
15906	BHI MEDIUM (DSMZ Medium 215)	yes	https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215.pdf
20186	ISP 2	yes		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20186	ISP 3	yes		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20186	ISP 4	yes		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20186	ISP 6	yes		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20186	ISP 7	yes		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@ref	growth	type	temperature	range
15906	positive	growth	28	mesophilic
20186	positive	optimum	28	mesophilic
29229	positive	growth	15-45
29229	positive	optimum	28	mesophilic

culture pH

@ref	ability	type	pH
29229	positive	growth	6.5-9
29229	positive	optimum	7

Physiology and metabolism

oxygen tolerance

@ref: 29229
oxygen tolerance: aerobe

halophily

@ref	salt	growth	tested relation	concentration
29229	NaCl	positive	growth	0-10 %
29229	NaCl	positive	optimum	5 %

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
20186	17234	glucose	+
20186	22599	arabinose	+
20186	17992	sucrose	+
20186	18222	xylose	+
20186	17268	myo-inositol	-
20186	29864	mannitol	-
20186	28757	fructose	-
20186	26546	rhamnose	+
20186	16634	raffinose	+
20186	62968	cellulose	+
29229	22599	arabinose	+	carbon source
29229	28757	fructose	+	carbon source
29229	17234	glucose	+	carbon source
29229	17754	glycerol	+	carbon source
29229	17306	maltose	+	carbon source
29229	29864	mannitol	+	carbon source
29229	37684	mannose	+	carbon source
29229	16634	raffinose	+	carbon source
29229	26546	rhamnose	+	carbon source
29229	33942	ribose	+	carbon source
29229	17814	salicin	+	carbon source
29229	17992	sucrose	+	carbon source
29229	27082	trehalose	+	carbon source
29229	18222	xylose	+	carbon source
29229	17632	nitrate	+	reduction

enzymes

@ref: 29229
value: catalase
activity: +
ec: 1.11.1.6

API coryne

@ref	NIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG
20186	-	-	+	+	-	+	+	-	-	-	+	+/-	-	-	-	-	-	+	-

API zym

@ref	Alkaline phosphatase	Esterase	Esterase Lipase	Lipase	Leucine arylamidase	Valine arylamidase	Cystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20186	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	-	-	+	-

Isolation, sampling and environmental information

isolation

@ref: 15906
sample type: surface-sterilized healthy stem of the medicinal plant Maytenus austroyunnanensis
host species: Maytenus austroyunnanensis
geographic location: Yunnan province, Xishuangbanna, tropical rainforest
country: China
origin.country: CHN
continent: Asia

isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Stem (Branch)
#Host Body-Site	#Plant	#Sterilized plant part

Safety information

risk assessment

@ref: 15906
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 15906
description: Jiangella alba strain YIM 61503 16S ribosomal RNA gene, partial sequence
accession: FJ157186
length: 1519
database: ena
NCBI tax ID: 561176

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Jiangella alba DSM 45237	GCA_900106035	contig	ncbi	561176
66792	Jiangella alba YIM 61503	GCA_001708125	scaffold	ncbi	561176
66792	Jiangella alba strain DSM 45237	561176.5	wgs	patric	561176
66792	Jiangella alba strain YIM 61503	561176.4	wgs	patric	561176
66792	Jiangella alba DSM 45237	2630968302	permanent	img	561176

GC content

@ref	GC-content	method
15906	71.9	high performance liquid chromatography (HPLC)
29229	71.9

External links

@ref: 15906

culture collection no.: DSM 45237, CCTCC AA 208023, JCM 16901, YIM 61503

straininfo link

@ref	passport
20218	http://www.straininfo.net/strains/844588
20218	http://www.straininfo.net/strains/844587
20218	http://www.straininfo.net/strains/856469
20218	http://www.straininfo.net/strains/844586

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed	ID_cross_reference
15906	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45237)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-45237
20186	Wink, J.		http://www.dsmz.de/microorganisms/wink_pdf/DSM45237.pdf	Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215	D.Gleim, M.Kracht, N.Weiss et. al.		http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html	Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
25650			10.1099/ijs.0.009001-0
29229	Barberan A, Caceres Velazquez H, Jones S, Fierer N.		10.1128/mSphere.00237-17	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information			25650
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)