Strain identifier

BacDive ID: 17679

Type strain: Yes

Species: Anaerotruncus colihominis

Strain Designation: 277

Strain history: CIP <- 2002, CCUG <- H. Sarah, London, UK <- D. Molitoris, Sydney Finegold, California, USA: strain 277 <- Finegold: strain WAL 14565

NCBI tax ID(s): 445972 (strain), 169435 (species)

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General

@ref: 6848

BacDive-ID: 17679

DSM-Number: 17241

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Anaerotruncus colihominis 277 is an anaerobe, mesophilic, Gram-positive bacterium that forms irregular colonies and was isolated from human feces, autistic child.

NCBI tax id

NCBI tax idMatching level
445972strain
169435species

strain history

@refhistory
6848<- CCUG <-P.A. Lawson, UK <-D.Molitoris, S.Finegold, USA; WAL 14565 <- Rush Childrens Hospital
67770CCUG 45055 <-- P. A. Lawson <-- H. N. Shah <-- D. R. Molitoris <-- S. M. Finegold WAL 14565.
118701CIP <- 2002, CCUG <- H. Sarah, London, UK <- D. Molitoris, Sydney Finegold, California, USA: strain 277 <- Finegold: strain WAL 14565

doi: 10.13145/bacdive17679.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Oscillospiraceae
  • genus: Anaerotruncus
  • species: Anaerotruncus colihominis
  • full scientific name: Anaerotruncus colihominis Lawson et al. 2004

@ref: 6848

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Oscillospiraceae

genus: Anaerotruncus

species: Anaerotruncus colihominis

full scientific name: Anaerotruncus colihominis Lawson et al. 2004

strain designation: 277

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
29914positive3.5 µm0.5 µmrod-shapedno
26285positive2-5 µm0.5 µmrod-shapedno
69480positive99.999
118701positiverod-shapedyes

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium used
262852-3 mmgreyirregular2 daysBrucella blood agar (Anaerobe Systems)
118701

pigmentation

  • @ref: 29914
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6848BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)yeshttps://mediadive.dsmz.de/medium/58Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water
6848COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
33163MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
26285Brucella blood agar (Anaerobe Systems)yes
118701CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
6848positivegrowth37mesophilic
29914positivegrowth36-40
29914positiveoptimum37mesophilic
33163positivegrowth37mesophilic
56619positivegrowth37mesophilic
67770positivegrowth37mesophilic
118701positivegrowth37mesophilic

culture pH

  • @ref: 29914
  • ability: positive
  • type: growth
  • pH: 5.5-11
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
6848anaerobe
29914anaerobe
56619anaerobe
69480anaerobe99.999
118701anaerobe

spore formation

@refspore formationconfidence
29914no
26285no
69481no96
69480no97.025
118701yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2991417057cellobiose+carbon source
2991428757fructose+carbon source
2991428260galactose+carbon source
299145291gelatin+carbon source
2991417234glucose+carbon source
2991417306maltose+carbon source
2991437684mannose+carbon source
2991427082trehalose+carbon source
2628515589L-malate+assimilation
26285172562-deoxyadenosine+assimilation
26285167632-oxobutanoate+assimilation
26285286442-oxopentanoate+assimilation
26285739183-O-methyl-D-glucose+assimilation
2628517925alpha-D-glucose+assimilation
2628522599arabinose-growth
2628518305arbutin+growth
2628562968cellulose-hydrolysis
2628562968cellulose+growth
2628517057cellobiose+assimilation
2628515824D-fructose+assimilation
2628512936D-galactose+assimilation
2628518024D-galacturonic acid+assimilation
2628516024D-mannose+assimilation
2628516551D-trehalose+assimilation
2628523652dextrin+assimilation
262854853esculin-hydrolysis
2628528757fructose+growth
262855291gelatin-hydrolysis
2628517234glucose+growth
2628517596inosine+assimilation
2628517268myo-inositol-growth
2628516414L-valine+assimilation
2628517716lactose-growth
2628517306maltose-growth
2628517306maltose+assimilation
2628561993maltotriose+assimilation
2628529864mannitol-growth
2628537684mannose+growth
262856731melezitose-growth
2628528053melibiose-growth
2628517540methyl beta-D-galactoside+assimilation
26285320055methyl beta-D-glucopyranoside+assimilation
2628551850methyl pyruvate+assimilation
2628563153N-acetyl-D-mannosamine+assimilation
26285506227N-acetylglucosamine+assimilation
2628517632nitrate-reduction
2628518394palatinose+assimilation
26285peptone-growth
2628515361pyruvate+assimilation
2628516634raffinose-growth
2628526546rhamnose-growth
2628533942ribose-growth
2628517814salicin-growth
2628517822serine+assimilation
2628530911sorbitol-growth
2628528017starch-growth
2628517748thymidine+assimilation
2628532528turanose+assimilation
2628516199urea-hydrolysis
2628516704uridine+assimilation
2628518222xylose-growth
11870117108D-arabinose-degradation
11870115824D-fructose+degradation
11870117634D-glucose+degradation
11870165327D-xylose-degradation
11870117057cellobiose-degradation
11870117716lactose-degradation
11870117306maltose-degradation
11870117814salicin-degradation
11870117992sucrose+degradation
1187014853esculin-hydrolysis
11870117632nitrate-reduction
11870116301nitrite-reduction
11870117632nitrate+respiration
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
262856104kanamycinyesyes1000 µg (disc)
2628528001vancomycinyesyes5 µg (disc)
2628537943colistinyesyes10 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
2991435581indoleyes
2628530089acetateyes
2628517968butyrateyes
2628535581indoleyes
11870135581indoleyes

metabolite tests

  • @ref: 29914
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
26285catalase-1.11.1.6
26285acid phosphatase+3.1.3.2
26285N-acetyl-beta-glucosaminidase-3.2.1.52
26285alkaline phosphatase-3.1.3.1
26285arginine arylamidase-
26285arginine dihydrolase-3.5.3.6
26285alpha-arabinosidase-3.2.1.55
26285esterase Lipase (C 8)-
26285alpha-glucosidase-3.2.1.20
26285beta-glucosidase-3.2.1.21
26285beta-glucuronidase-3.2.1.31
26285glutamyl-glutamate arylamidase-
26285alpha-mannosidase-3.2.1.24
26285alpha-fucosidase-3.2.1.51
26285chymotrypsin-3.4.4.5
26285leucyl glycin arylamidase-3.4.11.1
26285phosphoamidase-3.9.1.1
26285trypsin-3.4.21.4
26285cystine arylamidase-3.4.11.3
26285esterase (C 4)-
262856-phospho-beta-galactosidase-3.2.1.85
26285glutamate decarboxylase-4.1.1.15
26285glycin arylamidase-
26285histidine arylamidase-
26285lipase (C 14)-
26285leucine arylamidase-3.4.11.1
26285phenylalanine arylamidase-
26285proline-arylamidase-3.4.11.5
26285pyroglutamic acid arylamidase-
26285serine arylamidase-
26285tyrosine arylamidase-
26285valine arylamidase-
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
118701oxidase-
118701beta-galactosidase-3.2.1.23
118701gelatinase-
118701catalase-1.11.1.6
118701gamma-glutamyltransferase-2.3.2.2
118701urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118701--++------++----+---

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
6848---------------+-------------
6848------+---------+------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6848human feces, autistic childChicagoUSAUSANorth America
26285faecal material of a 71-month old autistic child from the Rushs Childrens HospitalChicagoUSAUSANorth America
56619Human feces from autistic childCaliforniaUSAUSANorth America
67770Human feces
118701Human, FecesUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Human#Child
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_5477.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_154;96_2651;97_3251;98_4090;99_5477&stattab=map
  • Last taxonomy: Anaerotruncus colihominis subclade
  • 16S sequence: AJ315980
  • Sequence Identity:
  • Total samples: 53235
  • soil counts: 286
  • aquatic counts: 361
  • animal counts: 52440
  • plant counts: 148

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
68481Risk group (German classification)
1187011Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6848
  • description: Ruminococcus sp. 14565 partial 16S rRNA gene, strain 14565
  • accession: AJ315980
  • length: 1393
  • database: ena
  • NCBI tax ID: 445972

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerotruncus colihominis DSM 17241445972.6wgspatric445972
66792Anaerotruncus colihominis DSM 17241641736271draftimg445972
67770Anaerotruncus colihominis DSM 17241 DSM 17241GCA_000154565scaffoldncbi445972
66792Anaerotruncus colihominis DSM 17241GCA_025146135completencbi169435
66792Anaerotruncus colihominis strain DSM 17241169435.95completepatric169435

GC content

@refGC-contentmethod
684854
2628554high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno96no
motileyes60.268yes
flagellatedno87.78yes
gram-positiveyes87.569yes
anaerobicyes99.526no
aerobicno97.298yes
halophileno91.391no
spore-formingno76.051yes
glucose-fermentyes63.438no
thermophileno92.541yes
glucose-utilyes85.718yes

External links

@ref: 6848

culture collection no.: DSM 17241, CCUG 45055, CIP 107754, WAL 14565, JCM 15631, VTT E-062942

straininfo link

  • @ref: 86670
  • straininfo: 131413

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15023953Anaerotruncus colihominis gen. nov., sp. nov., from human faeces.Lawson PA, Song Y, Liu C, Molitoris DR, Vaisanen ML, Collins MD, Finegold SMInt J Syst Evol Microbiol10.1099/ijs.0.02653-02004Base Composition, Clostridium/*classification/genetics/isolation & purification/ultrastructure, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Feces/*microbiology, Humans, Microscopy, Electron, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/geneticsPathogenicity
Phylogeny27432404Harryflintia acetispora gen. nov., sp. nov., isolated from chicken caecum.Petzoldt D, Breves G, Rautenschlein S, Taras DInt J Syst Evol Microbiol10.1099/ijsem.0.0013172016Animals, Bacterial Typing Techniques, Base Composition, Cecum/*microbiology, Chickens/*microbiology, Clostridiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Phylogeny28901896Paludicola psychrotolerans gen. nov., sp. nov., a novel psychrotolerant chitinolytic anaerobe of the family Ruminococcaceae.Li Y, Zhang LL, Liu L, Tian YQ, Liu XF, Li WJ, Dai YMInt J Syst Evol Microbiol10.1099/ijsem.0.0022602017Bacterial Typing Techniques, Base Composition, China, Chitin/metabolism, Clostridiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *WetlandsTranscriptome
Phylogeny30891246Anaerotruncus massiliensis sp. nov., a succinate-producing bacterium isolated from human stool from an obese patient after bariatric surgery.Togo AH, Diop A, Dubourg G, Khelaifia S, Richez M, Armstrong N, Maraninchi M, Fournier PE, Raoult D, Million MNew Microbes New Infect10.1016/j.nmni.2019.01.0042019
34497657Cornuside Alleviates Diabetes Mellitus-Induced Testicular Damage by Modulating the Gut Microbiota.Liu L, Shu A, Zhu Y, Chen YEvid Based Complement Alternat Med10.1155/2021/53019422021
Pathogenicity36045662Sodium Butyrate Attenuated Diabetes-Induced Intestinal Inflammation by Modulating Gut Microbiota.Liu L, Chen Y, Wu Q, Shu A, Sun JEvid Based Complement Alternat Med10.1155/2022/46462452022
Phylogeny36149539Draft genomes and descriptions of Urmitella timonensis gen. nov., sp. nov. and Marasmitruncus massiliensis gen. nov., sp. nov., isolated from severely malnourished African children using culturomics.Bellali S, Haddad G, Pham TP, Iwaza R, Ibrahim A, Armstrong N, Fadlane A, Couderc C, Diallo A, Sokhna C, Million M, Raoult D, Tidjani Alou MAntonie Van Leeuwenhoek10.1007/s10482-022-01777-x2022Humans, Child, Female, *RNA, Ribosomal, 16S/genetics, DNA, Bacterial/genetics, PhylogenyPathogenicity

Reference

@idauthorscataloguedoi/urltitlepubmedID_cross_referencejournal
6848Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17241)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17241
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
2628510.1099/ijs.0.02653-015023953
29914Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126285
33163Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5224
56619Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45055)https://www.ccug.se/strain?id=45055
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86670Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID131413.1StrainInfo: A central database for resolving microbial strain identifiers
118701Curators of the CIPCollection of Institut Pasteur (CIP 107754)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107754