Strain identifier
BacDive ID: 17800
Type strain: 
Species: Acidilobus saccharovorans
Strain Designation: 345-15
Strain history: DSM 16705 <-- M. Prokofeva 345-15.
NCBI tax ID(s): 242703 (species)
version 8.1 (current version)
General
@ref: 6576
BacDive-ID: 17800
DSM-Number: 16705
keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic
description: Acidilobus saccharovorans 345-15 is an anaerobe, hyperthermophilic archaeon that was isolated from mixture of water and mud from an acidic terrestrial hot pool.
NCBI tax id
- NCBI tax id: 242703
- Matching level: species
strain history
| @ref | history |
|---|---|
| 6576 | <- M. Prokofeva, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; 345-15 <- M. Prokofeva {1999} |
| 67770 | DSM 16705 <-- M. Prokofeva 345-15. |
doi: 10.13145/bacdive17800.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/thermoproteota
- domain: Archaea
- phylum: Thermoproteota
- class: Thermoprotei
- order: Acidilobales
- family: Acidilobaceae
- genus: Acidilobus
- species: Acidilobus saccharovorans
- full scientific name: Acidilobus saccharovorans Prokofeva et al. 2009
@ref: 6576
domain: Archaea
phylum: Crenarchaeota
class: Thermoprotei
order: Acidilobales
family: Acidilobaceae
genus: Acidilobus
species: Acidilobus saccharovorans
full scientific name: Acidilobus saccharovorans Prokofeva et al. 2009
strain designation: 345-15
type strain: yes
Morphology
colony morphology
- @ref: 6576
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 6576
- name: ACIDOLOBUS ACETICUS MEDIUM (DSMZ Medium 901)
- growth: yes
- link: https://mediadive.dsmz.de/medium/901
- composition: Name: ACIDOLOBUS ACETICUS MEDIUM (DSMZ Medium 901; with strain-specific modifications) Composition: Sulfur 9.83284 g/l D-Glucose 1.96657 g/l Na2S x 9 H2O 0.442478 g/l CaCl2 x 2 H2O 0.324484 g/l MgCl2 x 6 H2O 0.324484 g/l KH2PO4 0.324484 g/l NH4Cl 0.324484 g/l KCl 0.324484 g/l Yeast extract 0.0983284 g/l HCl 0.00245821 g/l FeCl2 x 4 H2O 0.00147493 g/l Sodium resazurin 0.000491642 g/l CoCl2 x 6 H2O 0.000186824 g/l Pyridoxine hydrochloride 9.83284e-05 g/l MnCl2 x 4 H2O 9.83284e-05 g/l ZnCl2 6.88299e-05 g/l p-Aminobenzoic acid 4.91642e-05 g/l Calcium D-(+)-pantothenate 4.91642e-05 g/l Nicotinic acid 4.91642e-05 g/l Riboflavin 4.91642e-05 g/l (DL)-alpha-Lipoic acid 4.91642e-05 g/l Thiamine HCl 4.91642e-05 g/l Na2MoO4 x 2 H2O 3.53982e-05 g/l NiCl2 x 6 H2O 2.35988e-05 g/l Biotin 1.96657e-05 g/l Folic acid 1.96657e-05 g/l H3BO3 5.89971e-06 g/l CuCl2 x 2 H2O 1.96657e-06 g/l Vitamin B12 9.83284e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 6576 | positive | growth | 80 | hyperthermophilic |
| 67770 | positive | growth | 80 | hyperthermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 6576
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 6576 | mixture of water and mud from an acidic terrestrial hot pool | Kamchatka Peninsula, Orange Thermal Field, 54° 30' 40'' N 160° 00' 06'' E | Russia | RUS | Asia | 54.5111 | 160.002 |
| 67770 | Hot acidic pool in the Orange Thermal Field of Uzon Caldera | Kamchatka | Russia | RUS |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | |
| #Environmental | #Terrestrial | #Mud (Sludge) |
| #Condition | #Acidic | |
| #Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_192351.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17848;96_68850;97_92249;98_125381;99_192351&stattab=map
- Last taxonomy: Acidilobus
- 16S sequence: AY350586
- Sequence Identity:
- Total samples: 5
- aquatic counts: 5
Safety information
risk assessment
- @ref: 6576
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 6576
- description: Acidilobus saccharovorans strain 345-15 16S ribosomal RNA gene, partial sequence
- accession: AY350586
- length: 1451
- database: ena
- NCBI tax ID: 666510
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Acidilobus saccharovorans 345-15 | GCA_000144915 | complete | ncbi | 666510 |
| 66792 | Acidilobus saccharovorans 345-15 | 648028003 | complete | img | 666510 |
| 66792 | Acidilobus saccharovorans 345-15 | 666510.18 | complete | patric | 666510 |
GC content
- @ref: 6576
- GC-content: 54.5
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 83.229 | no |
| flagellated | no | 97.078 | no |
| gram-positive | no | 96.087 | no |
| anaerobic | yes | 97.267 | yes |
| aerobic | no | 94.759 | yes |
| halophile | no | 83.844 | no |
| spore-forming | no | 96.212 | no |
| thermophile | yes | 100 | no |
| glucose-util | yes | 87.546 | no |
| glucose-ferment | no | 63.801 | no |
External links
@ref: 6576
culture collection no.: DSM 16705, JCM 18335, VKM B-2471
straininfo link
- @ref: 86792
- straininfo: 405657
literature
- topic: Phylogeny
- Pubmed-ID: 19643887
- title: Isolation of the anaerobic thermoacidophilic crenarchaeote Acidilobus saccharovorans sp. nov. and proposal of Acidilobales ord. nov., including Acidilobaceae fam. nov. and Caldisphaeraceae fam. nov.
- authors: Prokofeva MI, Kostrikina NA, Kolganova TV, Tourova TP, Lysenko AM, Lebedinsky AV, Bonch-Osmolovskaya EA
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.010355-0
- year: 2009
- mesh: Anaerobiosis, Crenarchaeota/*classification/genetics/*isolation & purification, DNA, Archaeal/genetics, DNA, Ribosomal/genetics, Hot Springs/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 6576 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16705) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16705 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 86792 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID405657.1 | StrainInfo: A central database for resolving microbial strain identifiers |