Strain identifier

BacDive ID: 17721

Type strain: Yes

Species: Actinopolyspora halophila

Strain history: KCC A-0278 <-- ATCC 27976 <-- T. J. Novitsky <-- M. Gochnauer.

NCBI tax ID(s): 1089546 (strain), 1850 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 11318

BacDive-ID: 17721

DSM-Number: 43834

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Actinopolyspora halophila DSM 43834 is a spore-forming, mesophilic bacterium that was isolated from dairy salt.

NCBI tax id

NCBI tax idMatching level
1089546strain
1850species

strain history

@refhistory
11318<- KCC <- ATCC <- T.J. Novitsky <- M. Gochnauer
67770KCC A-0278 <-- ATCC 27976 <-- T. J. Novitsky <-- M. Gochnauer.

doi: 10.13145/bacdive17721.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Actinopolyspora
  • species: Actinopolyspora halophila
  • full scientific name: Actinopolyspora halophila Gochnauer et al. 1975 (Approved Lists 1980)

@ref: 11318

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinopolysporaceae

genus: Actinopolyspora

species: Actinopolyspora halophila

full scientific name: Actinopolyspora halophila Gochnauer et al. 1975 emend. Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no95.024
69480100positive

multicellular morphology

@refforms multicellular complexmedium name
19602noISP 2
19602noISP 3
19602noISP 4
19602noISP 5
19602noISP 6
19602noISP 7

multimedia

@refmultimedia contentintellectual property rightscaption
11318https://www.dsmz.de/microorganisms/photos/DSM_43834-1.jpg© Leibniz-Institut DSMZ
11318https://www.dsmz.de/microorganisms/photos/DSM_43834.jpg© Leibniz-Institut DSMZMedium 549 37°C
66793EM_DSM_43834_1.jpg© HZI/Manfred Rohdeelectron microscopic image
66793EM_DSM_43834_2.jpg© HZI/Manfred Rohdeelectron microscopic image
66793EM_DSM_43834_3.jpg© HZI/Manfred Rohdeelectron microscopic image
66793EM_DSM_43834_4.jpg© HZI/Manfred Rohdeelectron microscopic image
66793EM_DSM_43834_5.jpg© HZI/Manfred Rohdeelectron microscopic image

Culture and growth conditions

culture medium

  • @ref: 11318
  • name: CM + YE MEDIUM (DSMZ Medium 549)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/549
  • composition: Name: CM + YE MEDIUM (DSMZ Medium 549) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Casamino acids 7.5 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l FeSO4 x 7 H2O 0.01 g/l HCl 3.646e-05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
11318positivegrowth37mesophilic
19602positiveoptimum37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

spore formation

@refspore descriptiontype of sporespore formationconfidence
19602Formation of spores, spore surface smoothsporeyes
69481yes100
69480yes99.655

compound production

  • @ref: 20216
  • compound: ß-lactamase

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-9(H4), MK-10(H4)

enzymes

  • @ref: 67770
  • value: beta-lactamase
  • ec: 3.5.2.6

Isolation, sampling and environmental information

isolation

@refsample typehost species
11318dairy salt
67770Contaminant of complex medium for HalobacteriumHalobacterium

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_4945.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_495;96_2401;97_2923;98_3701;99_4945&stattab=map
  • Last taxonomy: Actinopolyspora halophila
  • 16S sequence: X54287
  • Sequence Identity:
  • Total samples: 37
  • soil counts: 13
  • aquatic counts: 4
  • animal counts: 20

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
113181Risk group (German classification)
196021Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218A.halophila partial 16S rRNAX542871476ena1850
20218A.halophila (JCM3278T) DNA for 16S-23S rRNA intergenic spacerZ46603271ena1850

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinopolyspora halophila DSM 438341089546.3wgspatric1089546
66792Actinopolyspora halophila DSM 438342517287026draftimg1089546
67770Actinopolyspora halophila DSM 43834GCA_000371785contigncbi1089546

GC content

@refGC-contentmethod
6777068genome sequence analysis
6777068high performance liquid chromatography (HPLC)
6777064.2fluorimetric

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno90.123no
flagellatedno97.422no
gram-positiveyes92.538no
anaerobicno98.879no
aerobicyes90.361no
halophileyes76.393no
spore-formingyes76.635no
glucose-utilyes89.168no
thermophileno92.858no
glucose-fermentno89.15no

External links

@ref: 11318

culture collection no.: DSM 43834, ATCC 27976, IFO 14106, JCM 3278, KCC A-0278, NBRC 14106, NCIB 11472, BCRC 13403, CGMCC 4.1219, IMSNU 20045, IMSNU 21360, KCTC 9127, MTCC 263, NCIMB 11472, VKM Ac-871

straininfo link

  • @ref: 86712
  • straininfo: 39285

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23196893Actinopolyspora saharensis sp. nov., a novel halophilic actinomycete isolated from a Saharan soil of Algeria.Meklat A, Bouras N, Zitouni A, Mathieu F, Lebrihi A, Schumann P, Sproer C, Klenk HP, Sabaou NAntonie Van Leeuwenhoek10.1007/s10482-012-9859-z2012Actinomycetales/*classification/growth & development/*isolation & purification, Algeria, Bacterial Typing Techniques, Carbohydrates/analysis, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Temperature, Vitamin K 2/analysisGenetics
Phylogeny25429761Actinopolyspora biskrensis sp. nov., a novel halophilic actinomycete isolated from Northern Sahara.Saker R, Bouras N, Meklat A, Zitouni A, Schumann P, Sproer C, Klenk HP, Sabaou NCurr Microbiol10.1007/s00284-014-0740-32014Actinomycetaceae/*classification/genetics/isolation & purification/metabolism, Africa, Northern, Metabolomics, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAPhenotype
28626687Investigations on ideal mode of cell disruption in extremely halophilic Actinopolyspora halophila (MTCC 263) for efficient release of glycine betaine and trehalose.Kar JR, Singhal RSBiotechnol Rep (Amst)10.1016/j.btre.2014.12.0052014

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11318Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43834)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43834
19602Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43834.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86712Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39285.1StrainInfo: A central database for resolving microbial strain identifiers