Archive BacDiveID:10.13145/bacdive17783.20230509.8.1

Strain identifier

BacDive ID: 17783

Type strain:

Species: Cloacibacillus evryensis

Strain Designation: 158

Strain history: A. Sghir 158.

NCBI tax ID(s): 866499 (strain), 508460 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 8148

BacDive-ID: 17783

DSM-Number: 19522

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped

description: Cloacibacillus evryensis 158 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from sewage sludge.

NCBI tax id

NCBI tax id	Matching level
508460	species
866499	strain

strain history

@ref	history
8148	<- A. Sghir, Genoscope (CEA), Evry, France; 158 <- A. Ganesan {2006}
67770	A. Sghir 158.

doi: 10.13145/bacdive17783.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/synergistota
domain: Bacteria
phylum: Synergistota
class: Synergistia
order: Synergistales
family: Synergistaceae
genus: Cloacibacillus
species: Cloacibacillus evryensis
full scientific name: Cloacibacillus evryensis Ganesan et al. 2008

@ref: 8148

domain: Bacteria

phylum: Synergistetes

class: Synergistia

order: Synergistales

family: Synergistaceae

genus: Cloacibacillus

species: Cloacibacillus evryensis

full scientific name: Cloacibacillus evryensis Ganesan et al. 2008

strain designation: 158

type strain: yes

Morphology

cell morphology

@ref	gram stain	cell length	cell width	cell shape	motility	confidence
32534	negative	02-03 µm	0.8-1 µm	rod-shaped	no
69480	negative					99.806

pigmentation

@ref: 32534
production: yes

multimedia

@ref: 66793
multimedia content: EM_DSM_19522_1.jpg
caption: electron microscopic image
intellectual property rights: © HZI/Manfred Rohde

Culture and growth conditions

culture medium

@ref: 8148
name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)
growth: yes
link: https://mediadive.dsmz.de/medium/104c
composition: Name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Na2CO3 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
8148	positive	growth	37	mesophilic
32534	positive	growth	20-50
32534	positive	optimum	37	mesophilic
67770	positive	growth	37	mesophilic

culture pH

@ref	ability	type	pH	PH range
32534	positive	growth	6.5-10	alkaliphile
32534	positive	optimum	7

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance	confidence
8148	anaerobe
32534	anaerobe
69480	anaerobe	99.998

spore formation

@ref	spore formation	confidence
32534	no
69481	no	99
69480	no	99.992

halophily

@ref	salt	growth	tested relation	concentration
32534	NaCl	positive	growth	0-0.7 %
32534	NaCl	positive	optimum	0-0.7 %

observation

@ref: 32534
observation: aggregates in chains

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
32534	30089	acetate	+	carbon source
32534	17272	propionate	+	carbon source

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
8148	sewage sludge	wastewater treatment plant, Evry	France	FRA	Europe
67770	Anaerobic digester of a wastewater treatment plant in Evry		France	FRA	Europe

isolation source categories

Cat1: #Engineered
Cat2: #Waste
Cat3: #Sewage sludge

Safety information

risk assessment

@ref: 8148
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 8148
description: Cloacibacillus evryensis gen. nov. sp. nov. 16S ribosomal RNA gene partial sequence
accession: CU463952
length: 1334
database: ena
NCBI tax ID: 866499

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Cloacibacillus evryensis DSM 19522	866499.3	wgs	patric	866499
66792	Cloacibacillus evryensis 158, DSM 19522	2510065052	draft	img	866499
67770	Cloacibacillus evryensis DSM 19522	GCA_000585335	scaffold	ncbi	866499

GC content

@ref	GC-content	method
8148	55.8	high performance liquid chromatography (HPLC)
32534	55.8

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	no	99	no
motile	no	85.526	yes
flagellated	no	96.87	yes
gram-positive	no	97.68	no
anaerobic	yes	99.639	no
halophile	no	95.87	yes
spore-forming	no	90.012	yes
glucose-util	yes	81.378	no
aerobic	no	97.318	no
thermophile	no	80.367	no
glucose-ferment	no	63.668	no

External links

@ref: 8148

culture collection no.: DSM 19522, JCM 14828

straininfo link

@ref: 86774
straininfo: 405925

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	18768595	Cloacibacillus evryensis gen. nov., sp. nov., a novel asaccharolytic, mesophilic, amino-acid-degrading bacterium within the phylum 'Synergistetes', isolated from an anaerobic sludge digester.	Ganesan A, Chaussonnerie S, Tarrade A, Dauga C, Bouchez T, Pelletier E, Le Paslier D, Sghir A	Int J Syst Evol Microbiol	10.1099/ijs.0.65645-0	2008	Amino Acids/metabolism, Anaerobiosis, Bacteria/classification/genetics/isolation & purification/metabolism, Base Composition, Carbohydrate Metabolism, Culture Media/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Hydrogen-Ion Concentration, Locomotion, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sewage/*microbiology, Temperature	Metabolism
Metabolism	35887256	Identification of a Putative CodY Regulon in the Gram-Negative Phylum Synergistetes.	Geng J, Luo S, Shieh HR, Wang HY, Hu S, Chen YM	Int J Mol Sci	10.3390/ijms23147911	2022	Bacterial Proteins/metabolism, Gene Expression Regulation, Bacterial, Promoter Regions, Genetic, Regulon/genetics, Repressor Proteins/genetics

Reference

@id	authors	catalogue	doi/url	title	ID_cross_reference	pubmed	journal
8148	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19522)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-19522
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32534	Barberan A, Caceres Velazquez H, Jones S, Fierer N.		10.1128/mSphere.00237-17	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information	28752	28776041
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793	Mukherjee et al.		10.1038/nbt.3886	GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life		28604660	35: 676-683 2017
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
86774	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID405925.1	StrainInfo: A central database for resolving microbial strain identifiers