Strain identifier

BacDive ID: 17735

Type strain: Yes

Species: Cryptosporangium minutisporangium

Strain Designation: A-60

Strain history: ATCC 49415 <-- M. P. Lechevalier LL-A-60 <-- J. S. Ruan A-60.

NCBI tax ID(s): 113569 (species)

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General

@ref: 12033

BacDive-ID: 17735

DSM-Number: 44714

keywords: 16S sequence, Bacteria, mesophilic

description: Cryptosporangium minutisporangium A-60 is a mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 113569
  • Matching level: species

strain history

@refhistory
12033<- NBRC <- JCM <- ATCC <- M. P. Lechevalier, IMRU <- J.-S. Ruan; A-60
67770ATCC 49415 <-- M. P. Lechevalier LL-A-60 <-- J. S. Ruan A-60.

doi: 10.13145/bacdive17735.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Cryptosporangiales
  • family: Cryptosporangiaceae
  • genus: Cryptosporangium
  • species: Cryptosporangium minutisporangium
  • full scientific name: Cryptosporangium minutisporangium (Ruan et al. 1986) Tamura and Hatano 2001

  • synonyms

    • @ref: 20215
    • synonym: Actinoplanes minutisporangius

@ref: 12033

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cryptosporangiaceae

genus: Cryptosporangium

species: Cryptosporangium minutisporangium

full scientific name: Cryptosporangium minutisporangium (Ruan et al. 1986) Tamura and Hatano 2001

strain designation: A-60

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
20104Sepia brown (8014)10-14 daysISP 2
20104Bottle green (6007)10-14 daysISP 3
20104Sepia brown (8014)10-14 daysISP 4
20104Sepia brown (8014)10-14 daysISP 5
20104Pastel orange (2003)/Sepia brown (8014)10-14 daysISP 6
20104Sepia brown (8014)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
20104noISP 2
20104noISP 3
20104noISP 4
20104noISP 5
20104noISP 6
20104noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12033GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20104ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20104ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20104ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20104ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20104ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20104ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
12033ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
12033positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2), MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activity
2010417234glucose+
2010422599arabinose+
2010417992sucrose+
2010418222xylose+
2010417268myo-inositol+
2010429864mannitol+
2010428757fructose+
2010426546rhamnose+
2010416634raffinose+
2010462968cellulose+

enzymes

@refvalueactivityec
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20104+++-+--+-+++++++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12033soilBeijingChinaCHNAsia
67770SoilChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_1579.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_94;96_898;97_1047;98_1246;99_1579&stattab=map
  • Last taxonomy: Cryptosporangium
  • 16S sequence: AB048220
  • Sequence Identity:
  • Total samples: 2846
  • soil counts: 2031
  • aquatic counts: 220
  • animal counts: 351
  • plant counts: 244

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
120331Risk group (German classification)
201041German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinoplanes minutisporangius gene for 16S rRNA, partial sequenceAB0370071470ena113569
20218Actinoplanes minutisporangius gene for 16S ribosomal RNA, partial sequenceAB0482201438ena113569

GC content

  • @ref: 67770
  • GC-content: 75.8
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 12033

culture collection no.: DSM 44714, ATCC 49415, IFO 15962, IMRU LL-A-60, JCM 9458, KCTC 9642, NBRC 15962, CGMCC 4.1185, IFO 16112, NBRC 16112

straininfo link

  • @ref: 86725
  • straininfo: 42458

literature

  • topic: Phylogeny
  • Pubmed-ID: 11760955
  • title: Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and 'Actinoplanes aurantiacus' to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov.
  • authors: Tamura T, Hatano K
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-6-2119
  • year: 2001
  • mesh: Actinomycetales/chemistry/*classification/genetics, DNA, Ribosomal/genetics, Micromonosporaceae/chemistry/*classification/*genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12033Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44714)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44714
20104Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44714.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86725Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42458.1StrainInfo: A central database for resolving microbial strain identifiers