Strain identifier
BacDive ID: 17797
Type strain: 
Species: Salisaeta longa
Strain Designation: S4-4
Strain history: <- A. Oren, Hebrew Univ. Jerusalem, Israel; S4-4
NCBI tax ID(s): 1089550 (strain), 503170 (species)
version 8.1 (current version)
General
@ref: 15558
BacDive-ID: 17797
DSM-Number: 21114
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped
description: Salisaeta longa S4-4 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from experimental mesocosm filled with a mixture of water from the Dead Sea and the Red Sea.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1089550 | strain |
| 503170 | species |
strain history
- @ref: 15558
- history: <- A. Oren, Hebrew Univ. Jerusalem, Israel; S4-4
doi: 10.13145/bacdive17797.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/rhodothermota
- domain: Bacteria
- phylum: Rhodothermota
- class: Rhodothermia
- order: Rhodothermales
- family: Salisaetaceae
- genus: Salisaeta
- species: Salisaeta longa
- full scientific name: Salisaeta longa Vaisman and Oren 2009
@ref: 15558
domain: Bacteria
phylum: Rhodothermaeota
class: Rhodothermia
order: Cytophagales
family: Salisaetaceae
genus: Salisaeta
species: Salisaeta longa
full scientific name: Salisaeta longa Vaisman and Oren 2009
strain designation: S4-4
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell shape | motility | confidence |
|---|---|---|---|---|---|
| 29324 | negative | 22.5 µm | rod-shaped | ||
| 69480 | no | 94.899 | |||
| 69480 | negative | 99.984 |
pigmentation
- @ref: 29324
- production: yes
Culture and growth conditions
culture medium
- @ref: 15558
- name: SALISAETA LONGA MEDIUM (DSMZ Medium 1221)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1221
- composition: Name: SALISAETA LONGA MEDIUM (DSMZ Medium 1221) Composition: NaCl 100.0 g/l MgCl2 x 6 H2O 50.0 g/l Agar 15.0 g/l K2SO4 5.0 g/l Starch 2.0 g/l Casamino acids 1.0 g/l Yeast extract 1.0 g/l CaCl2 x 2 H2O 0.1 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 15558 | positive | growth | 37 | mesophilic |
| 29324 | positive | growth | 25-50 | |
| 29324 | positive | optimum | 37-46 |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 29324 | positive | growth | 6.0-9.0 | alkaliphile |
| 29324 | positive | optimum | 6.5-8.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 29324
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.996 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 29324 | NaCl | positive | growth | 05-20 % |
| 29324 | NaCl | positive | optimum | 10 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29324 | 17234 | glucose | + | carbon source |
| 29324 | 17754 | glycerol | + | carbon source |
| 29324 | 17306 | maltose | + | carbon source |
| 29324 | 17992 | sucrose | + | carbon source |
metabolite production
- @ref: 29324
- Chebi-ID: 35581
- metabolite: indole
- production: yes
metabolite tests
- @ref: 29324
- Chebi-ID: 35581
- metabolite: indole
- indole test: +
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 29324 | catalase | + | 1.11.1.6 |
| 29324 | gelatinase | + |
Isolation, sampling and environmental information
isolation
- @ref: 15558
- sample type: experimental mesocosm filled with a mixture of water from the Dead Sea and the Red Sea
- geographic location: Dead Sea, Sodom
- country: Israel
- origin.country: ISR
- continent: Asia
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Marine
taxonmaps
- @ref: 69479
- File name: preview.99_5995.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_549;96_2859;97_3516;98_4448;99_5995&stattab=map
- Last taxonomy: Salisaeta longa subclade
- 16S sequence: EU426570
- Sequence Identity:
- Total samples: 165
- soil counts: 12
- aquatic counts: 140
- animal counts: 11
- plant counts: 2
Safety information
risk assessment
- @ref: 15558
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15558
- description: Salisaeta longa strain S4-4 16S ribosomal RNA gene, partial sequence
- accession: EU426570
- length: 1425
- database: ena
- NCBI tax ID: 503170
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Salisaeta longa DSM 21114 | GCA_000419585 | contig | ncbi | 1089550 |
| 66792 | Salisaeta longa DSM 21114 | 1089550.3 | wgs | patric | 1089550 |
| 66792 | Salisaeta longa DSM 21114 | 2517093009 | draft | img | 1089550 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 15558 | 62.9 | high performance liquid chromatography (HPLC) |
| 29324 | 62.9 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 95.966 | no |
| flagellated | no | 97.115 | no |
| gram-positive | no | 96.51 | yes |
| anaerobic | no | 98.026 | yes |
| aerobic | yes | 79.766 | yes |
| halophile | yes | 85.501 | yes |
| spore-forming | no | 92.779 | no |
| glucose-util | yes | 90.142 | no |
| thermophile | no | 71.078 | yes |
| glucose-ferment | no | 77.552 | no |
External links
@ref: 15558
culture collection no.: DSM 21114, CECT 7354
straininfo link
- @ref: 86789
- straininfo: 364184
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19622652 | Salisaeta longa gen. nov., sp. nov., a red, halophilic member of the Bacteroidetes. | Vaisman N, Oren A | Int J Syst Evol Microbiol | 10.1099/ijs.0.010892-0 | 2009 | Bacteroidetes/classification/genetics/*isolation & purification/*metabolism, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolism, *Water Microbiology | Metabolism |
| Phylogeny | 25888546 | Longimonas halophila gen. nov., sp. nov., isolated from a marine solar saltern. | Xia J, Zhou YX, Zhao LH, Chen GJ, Du ZJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.000247 | 2015 | Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, *Water Microbiology | Genetics |
| Phylogeny | 27226210 | Longibacter salinarum gen. nov., sp. nov., isolated from a marine solar saltern. | Xia J, Dunlap CA, Flor-Weiler L, Rooney AP, Chen GJ, Du ZJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001190 | 2016 | Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 15558 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21114) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21114 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 29324 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25736 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 86789 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID364184.1 | StrainInfo: A central database for resolving microbial strain identifiers |