Strain identifier
BacDive ID: 17729
Type strain:
Species: Actinopolyspora alba
Strain history: <- S.-K. Tang; YIM 90480
NCBI tax ID(s): 673379 (species)
General
@ref: 12236
BacDive-ID: 17729
DSM-Number: 45004
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic
description: Actinopolyspora alba DSM 45004 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil from a salt field.
NCBI tax id
- NCBI tax id: 673379
- Matching level: species
strain history
- @ref: 12236
- history: <- S.-K. Tang; YIM 90480
doi: 10.13145/bacdive17729.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Actinopolyspora
- species: Actinopolyspora alba
- full scientific name: Actinopolyspora alba Tang et al. 2011
@ref: 12236
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Actinopolysporaceae
genus: Actinopolyspora
species: Actinopolyspora alba
full scientific name: Actinopolyspora alba Tang et al. 2011
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 94.217 | |
69480 | 100 | positive |
colony morphology
@ref | colony color | medium used |
---|---|---|
69370 | Ochre yellow (1024) | ISP 2 |
69370 | Ochre yellow (1024) | ISP 4 |
69370 | Ochre yellow (1024) | ISP 6 |
69370 | Oyster white (1013) | ISP 5 |
69370 | Oyster white (1013) | ISP 7 |
69370 | Oyster white (1013) | suter with tyrosine |
69370 | Oyster white (1013) | suter without tyrosine |
multicellular morphology
@ref | forms multicellular complex | complex name | complex color | medium name |
---|---|---|---|---|
69370 | yes | Aerial mycelium | Traffic white (9016) | ISP 2 |
69370 | yes | Aerial mycelium | Traffic white (9016) | ISP 4 |
69370 | no | Aerial mycelium | ISP 5 | |
69370 | yes | Aerial mycelium | Traffic white (9016) | ISP 6 |
69370 | no | Aerial mycelium | ISP 7 | |
69370 | no | Aerial mycelium | suter with tyrosine | |
69370 | no | Aerial mycelium | suter without tyrosine |
pigmentation
@ref | production | name |
---|---|---|
69370 | no | Melanin |
69370 | no | soluble pigment |
multimedia
@ref | multimedia content | caption | intellectual property rights |
---|---|---|---|
12236 | https://www.dsmz.de/microorganisms/photos/DSM_45004.jpg | Medium 1241 37°C | © Leibniz-Institut DSMZ |
69370 | DSM_45004_image3.jpeg | Plates (1241, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
69370 | DSM_45004_image4.jpeg | Plates (1241, ISP2, ISP3, ISP4, ISP5, ISP7) | Helmholtz-Zentrum für Infektionsforschung GmbH |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
12236 | STARCH-MINERAL SALT-AGAR (STMS) + 15% NACL (DSMZ Medium 1241) | yes | https://mediadive.dsmz.de/medium/1241 | Name: STARCH - MINERAL SALT - AGAR (STMS) + 15% NaCl (DSMZ Medium 1241) Composition: NaCl 150.0 g/l Agar 15.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water |
12236 | STARCH-MINERAL SALT-AGAR (STMS) + 10%NACL (DSMZ Medium 1240) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1240.pdf |
culture temp
- @ref: 12236
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 96 |
69480 | yes | 100 |
halophily
- @ref: 69370
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 5-10 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
69370 | 22599 | arabinose | - | growth |
69370 | 62968 | cellulose | - | growth |
69370 | 28757 | fructose | + | growth |
69370 | 17234 | glucose | + | growth |
69370 | 17268 | inositol | - | growth |
69370 | 37684 | mannose | + | growth |
69370 | 16634 | raffinose | - | growth |
69370 | 26546 | rhamnose | - | growth |
69370 | 17992 | sucrose | - | growth |
69370 | 18222 | xylose | + | growth |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | - | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68379 | gelatinase | + | |
68379 | urease | - | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69370 | - | - | - | + | - | - | + | + | - | - | + | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
69370 | + | + | + | +/- | + | +/- | - | - | - | - | - | - | - | - | +/- | - | + | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 12236
- sample type: soil from a salt field
- geographic location: Xinjiang Province, Baicheng salt field
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Agriculture | #Field |
#Environmental | #Terrestrial | #Soil |
#Condition | #Saline |
Safety information
risk assessment
- @ref: 12236
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 12236
- description: Actinopolyspora alba strain YIM 90480 16S ribosomal RNA gene, partial sequence
- accession: GQ480940
- length: 1543
- database: ena
- NCBI tax ID: 673379
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Actinopolyspora alba DSM 45004 | GCA_900112765 | scaffold | ncbi | 673379 |
66792 | Actinopolyspora alba strain DSM 45004 | 673379.3 | wgs | patric | 673379 |
66792 | Actinopolyspora alba DSM 45004 | 2619619028 | draft | img | 673379 |
GC content
- @ref: 12236
- GC-content: 68.3
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 96 | no |
motile | no | 91.707 | no |
flagellated | no | 97.075 | no |
gram-positive | yes | 90.217 | no |
anaerobic | no | 98.771 | no |
aerobic | yes | 88.969 | no |
halophile | yes | 68.681 | no |
spore-forming | yes | 80.568 | no |
glucose-ferment | no | 88.96 | yes |
thermophile | no | 92.616 | no |
glucose-util | yes | 88.982 | no |
External links
@ref: 12236
culture collection no.: DSM 45004, KCTC 19119, YIM 90480
straininfo link
- @ref: 86719
- straininfo: 297481
literature
- topic: Phylogeny
- Pubmed-ID: 20729305
- title: Actinopolyspora alba sp. nov. and Actinopolyspora erythraea sp. nov., isolated from a salt field, and reclassification of Actinopolyspora iraqiensis Ruan et al. 1994 as a heterotypic synonym of Saccharomonospora halophila.
- authors: Tang SK, Wang Y, Klenk HP, Shi R, Lou K, Zhang YJ, Chen C, Ruan JS, Li WJ
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.022319-0
- year: 2010
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salts/chemistry, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistry
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
12236 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45004) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45004 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
68379 | Automatically annotated from API Coryne | |||
68382 | Automatically annotated from API zym | |||
69370 | Wink, J. | https://cdn.dsmz.de/wink/DSM%2045004.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
86719 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID297481.1 | StrainInfo: A central database for resolving microbial strain identifiers |