Strain identifier

BacDive ID: 17802

Type strain: Yes

Species: Ruania alba

Strain history: <- SK Tang, Yunnan Inst., China

NCBI tax ID(s): 648782 (species)

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General

@ref: 15529

BacDive-ID: 17802

DSM-Number: 21368

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Ruania alba DSM 21368 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil from a salt lake.

NCBI tax id

  • NCBI tax id: 648782
  • Matching level: species

strain history

@refhistory
15529<- S.-K. Tang, YIM; YIM 93306
67770W.-J. Li YIM 93306.
67771<- SK Tang, Yunnan Inst., China

doi: 10.13145/bacdive17802.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Ruaniaceae
  • genus: Ruania
  • species: Ruania alba
  • full scientific name: Ruania alba (Tang et al. 2010) Schumann et al. 2021

  • synonyms

    • @ref: 20215
    • synonym: Haloactinobacterium album

@ref: 15529

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Ruaniaceae

genus: Ruania

species: Ruania alba

full scientific name: Ruania alba (Tang et al. 2010) Schumann et al. 2021

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
67771positive
69480no94.841
69480positive100

colony morphology

@refcolony colorincubation periodmedium used
19794Ivory10-14 daysISP 2
19794Ivory10-14 daysISP 3
19794Light ivory10-14 daysISP 4
19794Light ivory10-14 daysISP 5
19794Ivory10-14 daysISP 6
19794Light ivory10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19794noISP 2
19794noISP 3
19794noISP 4
19794noISP 5
19794noISP 6
19794noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15529TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
19794ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19794ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19794ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19794ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19794ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19794ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
15529positivegrowth28mesophilic
19794positiveoptimum28mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 67771
  • oxygen tolerance: aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.991

murein

  • @ref: 15529
  • murein short key: A11.54
  • type: A4alpha L-Lys-L-Glu

observation

  • @ref: 67770
  • observation: quinones: MK-8(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activity
1979462968cellulose-
1979416634raffinose+
1979426546rhamnose-
1979428757fructose-
1979429864mannitol-
1979417268myo-inositol-
1979418222xylose-
1979417992sucrose+
1979422599arabinose-
1979417234glucose-

enzymes

@refvalueactivityec
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase+3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19794--+-+---+-+++-+++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
15529soil from a salt lakeXinjiang province, Qijiaojing Lake, 43°26'48''N 91°29'13''EChinaCHNAsia43.446791.4869
67770Soil from a salt lakeXinjiang Province, north-west ChinaChinaCHNAsia
67771From salt lakeXinjiang ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Terrestrial#Soil
#Condition#Saline

Safety information

risk assessment

  • @ref: 15529
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15529
  • description: Haloactinobacterium album strain YIM 93306 16S ribosomal RNA gene, partial sequence
  • accession: FJ969847
  • length: 1524
  • database: ena
  • NCBI tax ID: 648782

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Haloactinobacterium album strain DSM 21368648782.3wgspatric648782
66792Ruania alba DSM 213682634166281draftimg648782
67770Ruania alba DSM 21368GCA_900105765contigncbi648782

GC content

@refGC-contentmethod
1552968.3high performance liquid chromatography (HPLC)
6777068.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno68no
gram-positiveyes87.535yes
anaerobicno99.175yes
halophileno75.033no
spore-formingno92.416no
glucose-utilyes79.849yes
thermophileno98.315yes
flagellatedno98.627no
aerobicyes90.118yes
motileno91.12no
glucose-fermentno87.185no

External links

@ref: 15529

culture collection no.: DSM 21368, CCTCC AB 208069, KCTC 19413, YIM 93306, JCM 19145, NBRC 109067

straininfo link

  • @ref: 86794
  • straininfo: 400106

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19854874Haloactinobacterium album gen. nov., sp. nov., a halophilic actinobacterium, and proposal of Ruaniaceae fam. nov.Tang SK, Zhi XY, Wang Y, Wu JY, Lee JC, Kim CJ, Lou K, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.018440-02009Actinobacteria/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Fresh Water/microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolismMetabolism
Phylogeny31483241Haloactinobacterium glacieicola sp. nov., isolated from an ice core.Wang F, Liu Y, Wang N, Xu B, Shen L, Gu Z, Liu K, Liu H, Zhou YInt J Syst Evol Microbiol10.1099/ijsem.0.0036532019Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Ice Cover/*microbiology, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny34164698Ruania alkalisoli sp. nov., Isolated from Saline-Alkaline Soil.Sun JQ, Yiayeng PE, Xu L, Huang XX, Li YCurr Microbiol10.1007/s00284-021-02579-62021Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil, *Soil MicrobiologyTranscriptome
Phylogeny34388085Identification of Haloactinobacterium kanbiaonis sp. nov. and Ruania zhangjianzhongii sp. nov., two novel species of the family Ruaniaceae isolated from faeces of bats (Hipposideros spp.).Xu M, Dai Y, Huang Y, Yang J, Lai XH, Jin D, Lu S, Zhou J, Zhang S, Bai Y, Jiao Y, Qiao L, Jiang Y, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0049532021Actinobacteria/*classification/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, *Chiroptera/microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny34542394Ruania rhizosphaerae sp. nov., a novel actinobacterium isolated from rhizosphere of Suaeda aralocaspica.Lu WN, Xu YZ, Xie YG, Gao R, Song JQ, Liu DF, Xiao M, Wang HF, Li QL, Li WJInt J Syst Evol Microbiol10.1099/ijsem.0.0050072021Actinobacteria, Bacterial Typing Techniques, Base Composition, *Chenopodiaceae, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, Soil MicrobiologyTranscriptome
Phylogeny35763330Ruania suaedae sp. nov. and Ruania halotolerans sp. nov., two actinobacteria isolated from saline soil, and reclassification of Haloactinobacterium kanbiaonis as Occultella kanbiaonis comb. nov.Xu L, Shang J, Tian J, Sun JQ, Shen BInt J Syst Evol Microbiol10.1099/ijsem.0.0054432022*Actinobacteria, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, SoilTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15529Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21368)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21368
19794Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM21368.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
86794Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID400106.1StrainInfo: A central database for resolving microbial strain identifiers