Strain identifier

BacDive ID: 17822

Type strain: Yes

Species: Jiangella alkaliphila

Strain Designation: D8-87

Culture col. no.: DSM 45079, JBRI 2008, KCTC 19222

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General

@ref: 12322

BacDive-ID: 17822

DSM-Number: 45079

keywords: aerobe, Bacteria, 16S sequence, genome sequence, mesophilic, gram-positive

description: Jiangella alkaliphila D8-87 is an aerobe, mesophilic, gram-positive bacterium that builds an aerial mycelium and was isolated from soil inside cave.

strain history: <- S. D. Lee; D8-87

doi: 10.13145/bacdive17822.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Jiangellales
  • family: Jiangellaceae
  • genus: Jiangella
  • species: Jiangella alkaliphila
  • full scientific name: Jiangella alkaliphila Lee 2008

@ref: 12322

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Jiangellaceae

genus: Jiangella

species: Jiangella alkaliphila

full scientific name: Jiangella alkaliphila Lee 2008

strain designation: D8-87

type strain: yes

Morphology

cell morphology

  • @ref: 32426
  • gram stain: positive

colony morphology

@refcolony colorincubation periodmedium used
19912Beige10-14 daysISP 2
19912Cream10-14 daysISP 3
19912Beige10-14 daysISP 4
1991210-14 daysISP 5
1991210-14 daysISP 6
1991210-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium namecomplex namecomplex color
19912noISP 2
19912yesISP 3Aerial MyceliumCream
19912yesISP 4Aerial MyceliumCream
19912noISP 5
19912noISP 6
19912noISP 7

multimedia

  • @ref: 12322
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45079.jpg
  • caption: Medium 65 28°C
  • license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • intellectual property rights: © Leibniz-Institut DSMZ

pigmentation

  • @ref: 32426
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12322GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
19912ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19912ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19912ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19912ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19912ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19912ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
12322positivegrowth28mesophilic
19912positiveoptimum28mesophilic
32426positivegrowth20-37
32426positiveoptimum30mesophilic

culture pH

@refabilitytypepH
32426positivegrowth6.1-10.1
32426positiveoptimum9.1

Physiology and metabolism

oxygen tolerance

  • @ref: 32426
  • oxygen tolerance: aerobe

halophily

  • @ref: 32426
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <3 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3242615963ribitol+carbon source
3242622599arabinose+carbon source
3242617057cellobiose+carbon source
3242628757fructose+carbon source
3242617234glucose+carbon source
3242617754glycerol+carbon source
3242617716lactose+carbon source
3242617306maltose+carbon source
3242629864mannitol+carbon source
3242637684mannose+carbon source
3242626546rhamnose+carbon source
3242618222xylose+carbon source
324264853esculin+hydrolysis

enzymes

@refvalueactivityec
32426catalase+1.11.1.6
32426gelatinase+

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19912++-------+++---+-+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19912+++----+-++---+----

Isolation, sampling and environmental information

isolation

  • @ref: 12322
  • sample type: soil inside cave
  • geographic location: Jeju
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Geologic
#Environmental#Terrestrial#Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
123221Risk group (German classification)
199121Risk group (German classification)

Sequence information

16S sequences

  • @ref: 12322
  • description: Jiangella alkaliphila partial 16S rRNA gene, type strain D8-87T
  • accession: AM422451
  • length: 1406
  • database: ena
  • NCBI tax ID: 419479

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Jiangella alkaliphila DSM 45079GCA_900105925chromosomencbi419479
66792Jiangella alkaliphila KCTC 19222GCA_001005145scaffoldncbi419479
66792Jiangella alkaliphila strain DSM 45079419479.6completepatric419479
66792Jiangella alkaliphila strain KCTC 19222419479.7wgspatric419479
66792Jiangella alkaliphila DSM 450792639762615permanentimg419479

GC content

  • @ref: 12322
  • GC-content: 71.5

External links

@ref: 12322

culture collection no.: DSM 45079, JBRI 2008, KCTC 19222

straininfo link

@refpassport
20218http://www.straininfo.net/strains/822638
20218http://www.straininfo.net/strains/822639
20218http://www.straininfo.net/strains/822640

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
12322Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45079)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45079
19912Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45079.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2864910.1099/ijs.0.65479-0
32426Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information28649
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)