Strain identifier

BacDive ID: 17714

Type strain: Yes

Species: Aquiflexum balticum

Strain Designation: BA160

Strain history: CIP <- 2004, I. Brettar, GBF, Braunschweig, Germany: strain BA160

NCBI tax ID(s): 758820 (strain), 280473 (species)

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General

@ref: 6475

BacDive-ID: 17714

DSM-Number: 16537

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Aquiflexum balticum BA160 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from water sample .

NCBI tax id

NCBI tax idMatching level
280473species
758820strain

strain history

@refhistory
6475<- I. Brettar; BA160
332432004, I. Brettar, GBF, Braunschweig, Germany: strain BA160
116724CIP <- 2004, I. Brettar, GBF, Braunschweig, Germany: strain BA160

doi: 10.13145/bacdive17714.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Cytophagia
  • order: Cytophagales
  • family: Cyclobacteriaceae
  • genus: Aquiflexum
  • species: Aquiflexum balticum
  • full scientific name: Aquiflexum balticum Brettar et al. 2004

@ref: 6475

domain: Bacteria

phylum: Bacteroidetes

class: Cytophagia

order: Cytophagales

family: Cyclobacteriaceae

genus: Aquiflexum

species: Aquiflexum balticum

full scientific name: Aquiflexum balticum Brettar et al. 2004 emend. Bhumika et al. 2013

strain designation: BA160

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31302negative3 µm0.45 µmrod-shaped
69480no92.135
69480negative99.977
116724negativerod-shapedno

multimedia

  • @ref: 66793
  • multimedia content: EM_DSM_16537_1.jpg
  • caption: electron microscopic image
  • intellectual property rights: © HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6475HALF STRENGTH BACTO MARINE BROTH (DSMZ Medium 514a)yeshttps://mediadive.dsmz.de/medium/514aName: HALF STRENGTH BACTO MARINE BROTH (DSMZ Medium 514a) Composition: Agar 15.0 g/l NaCl 9.725 g/l MgCl2 2.95 g/l Bacto peptone 2.5 g/l Na2SO4 1.62 g/l CaCl2 0.9 g/l Yeast extract 0.5 g/l KCl 0.275 g/l NaHCO3 0.08 g/l Fe(III) citrate 0.05 g/l KBr 0.04 g/l SrCl2 0.017 g/l H3BO3 0.011 g/l Na2HPO4 0.004 g/l Na-silicate 0.002 g/l NaF 0.0012 g/l (NH4)NO3 0.0008 g/l Distilled water
33243Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
116724CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
6475positivegrowth30mesophilic
31302positivegrowth04-40
31302positiveoptimum30mesophilic
33243positivegrowth30mesophilic
116724positivegrowth10-30
116724nogrowth37mesophilic
116724nogrowth41thermophilic

culture pH

@refabilitytypepHPH range
31302positivegrowth07-09alkaliphile
31302positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 31302
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.895

halophily

@refsaltgrowthtested relationconcentration
31302NaClpositivegrowth0-6 %
31302NaClpositiveoptimum1.5 %
116724NaClpositivegrowth0-6 %
116724NaClnogrowth8 %
116724NaClnogrowth10 %

observation

  • @ref: 31302
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3130230089acetate+carbon source
3130222599arabinose+carbon source
3130217057cellobiose+carbon source
3130228757fructose+carbon source
3130233984fucose+carbon source
3130228260galactose+carbon source
3130224265gluconate+carbon source
3130217234glucose+carbon source
3130224996lactate+carbon source
3130217716lactose+carbon source
3130225115malate+carbon source
3130217306maltose+carbon source
3130237684mannose+carbon source
31302506227N-acetylglucosamine+carbon source
3130230911sorbitol+carbon source
3130217992sucrose+carbon source
3130227082trehalose+carbon source
3130217632nitrate+reduction
6836817632nitrate-reduction
6836817634D-glucose-fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
11672417108D-arabinose-degradation
11672415824D-fructose-degradation
11672417634D-glucose-degradation
11672416024D-mannose-degradation
11672465327D-xylose-degradation
11672417057cellobiose-degradation
11672417716lactose-degradation
11672417306maltose-degradation
11672417814salicin-degradation
11672417992sucrose-degradation
11672416947citrate-carbon source
1167244853esculin+hydrolysis
11672417632nitrate+reduction
11672416301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836817997dinitrogenno
6836816301nitriteno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11672435581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
31302catalase+1.11.1.6
31302gelatinase+
31302cytochrome oxidase+1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
116724oxidase+
116724beta-galactosidase-3.2.1.23
116724alcohol dehydrogenase-1.1.1.1
116724gelatinase+/-
116724amylase+
116724caseinase-3.4.21.50
116724catalase+1.11.1.6
116724tween esterase+
116724lecithinase-
116724lipase-
116724lysine decarboxylase-4.1.1.18
116724ornithine decarboxylase-4.1.1.17
116724tryptophan deaminase-
116724urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116724-+++-+++++++---+-+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUNO2N2
116724--------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116724-------------+----+--------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryisolation date
6475water sample (5 m depth)central Baltic Sea (international area)
116724Environment, Baltic sea watercentralBaltic Sea1998

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_4498.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15774;96_2250;97_2724;98_3382;99_4498&stattab=map
  • Last taxonomy: Aquiflexum balticum subclade
  • 16S sequence: AJ744861
  • Sequence Identity:
  • Total samples: 269
  • soil counts: 31
  • aquatic counts: 144
  • animal counts: 90
  • plant counts: 4

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
64751Risk group (German classification)
1167241Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6475
  • description: Aquiflexum balticum 16S rRNA gene, type strain BA160
  • accession: AJ744861
  • length: 1459
  • database: ena
  • NCBI tax ID: 758820

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aquiflexum balticum DSM 16537GCA_900176595chromosomencbi758820
66792Aquiflexum balticum DSM 16537 strain DSM 16537758820.4completepatric758820
66792Aquiflexum balticum BA160, DSM 165372506520046completeimg758820

GC content

  • @ref: 31302
  • GC-content: 38.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno91.079no
flagellatedno96.938no
gram-positiveno97.039yes
anaerobicno98.275yes
aerobicyes86.875yes
halophileno88.502yes
spore-formingno95.152no
glucose-utilyes87.252yes
glucose-fermentno89.582no
thermophileno98.678yes

External links

@ref: 6475

culture collection no.: DSM 16537, CIP 108445, LMG 22565

straininfo link

  • @ref: 86705
  • straininfo: 132334

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15545480Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea.Brettar I, Christen R, Hofle MGInt J Syst Evol Microbiol10.1099/ijs.0.63255-02004Bacterial Typing Techniques, Base Composition, Carotenoids/isolation & purification, Catalase/analysis, Cytophagaceae/*classification/cytology/*isolation & purification/physiology, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis/isolation & purification, Genes, rRNA, Gentian Violet, Molecular Sequence Data, Oxidoreductases/analysis, Phenazines, Phylogeny, Pigments, Biological, Plankton/microbiology, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Saline Solution, Hypertonic, Seawater/*microbiology, Sequence Analysis, DNA, Temperature, Water MicrobiologyEnzymology
Phylogeny23064355Mariniradius saccharolyticus gen. nov., sp. nov., a member of the family Cyclobacteriaceae isolated from marine aquaculture pond water, and emended descriptions of the genus Aquiflexum and Aquiflexum balticum.Bhumika V, Srinivas TNR, Ravinder K, Anil Kumar PInt J Syst Evol Microbiol10.1099/ijs.0.043919-02012*Aquaculture, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Gram-Negative Aerobic Rods and Cocci/*classification/genetics/isolation & purification, India, Molecular Sequence Data, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysis, Water MicrobiologyGenetics
Phylogeny33763765Aquiflexum lacus sp. nov., isolated from a lake sediment sample.Huang J, Han M, Fang B, Yang J, Xiao H, Zhang X, Han J, Yu D, Jiang H, Li WArch Microbiol10.1007/s00203-021-02280-z2021Bacteroidetes/classification/*isolation & purification, Geologic Sediments/*microbiology, Lakes/*microbiology, PhylogenyEnzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
6475Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16537)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16537
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31302Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2762028776041
33243Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6068
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life28604660 35: 676-683 2017
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86705Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID132334.1StrainInfo: A central database for resolving microbial strain identifiers
116724Curators of the CIPCollection of Institut Pasteur (CIP 108445)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108445